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abstract: This dataset contains laboratory experiment data that were collected to examine the effects of elevated levels of CO2 on phytoplankton physiology and nutrition for fishery-based food webs. Phytoplankton are single-celled photosynthetic organisms at the base of marine food webs that support finfish and shellfish production. At present, it is unclear how changes in atmospheric partial pressure of CO2 and ocean pH will affect phytoplankton physiology and community structure. We were funded to begin single-species, laboratory-culture experiments assessing the influence of experimentally-varied steady-state pH/CO2 upon phytoplankton physiology and nutritional content, including growth rate, elemental composition (C, N, P), total carbohydrates, lipids, and fatty acids. The data presented here represent the single species experiments done from 2011-2013.
purpose: To do laboratory manipulation to look at the effects of elevated pCO2 on specific growth rate, C:N:P ratios, total lipid and lipid class, and fatty acid composition in single species experiments of many different types of phytoplankton.
credit: Funding Information: NOAA Ocean Acidification Program (Effects of ocean acidification on phytoplankton physiology and nutrition for fishery-based food webs, OAPFY13.03.NEFSC.001)
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keyword: Phospholipids
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keyword: pH
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beginPosition: 2011-05-31
endPosition: 2013-02-07
supplementalInformation: PRINCIPAL INVESTIGATORS: King, Andrew; Meseck, Shannon L.; Milke, Lisa; Wifkors, Gary; and Jenkins, Bethany. FUNDING AGENCY: NOAA's Ocean Acidification Program (OAP). PROJECT TITLE: Effects of ocean acidification on phytoplankton physiology and nutrition for fishery-based food webs. PROJECT ID: OAPFY13.03.NEFSC.001. In this accession, NCEI has archived multiple versions of these data. The latest (and best) version of these data has the largest version number.
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orderingInstructions: Contact NCEI for other distribution options and instructions.
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dataQualityInfo: (DQ_DataQuality)
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description: Parameter or Variable: Dissolved Inorganic Carbon; Abbreviation: DIC; Unit: umol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing; Analyzing instrument: Infra-red detector; Detailed sampling and analyzing information: Apollo SciTech's DIC AS-C3, Bogart, GA, USA, small volumes less than 1 mL, precission plus minus 2 umol/kg; Standardization description: Used CRM to ensure accuracy of instrument during sampling; Standardization frequency: every 3 hours during anlaysis; CRM manufacturer: Andrew Dickson's lab at Scripps Institute of Oceanography; Preservation method: None ran within 5 minutes of collecting sample; Preservative volume: None; Preservative correction: no correction.; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Total alkalinity; Abbreviation: TA; Unit: umol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing; Analyzing instrument: Alkalinity titrator, pH electrode ws Model 8104BNUWP Ross ultra glass combination pH electrod with rugged bulb. Electrode was calibrated using a trise base buffer at pH 8.094 from Andrew Dickson Batch 7, Bottle 102; Cell type (open or closed): Open; CRM manufacturer: Andrew Dickson's lab at Scripps Institute of Oceanography; CRM batch number: CRM 112 and CRM 98; Preservation method: None; Preservative volume: None; Preservative correction: no correction.; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: pH; Abbreviation: pH; pH scale: Total; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing; Analyzing instrument: Varian Cary 100 Spectrometer; Temperature of pH measurement: 20 C; Detailed sampling and analyzing information: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing was used to fill 10 mm cells. pH measurements were taken at 20C and recorded at 20C. Dye batch number was lot number 1080-0253; Temperature of standardization: 20; Temperature correction method: none; At what temperature was pH reported: 20; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Date of water collection; Abbreviation: Collection Date; Detailed sampling and analyzing information: This is the date that the water sample was collected from the coordinates above, Water was filtered to 0.22 um and stored in the cold (4C) until utalized for experiments; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Date of experiment; Abbreviation: Date; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Species; Abbreviation: Species; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Light Intensity; Abbreviation: Light; Unit: umoles m2s-1; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulated condition; Measured or calculated: Measured; Analyzing instrument: Li-Cor, Inc. Model LI-185B Quantum photometer; Replicate information: triplicate measurements along the light source, light was on for 14 hours per day; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Salinity; Abbreviation: S; Controlled vocabulary name: Salinity; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Sampling instrument: Glass pipette; Analyzing instrument: Refractometer Atago; Detailed sampling and analyzing information: refractometer was zerod with Milli-Q water at 18 ohms. Once zeroed refractometer was cleaned and a drop of seawater was put on it and measurments were taken.; Replicate information: triplicate measurements; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: dissolved silicate; Abbreviation: Si; Unit: umol/Kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: measured; Analyzing instrument: Quattro, Test range0-20umol/L, 10 mm flowcell with LED photometer at 820 nm, 60 samples/hr, standard deviation is 0.05 umol/L, detetion limit is 0.01 umol/L; Detailed sampling and analyzing information: Samples were taken in 250 ml amber polypropylene bottles and ran immediately after collection; Method reference: Method NO Q-066-05 Rev. 3 for QuAAtro Applications developed by the Royal Netherlands Institute for Sea Research; Researcher name: Shannon Meseck; Researcher institution: National Marine Fisheries Service.