The impact of ocean acidification on the early life stages of surf clams and the interactive effects of feeding and temperature from laboratory experiment studies from 2011-07-12 to 2012-06-17 (NODC Accession 0123314)

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This archival package contains laboratory experiment data that were collected to examine the effects of ocean acidification on the Atlantic surfclam, Spisula solidissima, a species worth $31 million in 2009. Ocean acidification has negatively impacted growth and survival of multiple bivalve species, but because each species and developmental stage can show different responses, these studies were designed to determine potential impacts of increased CO2 on the larvae of the commercially important surfclam. Additionally, the role of nutrition (i.e. phytoplankton concentration) was included in a portion of these experiments because food availability may be able to mitigate the stress of ocean acidification and because ocean acidification has the potential to impact marine phytoplankton communities. During the summer of 2011, three different experiments were conducted at Woods Hole Oceanographic Institution examining the effects of three different pCO2 concentrations on larval surf clams. Two short term experiments (~70h) examined the effect of food availability on early shell development (fed vs unfed). One long term experiment (~21d) was conducted to examine the effects of pCO2 on shell development and metamorphic success (all animals well fed). Carbonate data is reported from these preliminary short-term experiments, and survival and shell length data is reported, in addition to carbonate data, from the long-term experiment. During 2012, one 6 day experiment was conducted examining the role and potential interactive effects of high and low food availability (400 and 40,000 cells ml-1 Tiso) and differential CO2 concentrations (ambient, ~1200 ppm and ~2200ppm). From these experiments, carbonate data, shell length, mass and biochemical compositions are reported. In 2013, two additional experiments were conducted to confirm results obtained in 2012. Unfortunately we observed stunted larval growth, no feeding effect on growth, high mortalities and a general failure to thrive. Given this, we infer poor gamete quality may have been the cause, and have chosen not to interpret these data as results are suspect. Therefore, 2013 data are therefore not included in this data submission.
  • Cite as: Milke, Lisa M.; McCorkle, Daniel; Cohen, Anne; Widman, James C., Jr. (2014). The impact of ocean acidification on the early life stages of surf clams and the interactive effects of feeding and temperature from laboratory experiment studies from 2011-07-12 to 2012-06-17 (NODC Accession 0123314). Version 1.1. National Oceanographic Data Center, NOAA. Dataset. doi:10.7289/V56H4FCZ [access date]
gov.noaa.nodc:0123314
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Distributor DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
301-713-3277
NCEI.Info@noaa.gov
Dataset Point of Contact Information Services
DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
301-713-3277
NCEI.Info@noaa.gov
Time Period 2011-07-12 to 2012-06-17
Spatial Bounding Box Coordinates
N: 41.53
S: 41.53
E: -70.64
W: -70.64
Spatial Coverage Map
General Documentation
  • Project Metadata
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  • Dataset Description
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Publication Dates
  • publication: 2014-12-03
Edition 1.1
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Data Update Frequency As needed
Supplemental Information
PRINCIPAL INVESTIGATORS: Milke, Lisa; McCorkle, Daniel; Cohen, Anne; Widman, James.
FUNDING AGENCY: NOAA's Ocean Acidification Program (OAP).
PROJECT TITLE: An experimental investigation of the impact of ocean acidification on the early life stages of surf clams and sea scallops, and the interactive effects of feeding and temperature.
PROJECT ID: OAPFY13.03.NEFSC.002.
Purpose To determine whether varying levels of PCO2 have any effect on length, weight and survival of larval surfclams.
Use Limitations
  • accessLevel: Public
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Dataset Citation
  • Cite as: Milke, Lisa M.; McCorkle, Daniel; Cohen, Anne; Widman, James C., Jr. (2014). The impact of ocean acidification on the early life stages of surf clams and the interactive effects of feeding and temperature from laboratory experiment studies from 2011-07-12 to 2012-06-17 (NODC Accession 0123314). Version 1.1. National Oceanographic Data Center, NOAA. Dataset. doi:10.7289/V56H4FCZ [access date]
Cited Authors
  • Milke, Lisa M.
