EM122 Water Column Sonar Data Collected During AT26-10
AT26-10 Chemosynthetic Microbial Communities at Deep-Sea Vents (EM122). The cruise will include 16 dives with DSV Alvin or ROV Jason, to allow time for deployment and collection of experiments and for extensive sampling of discrete vents along the axis of the 9°46’N to 9°53’N segment of the East Pacific Rise (EPR). We propose to deploy experiments/sample a low temperature diffuse flow vent (e.g., Crab Spa; T: 10-30°C), and a higher temperature vent (e.g., L-vent; T: 30-60°C). Furthermore, we will carry out additional opportunistic sampling for a limited set of parameters (e.g., chemistry, 454- pyrotags, FISH, in situ carbon fixation) of vents that fall into these two categories, or that might have developed since the last visit to the site (May 2012). During the course of the cruise we will collect biomass from fluids and biofilms (deployment/recover experimental microbial colonizers) by utilizing a Large Volume Pump (LVP) (McLane WTS 6-1-142LV). We have successfully used this set up in previous cruises and were able to collect up to ~2,000 liters of hydrothermal fluids resulting in enough biomass (cell density is 2-5 x 105 cells per ml) for subsequent ‘omic’ analyses. During large volume filtration, the volume filtered is recorded by the LVP onboard computer and the vent fluid sampled is monitored with a time series temperature probe, H2S, and pH sensors. Fluid samples for chemical analyses will be collected at each deployment site using the isobaric gas-tight samplers, which will also be used to sample fluids from the two focus sites for the shipboard incubations. Finally, we will deployment/recover experimental microbial colonizers to collect microbial biofilms.We have successfully used these devices in previous cruises to collect sufficient biomass for metatranscriptomic analyses.
Dataset Citation
- Cite as: Rolling Deck to Repository (R2R). 2014. 'EM122 Water Column Sonar Data Collected During AT26-10'. NOAA National Centers for Environmental Information. [access date]
Dataset Identifiers
- AT26-10_EM122
- NCEI Metadata ID:gov.noaa.ngdc.mgg.wcd:AT26-10_EM122
ISO 19115-2 Metadata
gov.noaa.ngdc.mgg.wcd:AT26-10_EM122
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Ordering Instructions | Contact the NCEI Water Column Sonar Data Manager to order data. |
Distributor |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Dataset Point of Contact |
NOAA National Centers for Environmental Information ncei.info@noaa.gov |
Dataset Point of Contact | Water Column Sonar Data Manager NOAA National Centers for Environmental Information (303) 497-4742 wcd.info@noaa.gov |
Time Period | 2014-01-15T16:00:21 to 2014-01-20T03:35:18 |
Spatial Reference System | urn:ogc:def:crs:EPSG::4326Vertical Datum: Unknown |
Spatial Bounding Box Coordinates |
West: -104.2929194
East: -102.3409446
South: 9.76427495
North: 12.98260485
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Dataset Progress Status | Complete - production of the data has been completed |
Data Update Frequency | Not planned |
Purpose | Deep-sea hydrothermal vents, first discovered in 1977, are ‘poster child’ ecosystems where microbial chemosynthesis rather than photosynthesis is the primary source of organic carbon. Significant gaps remain in our understanding of the underlying microbiology and biogeochemistry of these fascinating ecosystems. Missing are the identification of specific microorganisms mediating critical reactions in various geothermal systems, metabolic pathways used by the microbes, rates of the catalyzed reactions, amounts of organic carbon being produced, and the larger role of these ecosystems in global biogeochemical cycles. To fill these gaps, we are carrying out an interdisciplinary, international hypothesis-driven research program to understand microbial processes and their quantitative importance at deep-sea vents. Specifically, we will address the following objectives: Determine key relationships between the taxonomic, genetic and functional diversity, as well as the mechanisms of energy and carbon transfer, in deep-sea hydrothermal vent microbial communities. Identify the predominant metabolic pathways and thus the main energy sources driving chemoautotrophic production in high and low temperature diffuse flow vents. Determine energy conservation efficiency and rates of aerobic and anaerobic chemosynthetic primary productivity in high and low temperature diffuse flow vents. Determine gene expression patterns in diffuse-flow vent microbial communities during attachment to substrates and the development of biofilms. |
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International Hydrographic Organization (IHO) Sea Area ("Limits of Oceans and Seas" publication S-23)
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Last Modified: 2020-06-03
For questions about the information on this page, please email: ncei.info@noaa.gov
For questions about the information on this page, please email: ncei.info@noaa.gov