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Dissolved Phosphate; Abbreviation: P; Unit: umol/Kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Analyzing instrument: Quattro, Test range 0-4 umol/L, 10 mm flowcell with LED photometer at 880 nm, 60 samples/hr, standard deviation is 0.01 umol/L, detetion limit is 0.004umol/L; Detailed sampling and analyzing information: Samples were taken in 250 ml amber polypropylene bottles and ran immediately after collection; Method reference: Method NO Q-064-05 Rev. 3 for QuAAtro Applications developed by the Royal Netherlands Institute for Sea Research; Researcher name: Shannon Meseck; Researcher institution: National Marine Fisheries Service.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Dissolved nitrate+nitrite; Abbreviation: N; Unit: umol/Kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulated condition; Measured or calculated: Measured; Analyzing instrument: Quattro, Test range 0-250 umol/L, 10 mm flowcell with LED photometer at 520nm, 90 samples/hr, standard deviation is 0.03 umol/L, detetion limit is 0.02 umol/L; Detailed sampling and analyzing information: Samples were taken in 250 ml amber polypropylene bottles and ran immediately after collection; Method reference: Method NO Q-068-05 Rev. 4 for QuAAtro Applications developed by the Royal Netherlands Institute for Sea Research; Researcher name: Shannon Meseck; Researcher institution: National Marine Fisheries Service.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Alkalinity Corrected; Abbreviation: AC; Unit: umol/ kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: measured; Analyzing instrument: Alkalinity titrator; Detailed sampling and analyzing information: Known CRM were used with our alkalinity titrator. The differences between our measured alkalinity and the reported alkalinity is recorded in this column.; Replicate information: Triplicates; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: DIC Corrected; Abbreviation: DIC Cor; Unit: umol/ kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: measured; Analyzing instrument: Infra-red detector; Detailed sampling and analyzing information: Apollo SciTech's DIC AS-C3, Bogart, GA, USA, small volumes less than 1 mL, precission plus minus 2 umol/kg; Replicate information: Triplicates; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: CRM adjusted alkalinity; Abbreviation: CRM adj alk; Unit: umol/ kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: measured; Analyzing instrument: Alkalinity titrator; Detailed sampling and analyzing information: Known CRM were used with our alkalinity titrator. The differences between our measured alkalinity and the reported alkalinity is corrected in this column by how much the CRM was off.; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: CRM adjusted Dissolved Inorganic Carbon; Abbreviation: CRM adj DIC; Unit: umol/ kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: measured; Analyzing instrument: Infra-red detector; Detailed sampling and analyzing information: Apollo SciTech's DIC AS-C3, Bogart, GA, USA, small volumes less than 1 mL, precission plus minus 2 umol/kg; Quality flag convention: This is the correct data based on CRM values that we obtained with the Apollo SciTech instrument. We corrected our measurements accordingly.; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Alkalnity pH calculated pCO2 from CO2SYS; Abbreviation: Alk-pH pCO2; Unit: ppm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Calculated; Detailed sampling and analyzing information: CO2SYS; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Alkalnity DIC calculated pCO2 from CO2SYS; Abbreviation: Alk-DIC pCO2; Unit: ppm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Calculated; Detailed sampling and analyzing information: CO2SYS; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: DIC pH calculated pCO2 from CO2SYS; Abbreviation: DIC-pH pCO2; Unit: ppm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Calculated; Detailed sampling and analyzing information: CO2SYS; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: division rate; Abbreviation: u; Unit: d-1; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: measured; Detailed sampling and analyzing information: growth rate are calculted by the number of cells difference from the intial experiment to the end of the experiment; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: particulate nitrogen; Abbreviation: Part N; Unit: umol/ l; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 250 ml amber bottles.; Analyzing instrument: Costech ECS 4010 elemental analyzer; Duration: experiment studied; Detailed sampling and analyzing information: Samples were taken in 250 ml amber polypropylene bottles and filtered on 500C combusted GF/F filters. Filters where then dried, rolled and ran on the elemental analyzer.; Replicate information: Doublicate samples were taken from each culture; Biological subject: Phytoplankton; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: particulate carbon; Abbreviation: Part C; Unit: umol/ l; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 250 ml amber bottles.; Analyzing instrument: Costech ECS 4010 elemental analyzer; Detailed sampling and analyzing information: Samples were taken in 250 ml amber polypropylene bottles and filtered on 500C combusted GF/F filters. Filters where then dried, rolled and ran on the elemental analyzer.; Replicate information: Doublicate samples were taken from each culture; Biological subject: Phytoplankton; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: particulate phosphate; Abbreviation: Part P; Unit: umol/ l; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 250 ml amber bottles.; Analyzing instrument: Costech ECS 4010 elemental analyzer; Detailed sampling and analyzing information: Samples were taken in 250 ml amber polypropylene bottles and filtered on 500C combusted GF/F filters. Filters where then dried, rolled and ran on the elemental analyzer.; Method reference: Fu, F., et al. "The biological and biogeochemical consequences of phosphate scavenging onto phytoplankton cell surfaces." Limnology and oceanography 50.5 (2005): 1459.; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: biogenic silica; Abbreviation: Part Si; Unit: umol/ l; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 250 ml amber bottles.; Detailed sampling and analyzing information: Samples were taken in 250 ml amber polypropylene bottles and filtered on; Method reference: Nelson, David M., Treguer, Paul, Brzezinski, Mark A., Leynaert, Aude, Queguiner, Bernard. 