  • McCorkle, Daniel
  • Cohen, Anne
  • Widman, James C., Jr.
Principal Investigators
Contributors
Resource Providers
Publishers
Acknowledgments
  • Funding Information: NOAA's Ocean Acidification Program (An experimental investigation of the impact of ocean acidification on the early life stages of surf clams and sea scallops, and the interactive effects of feeding and temperature, OAPFY13.03.NEFSC.002)
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
Global Change Master Directory (GCMD) Science and Services Keywords
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ALKALINITY
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBONATE
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > HYDROCARBONS
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > INORGANIC CARBON
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > NITRATE
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > NITRITE
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PHOSPHATE
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > SILICATE
  • EARTH SCIENCE > OCEANS > OCEAN TEMPERATURE > WATER TEMPERATURE
  • EARTH SCIENCE > OCEANS > SALINITY/DENSITY > SALINITY
Provider Variable Names
  • AL
  • AMPL
  • Aragonite Saturation State
  • DAG
  • DIC
  • Dead
  • EE
  • EK
  • FFA
  • GE
  • HC
  • Live
  • ME
  • MK
  • Mean Shell length
  • Mean weight of 50 animals
  • NH4
  • NO2
  • NO3
  • NO3+NO2
  • PL
  • PO4
  • SE/WE
  • ST
  • Salinity
  • Si
  • TA
  • TAG
  • Temperature
  • pCO2
  • pH
Data Center keywords Global Change Master Directory (GCMD) Data Center Keywords
  • DOC/NOAA/NESDIS/NODC > National Oceanographic Data Center, NESDIS, NOAA, U.S. Department of Commerce
  • DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
  • DOC/NOAA/NMFS/NEFSC > Northeast Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce
  • WHOI > WOODS HOLE OCEANOGRAPHIC INSTITUTION
NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS
Instrument keywords NODC INSTRUMENT TYPES THESAURUS Global Change Master Directory (GCMD) Instrument Keywords
  • OPTSPEC > Optical Spectrometer
Place keywords NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords
  • OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN
Provider Geographic Names
  • North Atlantic Ocean off the coast of Woods Hole, MA
Project keywords NODC PROJECT NAMES THESAURUS Ocean Acidification Search Keywords
  • Ocean Acidification Data Stewardship (OADS) Project
  • Ocean Acidification Program (OAP)
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Milke, Lisa M.; McCorkle, Daniel; Cohen, Anne; Widman, James C., Jr. (2014). The impact of ocean acidification on the early life stages of surf clams and the interactive effects of feeding and temperature from laboratory experiment studies from 2011-07-12 to 2012-06-17 (NODC Accession 0123314). Version 1.1. National Oceanographic Data Center, NOAA. Dataset. doi:10.7289/V56H4FCZ [access date]
Access Constraints
  • NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
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Lineage information for: dataset
Processing Steps
  • 2014-12-03T21:22:11 - NCEI Accession 0123314 v1.1 was published.
Output Datasets
Lineage information for: dataset
Processing Steps
  • Parameter or Variable: Dissolved Inorganic Carbon; Abbreviation: DIC; Unit: micromol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: measured in 2011, calculated from pH and alk in 2012; Sampling instrument: syringe, 0.45 um filter; Analyzing instrument: vacuum extraction line with manometer; Detailed sampling and analyzing information: water sample sealed in pre-poisoned glass ampuole with N2 in headspace; sample ampoule cracked, acidified, and stripped on vacuum line; CO2 purifiec cryogenically and measured manometrically.; Replicate information: replicate buckets, no replicate samples from a single bucket; Standardization description: water samples sealed and extracted as samples to provide precision estimate ; Standardization frequency: 1 per set of 10 extractions; CRM manufacturer: CRMs not used in 2011 and 2012; Poison name: mercuric chloride; Poison volume: 10 microliters sat'd solution per 5 mL sample; Poison correction: ampoules were pre-weighed with dried poison in them, then full ampoules weighed to get sample mass; Uncertainty: 'average reproducibility 9.1 umol/kg DIC, or 0.45 percent (between replicate buckets); Researcher name: D McCorkle; Researcher institution: Woods Hole Oceanographic Institution.
