ECOHAB: Van Dolah_F- Karenia brevis Cell Cycle Analysis for Determination of In Situ Growth Rates (NCEI Accession 0000538)

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The reported growth rates of Karenia brevis vary from 0.2 to 0.5 divisions per day, both in laboratory and field populations observed. This growth rate alone is not sufficiently high to account for its dominance in the water column. However, careful studies have not been carried out to determine if the documented slow growth rate of K. brevis persists throughout bloom development, or if different rates of growth might characterize bloom initiation, growth, maintenance, and termination phases. For example, it is conceivable that an "explosive" growth stage occurs early during bloom initiation/growth stages that serves to boost the population size, which has not been previously documented. Like most autotrophic dinoflagellates studied, cell division in K. brevis blooms is phased to the diel cycle. Diel phasing of cell division imposes a maximum potential growth rate of 1 division per day in dinoflagellates. The occurrence of an "explosive growth stage" would require the release of K. brevis cells from mechanisms which regulate this circadian rhythm. To address this question, diel phasing of the cell cycle of K. brevis was first documented in laboratory isolates of K. brevis. In situ diel studies were then carried out on naturally occurring blooms of K. brevis during the R/V Pelican cruise (1996) and during ECOHAB process cruises carried out during years 1-5 of ECOHAB Florida (1997-2001). Cell cycle distribution of K. brevis cells and correlation of cell cycle events with vertical migration was determined by sampling at multiple depths (minimum of surface and bottom, depending on depth). For shipboard field studies, whole water samples of K. brevis (1-2 L) were collected every 2 or 3 h throughout a diel cycle, fixed in glutaraldehyde and stored in the dark. Further processing of samples was carried out in the laboratory following completion of the cruise. The flow cytometry method method of Van Dolah and Leighfield was used (1999. J. Phycology 35:1404-1411). Glutaraldehyde-fixed whole water samples were filtered through a 100 micrometer nitex screen to remove large phytoplankton/zooplankton/debris, then filtered by gravity flow through a 10 micrometer nylon screen to collect K. brevis cells. Cells were collected from the screen by rinsing with 2% glutaraldehyde into a 50 ml polypropylene centrifuge tube, then centrifuged at 1000 x g. The cell pellet was then treated with 2 ml of 20 degrees C methanol overnight to remove pigments. Methanol extracted cells were collected by centrifugation (500xg for 3 min) and resuspended in phosphate buffered saline + 0.5% Tween 20 containing 10 micrograms. mL-1 propidium iodide (PI, Sigma, St. Louis, MO) and 10 mg/ml Rnase (Sigma, St. Louis, MO). DNA analysis was carried out on an Epics MXL4 flow cytometer (Coulter, Miami, FL) using a 5 W argon laser with a 488 nm excitation wavelength and 635 nm emission wavelength. Cell aggregates were eliminated by gating all histograms within the linear range using a peak-area cytogram for PI fluorescence. Cell cycle distribution was analyzed using Multicycle software (Phoenix Flow Systems, San Diego, CA).
  • Cite as: Steely, Teresa; US DOC/NOAA/NMFS/Southeast Regional Office/Habitat Conservation Division > Atlantic Branch (2011). ECOHAB: Van Dolah_F- Karenia brevis Cell Cycle Analysis for Determination of In Situ Growth Rates (NCEI Accession 0000538). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://accession.nodc.noaa.gov/0000538. Accessed [date].
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Distributor DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
301-713-3277
NCEI.Info@noaa.gov
Dataset Point of Contact Information Services
DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
301-713-3277
NCEI.Info@noaa.gov
Time Period 1996-09-01 to 1999-09-29
Spatial Bounding Box Coordinates
N: 30.39237
S: 25.44867
E: -81.71588
W: -87.23565
Spatial Coverage Map
General Documentation
Publication Dates
  • publication: 2011-01-06
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Supplemental Information
ECOHAB (ECology and Oceanography of Harmful Algal Blooms) is
a scientific program designed to increase understanding of
the fundamental processes underlying the impacts and
population dynamics of HABs. This program addresses the many
factors at organismal, population, community, and ecosystem
levels that determine how HAB species respond to, and
potentially alter their environment, the manner in which HAB
species affect food-web and community interactions. The
program is based on the results of a workshop co-sponsored
by National Oceanic and Atmospheric Administration (NOAA)
and National Science Foundation (NSF) and currently receives
funding from several federal partners including NOAA, NSF,
the U.S. Navy and the U.S. Environmental Protection Agency
(EPA).
Purpose The research conducted in this portion of the Florida ECOHAB Program will provide insight into which the role of endogenous cellular rhythms in regulating the accumulation growth phase of K. brevis blooms decline by conducting cell cycle studies. This work entails both both laboratory and field experiments. Laboratory studies will be carried out to identify cell cycle regulatory mechanisms in K. brevis, using western blotting to identify cyclins and cyclin dependent kinases in K. brevis, and to determine their relative levels of expression in actively growing versus stationary phase K. brevis populations. In addition we will characterize their expression during the course of the circadian day, as it correlates with cell cycle phase. In situ labeling of K. brevis cells with fluorescently labeled antibodies to cyclins will assist in discerning whether cyclin expression might be suitable for use as a biomarker of actively dividing blooms in the field. Field studies will use flow cytometry to determine in situ growth rates of blooms of K. brevis during the yearly ECOHAB process cruises in order to establish a multiyear record of growth rates in early and late bloom stages.
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  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Steely, Teresa; US DOC/NOAA/NMFS/Southeast Regional Office/Habitat Conservation Division > Atlantic Branch (2011). ECOHAB: Van Dolah_F- Karenia brevis Cell Cycle Analysis for Determination of In Situ Growth Rates (NCEI Accession 0000538). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://accession.nodc.noaa.gov/0000538. Accessed [date].
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Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
Global Change Master Directory (GCMD) Science Keywords
  • EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > PLANKTON > PHYTOPLANKTON
Data Center keywords Global Change Master Directory (GCMD) Data Center Keywords
  • DOC/NOAA/NESDIS/NODC > National Oceanographic Data Center, NESDIS, NOAA, U.S. Department of Commerce
  • DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
  • DOC/NOAA/NMFS > National Marine Fisheries Service, NOAA, U.S. Department of Commerce
NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS
Place keywords NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords
  • OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN
  • OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > GULF OF MEXICO
Project keywords NODC PROJECT NAMES THESAURUS Global Change Master Directory (GCMD) Project Keywords
  • ECOHAB > Ecology and Oceanography of Harmful Algal Bloom
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Steely, Teresa; US DOC/NOAA/NMFS/Southeast Regional Office/Habitat Conservation Division > Atlantic Branch (2011). ECOHAB: Van Dolah_F- Karenia brevis Cell Cycle Analysis for Determination of In Situ Growth Rates (NCEI Accession 0000538). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://accession.nodc.noaa.gov/0000538. Accessed [date].
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
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Lineage information for: dataset
Processing Steps
  • 2011-01-06T04:14:14Z - NCEI Accession 0000538 v1.1 was published.
Output Datasets
Lineage information for: repository
Processing Steps
  • 2015-04-22T00:00:00 - NOAA created the National Centers for Environmental Information (NCEI) by merging NOAA's National Climatic Data Center (NCDC), National Geophysical Data Center (NGDC), and National Oceanographic Data Center (NODC), including the National Coastal Data Development Center (NCDDC), per the Consolidated and Further Continuing Appropriations Act, 2015, Public Law 113-235. NCEI launched publicly on April 22, 2015.
Last Modified: 2019-03-17T18:19:51Z
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