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ECOHAB: Kamykowski_D- Florida Process Cruise 1998-11-16 to 1998-11-19 Karenia brevis counts, biochemistry and behavior (NCEI Accession 0000534)


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            title:  ECOHAB: Kamykowski_D- Florida Process Cruise 1998-11-16 to 1998-11-19 Karenia brevis counts, biochemistry and behavior (NCEI Accession 0000534)
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                date:  2001-08-07
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                  Anchor:  https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/person/details/176 Teresa Steely
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                individualName:  Daniel Kamykowski
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                  Anchor:  https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/736 North Carolina State University (NCSU)
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                  Anchor:  https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/736 North Carolina State University (NCSU)
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        abstract:  Water bottle samples were collected from selected depths (variable with station) at 14 stations in the study area. Coulter counts in the 14-28 um size class were routinely determined for comparison with the microscope counts. Water samples were filtered through 40 um Nytex mesh and centrifuged for 10 minutes at 1200 x g to isolate Karenia brevis. Pellets were frozen for subsequent analysis (pg cell-1) of total lipid, neutral lipid, free amino acids, protein and RNA. The biochemical determinations were performed within two weeks of collection at Kamykowski's NCSU laboratory. Aliquots were passed through 0.4 um filters; the filters were placed in 90% acetone for subsequent analysis of chlorophyll and the supernatant was analyzed for nitrate.
        purpose:  To verify that biochemical analyses of dense natural populations of K. brevis can provide interpretable data on cellular biochemical composition. To determine how the field patterns relate to previously determined laboratory patterns of depth-dependent biochemical composition.
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            otherConstraints:  Cite as: Kamykowski, Daniel (2001). ECOHAB: Kamykowski_D- Florida Process Cruise 1998-11-16 to 1998-11-19 Karenia brevis counts, biochemistry and behavior (NCEI Accession 0000534). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0000534. Accessed [date].
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        supplementalInformation:  Product Creation Date: 05/01/2000 ECOHAB (ECology and Oceanography of Harmful Algal Blooms) is a scientific program designed to increase understanding of the fundamental processes underlying the impacts and population dynamics of HABs. This program addresses the many factors at organismal, population, community, and ecosystem levels that determine how HAB species respond to, and potentially alter their environment, the manner in which HAB species affect food-web and community interactions. The program is based on the results of a workshop co-sponsored by National Oceanic and Atmospheric Administration (NOAA) and National Science Foundation (NSF) and currently receives funding from several federal partners including NOAA, NSF, the U.S. Navy and the U.S. Environmental Protection Agency (EPA).
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                    linkage: ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0014/0000534/
                    protocol:  FTP
                    applicationProfile:  Any FTP client
                    name:  FTP
                    description:  These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP).
                    function:  (CI_OnLineFunctionCode) download
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    dataQualityInfo:  (DQ_DataQuality)
        scope:  (DQ_Scope)
            level:  (MD_ScopeCode) dataset
        lineage:  (LI_Lineage)
            processStep:  (LE_ProcessStep)
                description:  NCEI Accession 0000534 v1.1 was published.
                dateTime:
                  DateTime:  2001-08-07T16:07:22Z
                output:  (LE_Source)
                    sourceCitation:  (CI_Citation)
                        title:  NCEI Accession 0000534 v1.1
                        date:  (CI_Date)
                            date: (inapplicable)
                            dateType:  (CI_DateTypeCode) publication
                        citedResponsibleParty:  (CI_ResponsibleParty)
                            individualName: (inapplicable)
                            contactInfo:  (CI_Contact)
                                onlineResource:  (CI_OnlineResource)
                                    linkage: https://www.ncei.noaa.gov/archive/accession/0000534/1.1
                                    protocol:  HTTPS
                                    name:  NCEI Accession 0000534 v1.1
                                    description:  published 2001-08-07T16:07:22Z
                                    function:  (CI_OnLineFunctionCode) download
                            role: (inapplicable)
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    dataQualityInfo:  (DQ_DataQuality)
        scope:  (DQ_Scope)
            level:  (MD_ScopeCode) repository
            levelDescription:  (MD_ScopeDescription)
                other:  NOAA National Centers for Environmental Information
        lineage:  (LI_Lineage)
            processStep:  (LE_ProcessStep)
                description:  NOAA created the National Centers for Environmental Information (NCEI) by merging NOAA's National Climatic Data Center (NCDC), National Geophysical Data Center (NGDC), and National Oceanographic Data Center (NODC), including the National Coastal Data Development Center (NCDDC), per the Consolidated and Further Continuing Appropriations Act, 2015, Public Law 113-235. NCEI launched publicly on April 22, 2015.
                dateTime:
                  DateTime:  2015-04-22T00:00:00
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    metadataMaintenance:  (MD_MaintenanceInformation)
        maintenanceAndUpdateFrequency:  (MD_MaintenanceFrequencyCode) asNeeded
        maintenanceNote:  Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness.
        contact:  (CI_ResponsibleParty)
            organisationName:  NOAA National Centers for Environmental Information
            role:  (CI_RoleCode) custodian
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    acquisitionInformation:  (MI_AcquisitionInformation)
        instrument:  (MI_Instrument)
            identifier:  (MD_Identifier)
                code:  bottle
            type:  bottle
            description:  any type of water bottle sampling device Generic name for water collection device; usually used to determine temperature, salinity and provide water aliquots for measurement of a wide range of parameters; often referred to by a specific type of water sampling bottle, such as a Nansen or Niskin bottle.