  • Parameter or Variable: Total alkalinity; Abbreviation: TA; Unit: micromol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: measured both years; Sampling instrument: syringe, 0.45 um filter; Analyzing instrument: Brinkmann automated small-volume alkalinity titrator; Type of titration: Gran ("two-sided" Gran in Tiamo; not standard Gran); Cell type (open or closed): open; Curve fitting method: Brinkmann Tiamo software; Detailed sampling and analyzing information: 20 mL sample collected and poisoned; 1 mL aliqots weighed (microbalance) and titrated in duplicate; Replicate information: replicate buckets, no replicate samples from a single bucket; Standardization description: secondary seawater standards calibrated with SIO CRMs; Standardization frequency: every 5 samples; CRM manufacturer: Dickson lab, SIO; CRM batch number: several used ; Poison name: mercuric chloride; Poison volume: 10 microliters sat'd solution per 20 mL sample; Poison correction: none; TA blank correction: no separate blank correction; all corrections based on analyses of secondary standards; Uncertainty: average reproducibility 6.4 umol/kg alkalinity, or 0.30 percent (between replicate buckets); Researcher name: D McCorkle; Researcher institution: Woods Hole Oceanographic Institution.
  • Parameter or Variable: pH; Abbreviation: pH; pH scale: total; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: measured both years; Sampling instrument: syringe, 0.45 um filter; Analyzing instrument: Ocean Optics spectrophotometer with Z cell; Temperature of pH measurement: measured for each sample; Z cell immersed in experimental water bath; Replicate information: replicate buckets, no replicate samples from a single bucket; Standardization description: no independent pH standardization; At what temperature was pH reported: reported at measurement temperature; Uncertainty: average analytical reproducibility 0.00195 pH units (vs. between-bucket reproducibility of 0.022 units); Researcher name: D McCorkle; Researcher institution: Woods Hole Oceanographic Institution.
  • Parameter or Variable: pCO2 (fCO2) autonomous; Abbreviation: pCO2; Unit: ppmv; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: calculated (from dic and alk in 2011, from pH and alk in 2012); At what temperature was pCO2 reported: 19.51 C; Researcher name: D McCorkle; Researcher institution: Woods Hole Oceanographic Institution.
  • Parameter or Variable: Number of animals alive per container taken at each water change; Abbreviation: Live ; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Graduated cylinder and eppendorf pipette; Analyzing instrument: Olympus inverted microscope.; Duration: Experiment studied; Detailed sampling and analyzing information: During each water change, animals were concentrated to a known volume (usually 500 ml) a subset of this was removed and preserved with buffered formalin. After the experiment samples were analyzed for live and dead. Number of live animals per sample was then multiplied out to calculate the number of live aniamals per bucket.; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: James Widman; Researcher institution: National Marine Fisheries Service .
  • Parameter or Variable: Number of dead animals per container observed at each water change; Abbreviation: Dead; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Graduated cylinder and eppendorf pipette; Analyzing instrument: Olympus inverted microscope.; Duration: Experiment studied; Detailed sampling and analyzing information: During each water change, animals were concentrated to a known volume (usually 500 ml) a subset of this was removed and preserved with buffered formalin. After the experiment samples were analyzed for live and dead. The number of dead animals in the sample was then multiplied out to calculate the number of dead aniamals per bucket.; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: James Widman; Researcher institution: National Marine Fisheries Service .
