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2024-01-28T14:04:13Z
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Ice core bacteria data from RVIB Nathaniel B. Palmerand ARSV Laurence M. Gould cruises NBP0104, LMG0106, NBP0204,and LMG0205 in the Southern Ocean from 2001-2002 (SOGLOBEC project; Sea Ice Microbes project) (NCEI Accession 0112364)
2013-08-23
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2023-03-30
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0112364
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Chris H. Fritsen
University of Nevada; University of Nevada - Reno; Desert Research Institute (DRI)
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Fritsen, Chris H.
Desert Research Institute
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University of Nevada; University of Nevada - Reno; Desert Research Institute (DRI)
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This dataset contains biological data collected on ARSV Laurence M. Gould and RVIB Nathaniel B. Palmer during cruises LMG0106, LMG0205, NBP0104, and NBP0204 in the South Atlantic Ocean, South Pacific Ocean, and Southern Ocean from 2001-07-28 to 2002-09-07. These data include bacterial abundance, bacterial carbon biomass, and chl_a_ugm. The instruments used to collect these data include Ice Corer. These data were collected by Dr Chris H. Fritsen of Desert Research Institute as part of the "GLOBEC: Sea Ice Microbial Communities (Sea Ice Microbes)" and "U.S. GLOBEC Southern Ocean (SOGLOBEC)" projects and "U.S. GLOBal ocean ECosystems dynamics (U.S. GLOBEC)" program. The Biological and Chemical Oceanography Data Management Office (BCO-DMO) submitted these data to NCEI on 2023-01-23.
The following is the text of the dataset description provided by BCO-DMO:
Ice core bacteria data
Dataset Description:
Bacteria Abundance, Biomass and Chlorophyll a in Ice Cores
NOTES:
NBP0104: Cores labelled with "DNA" were collected for DNA analysis.
Contributor: Dr. Christian Fritsen University and Community College System of Nevada Desert Research Institute Div. of Earth and Ecosystem Sciences 2215 Raggio Parkway Reno, NV 89512
Office: 775/673-7487
BG 235 - Methods used for chlorophyll a (chla) analysis and bacteria biomass determination
Core Sampling techniques:
Sampling methods for recovery of chlorophyll a and bacteria from sea ice cores follows those described in:
Garrison, D.L. and K.R. Buck(1986), Organism losses during ice melting: a serious bias in sea ice community studies. Polar Biol. , 6 :237-239.
Recommendations for reporting were used as outlined by:
Horner, R. et al. ,(1992), Ecology of Sea Ice Biota. I: Habitat, Terminology and Methodology. Polar Biol. 12 :417-427
Analytic Techniques:
Chla (mg m -3 ):
determined fluorometrically (Turner Designs 10AU Fluorometer) following extraction in 90% acetone (Parsons et al ., 1984) ice core chla corrected to account for chla in filtered sea water (FSW) added to core sections during melting
Bacteria cell abundance (cells m -3 ) and biomass (mg C m -3 ):
LMG 0106
preserved (0.5% glutaraldehyde) samples stained with 4',6-diamidino-2-phenylindole (DAPI; 0.1 to 0.3% final concentration), filtered through 0.2 mm black, polycarbonate membrane filters, and mounted onto glass microscope slides on the ship (within 24 hours following collection) bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus bacteria biomass determined using cell abundance, cell biovolume (BV; mm 3 ; as determined from mean length and width measurements), and an allometric conversion factor for bacterial carbon per volume specific for DAPI-stained bacteria (cellular carbon = 218 X BV 0.86 ; Loferer-Krößbacher et al. , 1998). ice core samples corrected for FSW dilution
NBP 0104
preserved (0.5% glutaraldehyde) samples stained with SYBR® Gold (0.01% final concentration), filtered through 0.2 mm Anodisc filters (Whatman), and mounted onto glass microscope slides at home institution (~1-2 months following collection) bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus bacteria biomass determined using cell abundance, cell biovolume (BV; mm 3 ), and an allometric conversion factor for bacterial carbon per volume specific for Acridine Orange-stained bacteria (cellular carbon = 89.9 X BV 0.59 ; Simon and Azam, 1989). Note: an AO-specific carbon per volume conversion factor was used in calculating biomass in SYBR® Gold-stained samples because both AO and SYBR® Gold stain bacteria cells similarly relative to DAPI (unpublished data). ice core samples corrected for FSW dilution
Loferer-Krößbacher, M., Klima, J., and R. Psenner. 1998. Determination of bacterial cell dry mass by transmission electron microscopy and densitometric image analysis. Applied and Environmental Microbiology. 64:688-694.
