CHLOROPHYLL A and BACTERIA - BACTERIAL DENSITY ice core data collected in the South Atlantic Ocean and South Pacific Ocean on the LAURENCE M. GOULD and NATHANIEL B. PALMER cruises LMG0106, LMG0205 and others as part of the Southern Ocean GLOBEC project from 2001-07-28 to 2002-09-11 (NCEI Accession 0112364)

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NODC Accession 0112364 includes biological and ice core data collected aboard the LAURENCE M. GOULD and NATHANIEL B. PALMER during cruises LMG0106, LMG0205, NBP0104 and NBP0204 in the South Atlantic Ocean and South Pacific Ocean from 2001-07-28 to 2002-09-11. These data include CHLOROPHYLL A and BACTERIA - BACTERIAL DENSITY. The instruments used to collect these data include ice corer. These data were collected by Christian H. Fritsen of University and Community as part of Southern Ocean GLOBEC. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NODC on 2013-07-23.

The following is the text of the abstract provided by BCO-DMO:

Bacteria Abundance, Biomass and Chlorophyll a in Ice Cores
NOTES:
NBP0104: Cores labelled with "DNA" were collected for DNA analysis.

Contributor:
Dr. Christian Fritsen
University and Community College System of Nevada
Desert Research Institute
Div. of Earth and Ecosystem Sciences
2215 Raggio Parkway
Reno, NV 89512

Office: 775/673-7487

BG 235 - Methods used for chlorophyll a (chla) analysis and bacteria biomass determination
Core Sampling techniques:
Sampling methods for recovery of chlorophyll a and bacteria from sea ice cores follows those described in:
Garrison, D.L. and K.R. Buck(1986), Organism losses during ice melting: a serious bias in sea ice community studies. Polar Biol., 6:237-239.
Recommendations for reporting were used as outlined by:
Horner, R. et al.,(1992), Ecology of Sea Ice Biota. I: Habitat, Terminology and Methodology. Polar Biol. 12:417-427
Analytic Techniques:
Chla (mg m-3):

determined fluorometrically (Turner Designs 10AU Fluorometer) following extraction in 90% acetone (Parsons et al., 1984)
ice core chla corrected to account for chla in filtered sea water (FSW) added to core sections during melting

Bacteria cell abundance (cells m-3) and biomass (mg C m-3):
LMG 0106

preserved (0.5% glutaraldehyde) samples stained with 4',6-diamidino-2-phenylindole (DAPI; 0.1 to 0.3% final concentration), filtered through 0.2 mm black, polycarbonate membrane filters, and mounted onto glass microscope slides on the ship (within 24 hours following collection)
bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus
bacteria biomass determined using cell abundance, cell biovolume (BV; mm3; as determined from mean length and width measurements), and an allometric conversion factor for bacterial carbon per volume specific for DAPI-stained bacteria (cellular carbon = 218 X BV0.86; Loferer-Kribacher et al., 1998).
ice core samples corrected for FSW dilution

NBP 0104

preserved (0.5% glutaraldehyde) samples stained with Sybri Gold (0.01% final concentration), filtered through 0.2 mm Anodisc filters (Whatman), and mounted onto glass microscope slides at home institution (~1-2 months following collection)
bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus
bacteria biomass determined using cell abundance, cell biovolume (BV; mm3), and an allometric conversion factor for bacterial carbon per volume specific for Acridine Orange-stained bacteria (cellular carbon = 89.9 X BV0.59; Simon and Azam, 1989). Note: an AO-specific carbon per volume conversion factor was used in calculating biomass in Sybri Gold-stained samples because both AO and Sybri Gold stain bacteria cells similarly relative to DAPI (unpublished data).
ice core samples corrected for FSW dilution