  • Parameter or Variable: Mean Shell length; Abbreviation: Mean Shell length; Unit: um; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: measured; Sampling instrument: Graduated cylinder and eppendorf pipette; Analyzing instrument: Olympus Inverted Microscope and Image J software; Duration: Experiment studied; Detailed sampling and analyzing information: During each water change, animals were concentrated to a known volume (usually 500 ml) a subset of this was removed and preserved with buffered formalin. After the experiment samples were analyzed for shell length (longest portion parallell to the hinge). Images were taken via an Olympus inverted microscope and analyzed via ImageJ.; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: James Widman; Researcher institution: National Marine Fisheries Service .
  • Parameter or Variable: Mean weight of 50 animals; Abbreviation: Mean weight of 50 animals; Unit: ug; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: measured; Sampling instrument: Graduated cylinder and eppendorf pipette; Analyzing instrument: Mettler MX5 microbalance; Duration: Experiment studied; Detailed sampling and analyzing information: Shell weights were analyzed by rinsing an aliquot of larvae (removed from a known volume of larvae distributed in sea water) in ethanol, then dried at 60C for a minimum of 12 hours. Groups of 50 individuals were picked using a fine paintbrush and weighted on a Mettler MX5 microbalance.; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Kathryn Rose Pietro; Researcher institution: Woods Hole Oceanographic Institution.
  • Parameter or Variable: Temperature; Abbreviation: Temperature; Unit: degrees C; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: measured; Sampling instrument: Onset temperature loggers; Duration: Experiment studied; Detailed sampling and analyzing information: mean temperature in water bath over the experimental duration; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Daniel McCorkle; Researcher institution: Woods Hole Oceanographic Institution.
  • Parameter or Variable: Salinity; Abbreviation: Salinity; Unit: psu; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: measured; Sampling instrument: syringe, not filtered; Analyzing instrument: Autosal salinometer; Duration: Experiment studied; Detailed sampling and analyzing information: samples analyzed in the Woods Hole Oceanographic Institution hydrographic lab; Uncertainty: better than 0.01 psu; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: D McCorkle; Researcher institution: Woods Hole Oceanographic Institution.
  • Parameter or Variable: Aragonite Saturation State; Abbreviation: Aragonite Saturation State; Unit: omega (aragonite) ; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: manipulation condition; Measured or calculated: calculated; Duration: Experiment studied; Detailed sampling and analyzing information: calculated using CO2sys program; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Daniel McCorkle; Researcher institution: Woods Hole Oceanographic Institution.
  • Parameter or Variable: Dissolved Phosphate; Abbreviation: PO4; Unit: micro mol/Kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Analyzing instrument: Quattro, Test range 0-4 umol/L, 10 mm flowcell with LED photometer at 880 nm, 60 samples/hr, standard deviation is 0.01 umol/L, detetion limit is 0.004umol/L; Duration: Experiment studied; Detailed sampling and analyzing information: Samples were placed in acid washed 15 or 50 ml plastic centrifuge tubes and frozen at -20. Samples were thawed prior to analysis.; Method reference: Method NO Q-066-05 Rev. 3 for QuAAtro Applications developed by the Royal Netherlands Institute for Sea Research; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Shannon Meseck; Researcher institution: National Marine Fisheries Service .
  • Parameter or Variable: Dissolved nitrate+nitrite; Abbreviation: NO3+NO2; Unit: micro mol/Kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Response variable; Analyzing instrument: Quattro, Test range 0-250 umol/L, 10 mm flowcell with LED photometer at 520nm, 90 samples/hr, standard deviation is 0.03 umol/L, detetion limit is 0.02 umol/L; Duration: Experiment studied; Detailed sampling and analyzing information: Samples were placed in acid washed 15 or 50 ml plastic centrifuge tubes and frozen at -20. Samples were thawed prior to analysis.; Method reference: Method NO Q-068-05 Rev. 4 for QuAAtro Applications developed by the Royal Netherlands Institute for Sea Research; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Shannon Meseck; Researcher institution: National Marine Fisheries Service .