1995. Production and dissolution of biogenic silica in the ocean: Revised global estimates, comparison with regional data and relationship to biogenic sedimentation Global Biogeochem. Cycles 9:359-372.; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Final cell counts; Abbreviation: Final cells; Unit: cells/ ml; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 250 ml amber bottles.; Analyzing instrument: Fascan flow cytometer; Detailed sampling and analyzing information: 1 ml samples were taken and ran on a flow cytometer to get final cell counts using FL-3 fluorescence and side scatter; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: hydrocarbons; Abbreviation: HC; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Iatroscan Model number MK-6s; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Kenneth Hammel; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: steryl esters/wax esters; Abbreviation: SE/WE; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Iatroscan Model number MK-6s; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Kenneth Hammel; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: ethyl esters; Abbreviation: EE; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Iatroscan Model number MK-6s; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Kenneth Hammel; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: methyl esters; Abbreviation: ME; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Iatroscan Model number MK-6s; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Kenneth Hammel; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: ethyl ketones; Abbreviation: EK; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Iatroscan Model number MK-6s; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Kenneth Hammel; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: methyl ketones; Abbreviation: MK; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Iatroscan Model number MK-6s; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Kenneth Hammel; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: gylcerol ethers; Abbreviation: GE; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Iatroscan Model number MK-6s; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Kenneth Hammel; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Triacylglycerols; Abbreviation: Triacylglycerols; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Iatroscan Model number MK-6s; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Kenneth Hammel; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: free fatty acids; Abbreviation: FFA; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Iatroscan Model number MK-6s; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Kenneth Hammel; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Alcohols; Abbreviation: Alcohols; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Iatroscan Model number MK-6s; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Kenneth Hammel; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Sterols; Abbreviation: Sterols; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Iatroscan Model number MK-6s; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Kenneth Hammel; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: diacylglycerols; Abbreviation: diacylglycerols; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Iatroscan Model number MK-6s; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Kenneth Hammel; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: acetone mobile polar lipids; Abbreviation: AMPL; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Iatroscan Model number MK-6s; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Kenneth Hammel; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Phospholipids; Abbreviation: Phospholipids; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Iatroscan Model number MK-6s; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Kenneth Hammel; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 14:0; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: trimethyltrideconoic acid; Abbreviation: TMTD; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 14:1; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: i15:0; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: ai15:0; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 15:0; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 15:1; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: i16:0; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: ai16:0?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: pristanic?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 16:0; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 16:1w11?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 16:1w9?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 16:1w7; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 16:1w5; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: i17:0; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: ai17:0; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 16:2w4; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: phytanic?