AT26-10 Chemosynthetic Microbial Communities at Deep-Sea Vents (EM122)

The cruise will include 16 dives with DSV Alvin or ROV Jason, to allow time for deployment and collection of experiments and for extensive sampling of discrete vents along the axis of the 9?46?N to 9?53?N segment of the East Pacific Rise (EPR). We propose to deploy experiments/sample a low temperature diffuse flow vent (e.g., Crab Spa; T: 10-30?C), and a higher temperature vent (e.g., L-vent; T: 30-60?C). Furthermore, we will carry out additional opportunistic sampling for a limited set of parameters (e.g., chemistry, 454- pyrotags, FISH, in situ carbon fixation) of vents that fall into these two categories, or that might have developed since the last visit to the site (May 2012). During the course of the cruise we will collect biomass from fluids and biofilms (deployment/recover experimental microbial colonizers) by utilizing a Large Volume Pump (LVP) (McLane WTS 6-1-142LV). We have successfully used this set up in previous cruises and were able to collect up to ~2,000 liters of hydrothermal fluids resulting in enough biomass (cell density is 2-5 x 105 cells per ml) for subsequent ?omic? analyses. During large volume filtration, the volume filtered is recorded by the LVP onboard computer and the vent fluid sampled is monitored with a time series temperature probe, H2S, and pH sensors. Fluid samples for chemical analyses will be collected at each deployment site using the isobaric gas-tight samplers, which will also be used to sample fluids from the two focus sites for the shipboard incubations. Finally, we will deployment/recover experimental microbial colonizers to collect microbial biofilms.We have successfully used these devices in previous cruises to collect sufficient biomass for metatranscriptomic analyses.
  • Cite as: Rolling Deck to Repository (R2R) Program (2013): Water Column Sonar Data Collection (AT26-10, EM122). National Centers for Environmental Information, NOAA. doi:10.7284/114355 [access date]
gov.noaa.ngdc.mgg.wcd:AT26-10_EM122
Search Data
Distribution Formats
  • Kongsberg/Simrad instrument files (.raw or .wcd) (Version: 1)
    • File Specification: Kongsberg/Simrad .raw files are generated by ME70, EK60 or ES60 instruments. They contain power and optionally angular position data with each beam/frequency identified by an independent channel. See the Simrad interface specification manual for further information. The .wcd files are generated by Kongsberg EM302 or EM122 instruments. See Kongsberg documentation for more information.
Ordering Instructions Contact the NCEI Water Column Sonar Data Manager to order data
Distributor Distributor information not available
Dataset Principal Investigator Stefan Sievert
Associate Scientist
Woods Hole Oceanographic Institution
508-289-2305
ssievert@whoi.edu
Time Period 2014-01-15T16:00:21 to 2014-01-20T03:35:18
Spatial Reference System urn:ogc:def:crs:EPSG:4326
Spatial Bounding Box Coordinates
N: 12.98260485
S: 9.76427495
E: -102.3409446
W: -104.2929194
Spatial Coverage Map
Documentation links not available.
Publication Dates
  • creation: 2014-01-15
Dataset Progress Status Complete - production of the data has been completed
Data Update Frequency Not planned
Purpose Deep-sea hydrothermal vents, first discovered in 1977, are ?poster child? ecosystems where microbial chemosynthesis rather than photosynthesis is the primary source of organic carbon. Significant gaps remain in our understanding of the underlying microbiology and biogeochemistry of these fascinating ecosystems. Missing are the identification of specific microorganisms mediating critical reactions in various geothermal systems, metabolic pathways used by the microbes, rates of the catalyzed reactions, amounts of organic carbon being produced, and the larger role of these ecosystems in global biogeochemical cycles. To fill these gaps, we are carrying out an interdisciplinary, international hypothesis-driven research program to understand microbial processes and their quantitative importance at deep-sea vents. Specifically, we will address the following objectives: Determine key relationships between the taxonomic, genetic and functional diversity, as well as the mechanisms of energy and carbon transfer, in deep-sea hydrothermal vent microbial communities. Identify the predominant metabolic pathways and thus the main energy sources driving chemoautotrophic production in high and low temperature diffuse flow vents. Determine energy conservation efficiency and rates of aerobic and anaerobic chemosynthetic primary productivity in high and low temperature diffuse flow vents. Determine gene expression patterns in diffuse-flow vent microbial communities during attachment to substrates and the development of biofilms.
Use Limitations
  • These data are considered raw and have not been subjected to the NOAA's quality control or quality assurance procedures. They are released for limited public use as preliminary data to be used only with appropriate caution. NOAA cannot assume liability for any damages caused by any errors or omissions in the data, nor as a result of the failure of the data to function on a particular system. NOAA makes no warranty, expressed or implied, nor does the fact of distribution constitute such a warranty.
  • Not subject to copyright protection within the United States.
Dataset Citation
  • Cite as: Rolling Deck to Repository (R2R) Program (2013): Water Column Sonar Data Collection (AT26-10, EM122). National Centers for Environmental Information, NOAA. doi:10.7284/114355 [access date]
Custodians
  • DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
Resource Providers
  • University-National Oceanographic Laboratory System (UNOLS)
Sponsors
  • University-National Oceanographic Laboratory System (UNOLS)
Publishers
  • DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
Theme keywords Global Change Master Directory (GCMD) Science Keywords
  • Earth Science > Oceans > Ocean Acoustics > Acoustic Scattering
  • Earth Science > Oceans > Aquatic Sciences > Fisheries
  • Earth Science > Oceans > Ocean Acoustics
  • Earth Science > Biosphere > Ecosystems > Marine Ecosystems > Pelagic
  • Earth Science > Biosphere > Ecosystems > Marine Ecosystems
  • Earth Science > Biosphere > Ecosystems > Marine Ecosystems > Benthic
  • Earth Science > Biosphere > Ecosystems > Aquatic Ecosystems
  • Earth Science > Oceans > Bathymetry/Seafloor Topography > Bathymetry
  • Earth Science > Oceans > Bathymetry/Seafloor Topography > Seafloor Topography
Data Center keywords Global Change Master Directory (GCMD) Data Center Keywords
  • DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
  • DOC/NOAA/NESDIS/NGDC > National Geophysical Data Center, NESDIS, NOAA, U.S. Department of Commerce
Instrument keywords Global Change Master Directory (GCMD) Instrument Keywords
  • In Situ/Laboratory Instruments > Profilers/Sounders > Acoustic Sounders > WCMS > Water Column Mapping System
Place keywords International Hydrographic Organization (IHO) Sea Area ("Limits of Oceans and Seas" publication S-23)
  • North Pacific Ocean
Global Change Master Directory (GCMD) Location Keywords
  • Global
  • Vertical Location > Sea Floor
Use Constraints
  • Cite as: Rolling Deck to Repository (R2R) Program (2013): Water Column Sonar Data Collection (AT26-10, EM122). National Centers for Environmental Information, NOAA. doi:10.7284/114355 [access date]
Access Constraints
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
  • Resource may not be available for up to one year after initial collection.
Fees
  • Free
Lineage information for: collectionHardware
Lineage Statement Unknown
Lineage information for: series
Processor
  • Woods Hole Oceanographic Institution
Acquisition Information (collection)
Instrument
  • Kongsberg EM122
  • SBE 911plus CTD
  • Kongsberg EM122
  • Ocean Surveyor ADCP
Platform
  • Atlantis (AT)
Operation
  • AT26-10_EM122
Last Modified: 2017-05-30
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