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2023-03-15T01:14:45Z
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
ECOHAB: Doucette_G- Algicidal bacteria and the regulation of Karenia brevis blooms in the Gulf of Mexico from 1998-11-16 to 1999-09-29 (NCEI Accession 0000542)
2014-09-19
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NOAA National Centers for Environmental Information
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0000542
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Teresa Steely
Florida Marine Research Institute (FMRI)
Teresa.Steely@fwc.state.fl.us
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Florida Marine Research Institute (FMRI)
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Doucette, Gregory J.
NOAA National Centers for Coastal Ocean Science
NCCOS.data@noaa.gov
author
Mayali, Xavier
NOAA National Centers for Coastal Ocean Science
NCCOS.data@noaa.gov
author
Mikulski, Tina
NOAA National Centers for Coastal Ocean Science
NCCOS.data@noaa.gov
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US DOC; NOAA; NOS; National Centers for Coastal Ocean Science (NCCOS)
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NOAA Center for Coastal Environmental Health and Biomolecular Research website
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Interactions between bacteria and species of harmful and/or toxic algae are potentially important factors affecting both the population dynamics and toxicity of these algae. Recent reports of bacteria lethal to certain harmful algal bloom (HAB) species, coupled with a rapidly evolving interest in attempting to minimize the adverse effects of HABs through various prevention, control, and mitigation strategies, has focused attention on defining the role of algicidal bacteria in bloom termination. The aim of the present study was to determine if algicidal bacteria active against Karenia brevis, a dinoflagellate responsible for frequent and protracted red tides in the Gulf of Mexico, are present in the waters of the west Florida shelf. To date we have detected one algicidal bacterium from samples obtained during ECOHAB-Florida cruises and we are continuing this effort. A taxon-specific 16S rRNA probe was developed for a previously isolated algicidal strain, 41-DBG2, and we have begun to screen water samples for its presence using fluorescent in-situ hybridization. In addition, we have employed denaturing gradient gel electrophoresis (DGGE) to characterize the diversity of the ambient microbial assemblages and to visualize the band characteristic of strain 41-DBG2. This band can then be analyzed further by excising and sequencing. Our screening results to date have yielded negative results on all but one of the field samples tested, which was positive with the rRNA probe. These largely negative findings may not be unexpected due to the marked temporal and spatial separation of the original algicidal bacterial isolation and the water samples being screened. Future efforts will focus on isolating algicidal bacteria from the same bloom event as the samples for screening are obtained, thereby increasing the likelihood of detecting algicidal strains and permitting a more effective analysis of their population dynamics.
This project dealing with the potential role of algicidal bacteria in regulating K. brevis bloom dynamics is a targeted ECOHAB proposal, the field portion of which is being conducted through a collaborative working relationship with the ECOHAB-Florida regional project. The present dataset reflects results from analyses of samples collected during the ECOHAB-Florida process cruises identified below. The primary use of these samples has been two-fold: first to screen water samples for the presence of a previously identified algicidal bacterium, strain 41-DBG2, using fluorescent in-situ hybridization of a taxon-specific rRNA probe as well as analysis by denaturing gradient gel electrophoresis (DGGE); and, second, to continue attempts at isolating and identifying algicidal bacteria indigenous to the waters of the west Florida shelf.