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 17:0; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 16:3w4?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 17:1; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 16:3w3?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 16:4w3?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 16:4w1; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 18:0; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 18:1w11?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 18:1w9; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 18:1w7; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 18:1w6?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 18:1w5?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 18:2a; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 18:2b; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 18:2w6; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 18:2w4; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 18:3w6; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 19:0; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 18:3w4; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 18:3w3; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 18:4w3; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 18:4w1?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 20:0; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 18:5w3; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 20:1w11?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 20:1w9; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 20:1w7?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 20:2a?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 20:2b?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 20:2w6; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 20:3w6; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 21:0; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 20:4w6; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 20:3w3; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 20:4w3; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 20:5w3; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 22:0; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 22:1w11(13); Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 22:1w9; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 22:1w7; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 22:2NIMDa?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 22:2NIMDb?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 21:5w3?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 23:0; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 22:4w6?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 22:5w6; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 22:4w3?; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 22:5w3; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 24:0; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 22:6w3; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Fatty acids; Abbreviation: 24:1; Unit: % of total fatty acids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles.; Analyzing instrument: Gas Chromotagraph; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned.; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Phytoplankton; Researcher name: Andrew King; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration .
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dataQualityInfo: (DQ_DataQuality)
scope: (DQ_Scope)
level: (MD_ScopeCode) repository
levelDescription: (MD_ScopeDescription)
other: NOAA National Centers for Environmental Information
lineage: (LI_Lineage)
processStep: (LE_ProcessStep)
description: NOAA created the National Centers for Environmental Information (NCEI) by merging NOAA's National Climatic Data Center (NCDC), National Geophysical Data Center (NGDC), and National Oceanographic Data Center (NODC), including the National Coastal Data Development Center (NCDDC), per the Consolidated and Further Continuing Appropriations Act, 2015, Public Law 113-235. NCEI launched publicly on April 22, 2015.
dateTime:
DateTime: 2015-04-22T00:00:00
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metadataMaintenance: (MD_MaintenanceInformation)
maintenanceAndUpdateFrequency: (MD_MaintenanceFrequencyCode) asNeeded
maintenanceNote: Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness.
maintenanceNote: NCEI Accession 0121255 was revised and a new version of the archival package was published. Updates to existing archival packages may provide additional files or replace obsolete files. The latest version contains the most complete and up-to-date representation of this archival information package. All of the files received prior to this update are available in the preceding version of this accession. Please see journal.txt in the /about directory for additional details on changes made.
contact: (CI_ResponsibleParty)
organisationName: NOAA National Centers for Environmental Information
role: (CI_RoleCode) custodian
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acquisitionInformation: (MI_AcquisitionInformation)
instrument: (MI_Instrument)
identifier: (MD_Identifier)
code: spectrophotometer
type: spectrophotometer
description: spectrophotometer, optical spectrometer, spectrograph or spectroscope An optical spectrometer (spectrophotometer, spectrograph or spectroscope) is an instrument used to measure properties of light over a specific portion of the electromagnetic spectrum, typically used in spectroscopic analysis to identify materials. Butler, L. R. P.; Laqua, K. (1995). "Nomenclature, symbols, units and their usage in spectrochemical analysis-IX. Instrumentation for the spectral dispersion and isolation of optical radiation (IUPAC Recommendations 1995)". Pure Appl. Chem. IUPAC. 67 (10): 1725–1744. doi:10.1351/pac199567101725. A spectrometer is the general term for describing a combination of spectral apparatus with one or more detectors to measure the intensity of one or more spectral bands."