Loferer-Kribacher, M., Klima, J., and R. Psenner. 1998. Determination of bacterial cell dry mass by transmission electron microscopy and densitometric image analysis. Applied and Environmental Microbiology. 64:688-694.
Parsons,T.R., Maita, Y., and C.M. Lalli. 1984. A manual of chemical and biological methods for seawater analysis. Pergamon Press. Elmsford, New York.
Simon, M., and F. Azam. 1989. Protein content and protein synthesis rates of planktonic marine bacteria. Marine Ecology Progress Series. 51, 201-213.
updated: April 20, 2006
  • Cite as: Fritsen, C.H. (2013). CHLOROPHYLL A and BACTERIA - BACTERIAL DENSITY ice core data collected in the South Atlantic Ocean and South Pacific Ocean on the LAURENCE M. GOULD and NATHANIEL B. PALMER cruises LMG0106, LMG0205 and others as part of the Southern Ocean GLOBEC project from 2001-07-28 to 2002-09-11 (NCEI Accession 0112364). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://accession.nodc.noaa.gov/0112364. Accessed [date].
gov.noaa.nodc:0112364
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Distributor DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
301-713-3277
NCEI.Info@noaa.gov
Dataset Point of Contact Information Services
DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
301-713-3277
NCEI.Info@noaa.gov
Time Period 2001-07-28 to 2002-09-11
Spatial Bounding Box Coordinates
N: -65.62
S: -69.25
E: -65.61
W: -76.781
Spatial Coverage Map
General Documentation
Associated Resources
  • Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
    • NCEI Collection
      Navigate directly to the URL for data access and direct download.
  • bacteria_ice from the SOGLOBEC,Sea Ice Microbes projects of the U.S. GLOBEC,U.S. GLOBEC programs
  • Parent ID (indicates this is related to other data):
    • gov.noaa.nodc:BCO-DMO
Publication Dates
  • publication: 2013-08-23
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Supplemental Information
The Data Originator(s)/Principal Investigator(s) for these data:
Christian H. Fritsen, University and Community

The data were collected during the following cruise(s):
LMG0106
LMG0205
NBP0104
NBP0204

The collection of the data was supported by:
NSF Division of Antarctic Sciences
Purpose This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Fritsen, C.H. (2013). CHLOROPHYLL A and BACTERIA - BACTERIAL DENSITY ice core data collected in the South Atlantic Ocean and South Pacific Ocean on the LAURENCE M. GOULD and NATHANIEL B. PALMER cruises LMG0106, LMG0205 and others as part of the Southern Ocean GLOBEC project from 2001-07-28 to 2002-09-11 (NCEI Accession 0112364). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://accession.nodc.noaa.gov/0112364. Accessed [date].
Cited Authors
  • Fritsen, C.H.
Contributors
Resource Providers
Points of Contact
Publishers
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
BCO-DMO Data Elements Thesaurus
  • bact_abun
  • bact_biomass_C
  • chl_a_ugm
  • core_no
  • cruiseid
  • day_gmt
  • event
  • lat
  • lon
  • month_gmt
  • sect_bot_depth
  • sect_top_depth
  • station
  • year
Global Change Master Directory (GCMD) Science Keywords
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CHLOROPHYLL
Data Center keywords Global Change Master Directory (GCMD) Data Center Keywords
  • DOC/NOAA/NESDIS/NODC > National Oceanographic Data Center, NESDIS, NOAA, U.S. Department of Commerce
  • DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS
Platform keywords NODC PLATFORM NAMES THESAURUS Global Change Master Directory (GCMD) Platform Keywords
  • SHIPS
SeaDataNet Ship and Platform Codes
Instrument keywords NODC INSTRUMENT TYPES THESAURUS Global Change Master Directory (GCMD) Instrument Keywords
  • ICE AUGERS
Place keywords NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords
  • OCEAN > ATLANTIC OCEAN > SOUTH ATLANTIC OCEAN
  • OCEAN > PACIFIC OCEAN > SOUTH PACIFIC OCEAN
Project keywords NODC PROJECT NAMES THESAURUS Global Change Master Directory (GCMD) Project Keywords
  • U.S.GLOBEC-GB > U.S. Global Ocean Ecosystem Dynamics, Georges Bank
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Fritsen, C.H. (2013). CHLOROPHYLL A and BACTERIA - BACTERIAL DENSITY ice core data collected in the South Atlantic Ocean and South Pacific Ocean on the LAURENCE M. GOULD and NATHANIEL B. PALMER cruises LMG0106, LMG0205 and others as part of the Southern Ocean GLOBEC project from 2001-07-28 to 2002-09-11 (NCEI Accession 0112364). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://accession.nodc.noaa.gov/0112364. Accessed [date].
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees
  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2013-08-23T13:46:09Z - NCEI Accession 0112364 v1.1 was published.
Output Datasets
Lineage information for: repository
Processing Steps
  • 2015-04-22T00:00:00 - NOAA created the National Centers for Environmental Information (NCEI) by merging NOAA's National Climatic Data Center (NCDC), National Geophysical Data Center (NGDC), and National Oceanographic Data Center (NODC), including the National Coastal Data Development Center (NCDDC), per the Consolidated and Further Continuing Appropriations Act, 2015, Public Law 113-235. NCEI launched publicly on April 22, 2015.
Acquisition Information (collection)
Instrument
  • ice corer
Platform
  • LAURENCE M. GOULD
  • RV Nathaniel B. Palmer
Last Modified: 2019-05-18T12:45:31Z
For questions about the information on this page, please email: NODC.DataOfficer@noaa.gov