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0000542
NCEI ACCESSION NUMBER
2001-07-20
publication
bacteria
MICROBIOLOGY
PHYTOPLANKTON
theme
NODC DATA TYPES THESAURUS
bottle
laboratory analysis
instrument
NODC INSTRUMENT TYPES THESAURUS
biological
laboratory analyses
plankton
theme
NODC OBSERVATION TYPES THESAURUS
US DOC; NOAA; NOS; National Center for Coastal Ocean Science; Center for Coastal Environmental Health and Biomolecular Research
US DOC; NOAA; NOS; National Centers for Coastal Ocean Science
dataCentre
NODC COLLECTING INSTITUTION NAMES THESAURUS
Florida Marine Research Institute
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NODC SUBMITTING INSTITUTION NAMES THESAURUS
Ecology and Oceanography of Harmful Algal Blooms (ECOHAB)
project
NODC PROJECT NAMES THESAURUS
Coastal Waters of Florida
Gulf of Mexico
North Atlantic Ocean
place
NODC SEA AREA NAMES THESAURUS
oceanography
theme
WMO_CategoryCode
2012-09-15
publication
DOC/NOAA/NESDIS/NODC > National Oceanographic Data Center, NESDIS, NOAA, U.S. Department of Commerce
DOC/NOAA/NOS/NCCOS > National Centers for Coastal Ocean Science, National Ocean Service, NOAA, U.S. Department of Commerce
DOC/NOAA/NOS/NCCOS/CCEHBR > Center For Coastal Environmental Health and Biomolecular Research, National Centers For Coastal Ocean Science, National Ocean Service, NOAA, U.S. Department Of Commerce
FL/FWC/FMRI > Florida Marine Research Institute, Fish & Wildlife Conservation Commission, Florida
dataCentre
Global Change Master Directory (GCMD) Data Center Keywords
2023
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17
Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)
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Global Change Master Directory (GCMD). 2023. GCMD Keywords, Version 17. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords
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ECOHAB > Ecology and Oceanography of Harmful Algal Bloom
project
Global Change Master Directory (GCMD) Project Keywords
2023
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Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)
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EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA
EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > PROTISTS > PLANKTON > PHYTOPLANKTON
theme
Global Change Master Directory (GCMD) Science Keywords
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Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)
Greenbelt
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OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN
OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > GULF OF MEXICO
place
Global Change Master Directory (GCMD) Location Keywords
2023
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17
Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)
Greenbelt
MD
https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
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Global Change Master Directory (GCMD). 2023. GCMD Keywords, Version 17. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords
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Cite as: Doucette, Gregory J.; Mayali, Xavier; Mikulski, Tina (2014). ECOHAB: Doucette_G- Algicidal bacteria and the regulation of Karenia brevis blooms in the Gulf of Mexico from 1998-11-16 to 1999-09-29 (NCEI Accession 0000542). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0000542. Accessed [date].
Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
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Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
eng; USA
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environment
oceans
biota
-87.23565
-81.71588
25.44867
30.39237
1998-11-16
1999-09-29
ECOHAB (ECology and Oceanography of Harmful Algal Blooms) is a scientific program designed to increase understanding of the fundamental processes underlying the impacts and population dynamics of HABs. This program addresses the many factors at organismal, population, community, and ecosystem levels that determine how HAB species respond to, and potentially alter their environment, the manner in which HAB species affect food-web and community interactions. The program is based on the results of a workshop co-sponsored by National Oceanic and Atmospheric Administration (NOAA) and National Science Foundation (NSF) and currently receives funding from several federal partners including NOAA, NSF, the U.S. Navy and the U.S. Environmental Protection Agency (EPA).
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NCEI Accession 0000542 v1.1 was published.
2014-09-19T17:19:09Z
NCEI Accession 0000542 v1.1
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NCEI Accession 0000542 v1.1
published 2014-09-19T17:19:09Z
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NOAA National Centers for Environmental Information
NOAA created the National Centers for Environmental Information (NCEI) by merging NOAA's National Climatic Data Center (NCDC), National Geophysical Data Center (NGDC), and National Oceanographic Data Center (NODC), including the National Coastal Data Development Center (NCDDC), per the Consolidated and Further Continuing Appropriations Act, 2015, Public Law 113-235. NCEI launched publicly on April 22, 2015.
2015-04-22T00:00:00
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bottle
bottle
any type of water bottle sampling device
Generic name for water collection device; usually used to determine temperature, salinity and provide water aliquots for measurement of a wide range of parameters; often referred to by a specific type of water sampling bottle, such as a Nansen or Niskin bottle.
laboratory analysis
laboratory analysis
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