Parsons,T.R., Maita, Y., and C.M. Lalli. 1984. A manual of chemical and biological methods for seawater analysis. Pergamon Press. Elmsford, New York.
Simon, M., and F. Azam. 1989. Protein content and protein synthesis rates of planktonic marine bacteria. Marine Ecology Progress Series. 51, 201-213.
updated: April 20, 2006
This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Funding provided by NSF Antarctic Sciences (NSF ANT) Award Number: ANT-9910098 Award URL: http://www.nsf.gov/awardsearch/showAward.do?AwardNumber=9910098
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0112364
NCEI ACCESSION NUMBER
2023-01-23
publication
bacteria
BACTERIA - BACTERIAL DENSITY
CHLOROPHYLL A
SPONGE SPECIES IDENTIFICATION
theme
NODC DATA TYPES THESAURUS
ice corer
instrument
NODC INSTRUMENT TYPES THESAURUS
biological
theme
NODC OBSERVATION TYPES THESAURUS
R/V Laurence M. Gould
RV Nathaniel B. Palmer
platform
NODC PLATFORM NAMES THESAURUS
University of Nevada; University of Nevada - Reno; Desert Research Institute
dataCentre
NODC COLLECTING INSTITUTION NAMES THESAURUS
Biological and Chemical Oceanography Data Management Office
dataCentre
NODC SUBMITTING INSTITUTION NAMES THESAURUS
GLOBal Ocean ECosystems Dynamics (US GLOBEC)
Southern Ocean GLOBEC (SO-GLOBEC)
project
NODC PROJECT NAMES THESAURUS
South Atlantic Ocean
South Pacific Ocean
Southern Ocean
place
NODC SEA AREA NAMES THESAURUS
oceanography
theme
WMO_CategoryCode
2012-09-15
publication
BCO-DMO > Biological and Chemical Oceanography Data Management Office
DOC/NOAA/NESDIS/NODC > National Oceanographic Data Center, NESDIS, NOAA, U.S. Department of Commerce
DRI > Desert Research Institute
dataCentre
Global Change Master Directory (GCMD) Data Center Keywords
2023
revision
17
Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)
Greenbelt
MD
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GCMD Keyword Forum Page
Global Change Master Directory (GCMD). 2023. GCMD Keywords, Version 17. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords
information
custodian
U.S. GLOBal ocean ECosystems dynamics (U.S. GLOBEC)
project
BCO-DMO Standard Programs
GLOBEC: Sea Ice Microbial Communities (Sea Ice Microbes)
U.S. GLOBEC Southern Ocean (SOGLOBEC)
project
BCO-DMO Standard Projects
GLOBEC > Global Ocean Ecosystem Dynamics, IGBP
U.S.GLOBEC-SO > U.S. Global Ocean Ecosystem Dynamics, Southern Ocean
project
Global Change Master Directory (GCMD) Project Keywords
2023
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Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)
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information
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LMG0106
LMG0205
NBP0104
NBP0204
project
Provider Cruise IDs
Funding provided by NSF Antarctic Sciences (NSF ANT) Award Number: ANT-9910098
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Provider Funding Award Information
bacterial abundance
bacterial carbon biomass
chl_a_ugm
core id
cruise id
day_gmt
event
latitude
longitude
month_gmt
no standard parameter
station
year
yrday_gmt
theme
BCO-DMO Standard Parameters
EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > SPONGES
EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > BACTERIA/ARCHAEA
EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CHLOROPHYLL
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bact_abun
bact_biomass_C
chl_a_ugm
core_no
cruiseid
day_gmt
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lat
lon
month_gmt
sect_bot_depth
sect_top_depth
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Ice Corer
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ICE AUGERS
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IceCoring
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Originator Instrument Names
ARSV Laurence M. Gould
RVIB Nathaniel B. Palmer
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BCO-DMO Platform Names
Ships
platform
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LAURENCE M. GOULD (call sign: WCX7445, ICES code: 33LG, 1998)
NATHANIEL B. PALMER (call sign: WPB3210, ICES code: 3206, 1992)
platform
ICES/SeaDataNet Ship Codes
OCEAN > ATLANTIC OCEAN > SOUTH ATLANTIC OCEAN
OCEAN > PACIFIC OCEAN > SOUTH PACIFIC OCEAN
OCEAN > SOUTHERN OCEAN
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Southern Ocean
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accessLevel: Public
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Cite as: Fritsen, Chris H. (2013). Ice core bacteria data from RVIB Nathaniel B. Palmerand ARSV Laurence M. Gould cruises NBP0104, LMG0106, NBP0204,and LMG0205 in the Southern Ocean from 2001-2002 (SOGLOBEC project; Sea Ice Microbes project) (NCEI Accession 0112364). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://www.ncei.noaa.gov/archive/accession/0112364. Accessed [date].
Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
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This dataset is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License.
SPDX License: Creative Commons Attribution 4.0 International (CC-BY-4.0)
Biological, chemical, physical, biogeochemical, ecological, environmental and other data collected from around the world during historical and contemporary periods of biological and chemical oceanographic exploration and research managed and submitted by the Biological and Chemical Oceanography Data Management Office (BCO-DMO)
2009-12-01
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Fritsen, C. (2003) Ice core bacteria data from RVIB Nathaniel B. Palmerand ARSV Laurence M. Gould cruises NBP0104, LMG0106, NBP0204,and LMG0205 in the Southern Ocean from 2001-2002 (SOGLOBEC project; Sea Ice Microbes project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). Dataset version 2003-12-03. https://doi.org/10.1575/1912/bco-dmo.2348.1
2003-12-03
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Chris H. Fritsen
University of Nevada; University of Nevada - Reno; Desert Research Institute (DRI)
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Desert Research Institute
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eng; USA
utf8
environment
oceans
biota
-76.781
-65.61
-69.25
-65.62
2001-07-28
2002-09-07
Acquisition Description:
Sampling methods for recovery of chlorophyll a and bacteria from sea ice cores follows those described in:
Garrison, D.L. and K.R. Buck(1986), Organism losses during ice melting: a serious bias in sea ice community studies. Polar Biol. , 6 :237-239.
Recommendations for reporting were used as outlined by:
Horner, R. et al. ,(1992), Ecology of Sea Ice Biota. I: Habitat, Terminology and Methodology. Polar Biol. 12 :417-427
# Ice core bacteria data from Southern Ocean GLOBEC # C. Fritsen and F. Stewart # * BactAbun = bacterial abundance = cells m-3 # * BactBio = bacterial biomass = mg C m-3 = ug C l-1 # * chla = mg chla m-3 = ug chla l-1 # * sect_top_depth = depth(m) at top of core section
In this accession, NCEI has archived multiple versions of these data. The latest (and best) version of these data has the largest version number.
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NCEI Accession 0112364 v1.1 was published.
2013-08-23T13:46:08Z
NCEI Accession 0112364 v1.1
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NCEI Accession 0112364 v1.1
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NCEI Accession 0112364 was revised and v2.2 was published.
Updates were received for this dataset. These updates were copied into the data/0-data/ directory of this accession. These updates may provide additional files or replace obsolete files. This version contains the most complete and up-to-date representation of this archival information package. All of the files received prior to this update are available in the preceding version of this accession.
2023-03-30T17:31:48Z
NCEI Accession 0112364 v2.2
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2015-04-22T00:00:00
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Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness.
NCEI Accession 0112364 was revised and a new version of the archival package was published. Updates to existing archival packages may provide additional files or replace obsolete files. The latest version contains the most complete and up-to-date representation of this archival information package. All of the files received prior to this update are available in the preceding version of this accession. Please see journal.txt in the /about directory for additional details on changes made.
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ice corer
ice corer
An ice corer is used to drill into deep ice and remove long cylinders of ice from which information about the past and present can be inferred.
R/V Laurence M. Gould
Flag: United States of America
Vessel Type: Research Vessel; Ice Breaker
DOB: Dec 1997
IMO: 9137337
The RV Laurence M. Gould is an icebreaker used by researchers from the United States' National Science Foundation for research in the Southern Ocean. The vessel is named after Laurence McKinley Gould a widely recognized American scientist, who had explored both the Arctic and Antarctic. She was built in Louisiana to replace the RV Polar Duke.
RV Nathaniel B. Palmer
Updated information from ICES for the vessel NATHANIEL B. PALMER from UNITED STATES.
Use 3206 for all data attributed to the vessel NATHANIEL B. PALMER from UNITED STATES.
This platform has a current Call Sign = WPB3210 and two previous call signs, related to owner/company name changes since 1992. Previous call signs for NATHANIEL B. PALMER are:
KUS1475 (1992 - ca. 1996)
WCE9345 (ca. 1996 - mid-2006)
WBP3210 (mid-2006 to present)
Name : NATHANIEL B. PALMER
Flag : UNITED STATES - US (ISO Country Code)
Call Sign : WBP3210
Title : RV
Platform Class : 31 Research vessel
IMO : 9007257
Commissioned Date : 1992
Current Length : 86
Built : 1992-03
Notes : Data also found under previous call sign KUS1475
Lloyds URL : http://www.seasearcher.com/lmiu/vessels/overview.htm?vesselID=241527
Confirmed by NOAA on 2013-11-19.