  • Parameter or Variable: Dissolved nitrite; Abbreviation: NO2; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Analyzing instrument: Quattro, Test range 0-250 umol/L, 10 mm flowcell with LED photometer at 520nm, 90 samples/hr, standard deviation is 0.03 umol/L, detetion limit is 0.02 umol/L; Duration: Experiment studied; Detailed sampling and analyzing information: Samples were placed in acid washed 15 or 50 ml plastic centrifuge tubes and frozen at -20. Samples were thawed prior to analysis.; Method reference: Method NO Q-068-05 Rev. 4 for QuAAtro Applications developed by the Royal Netherlands Institute for Sea Research; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Shannon Meseck; Researcher institution: National Marine Fisheries Service .
  • Parameter or Variable: dissolved silicate; Abbreviation: Si; Unit: micro mol/Kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: measured; Analyzing instrument: Quattro, Test range0-20umol/L, 10 mm flowcell with LED photometer at 820 nm, 60 samples/hr, standard deviation is 0.05 umol/L, detetion limit is 0.01 umol/L; Duration: Experiment studied; Detailed sampling and analyzing information: Samples were placed in acid washed 15 or 50 ml plastic centrifuge tubes and frozen at -20. Samples were thawed prior to analysis.; Method reference: Method NO Q-066-05 Rev. 3 for QuAAtro Applications developed by the Royal Netherlands Institute for Sea Research; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Shannon Meseck; Researcher institution: National Marine Fisheries Service .
  • Parameter or Variable: Ammonia; Abbreviation: NH4; Unit: micro mol/Kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: measured; Analyzing instrument: Quattro, Test range0-20umol/L, 10 mm flowcell with LED photometer at 820 nm, 60 samples/hr, standard deviation is 0.05 umol/L, detetion limit is 0.01 umol/L; Duration: Experiment studied; Detailed sampling and analyzing information: Samples were placed in acid washed 15 or 50 ml plastic centrifuge tubes and frozen at -20. Samples were thawed prior to analysis.; Method reference: Method NO Q-068-05 Rev. 4 for QuAAtro Applications developed by the Royal Netherlands Institute for Sea Research; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Shannon Meseck; Researcher institution: National Marine Fisheries Service .
  • Parameter or Variable: Dissolved nitrate; Abbreviation: NO3 ; Unit: micro mol/Kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: measured; Analyzing instrument: Quattro, Test range 0-250 umol/L, 10 mm flowcell with LED photometer at 520nm, 90 samples/hr, standard deviation is 0.03 umol/L, detetion limit is 0.02 umol/L; Duration: Experiment studied; Detailed sampling and analyzing information: Samples were placed in acid washed 15 or 50 ml plastic centrifuge tubes and frozen at -20. Samples were thawed prior to analysis.; Method reference: Method NO Q-068-05 Rev. 4 for QuAAtro Applications developed by the Royal Netherlands Institute for Sea Research; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Shannon Meseck; Researcher institution: National Marine Fisheries Service .
  • Parameter or Variable: hydrocarbons; Abbreviation: HC; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Analyzing instrument: Iatroscan Model number MK-6s; Duration: Experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned. ; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Jeanette Wells; Researcher institution: Memorial University of Newfoundland.
  • Parameter or Variable: steryl esters/wax esters; Abbreviation: SE/WE; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Analyzing instrument: Iatroscan Model number MK-6s; Duration: Experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned. ; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Jeanette Wells; Researcher institution: Memorial University of Newfoundland.
  • Parameter or Variable: ethyl esters; Abbreviation: EE; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles. ; Analyzing instrument: Iatroscan Model number MK-6s; Duration: Experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned. ; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Jeanette Wells; Researcher institution: Memorial University of Newfoundland.
  • Parameter or Variable: methyl esters; Abbreviation: ME; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles. ; Analyzing instrument: Iatroscan Model number MK-6s; Duration: Experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned. ; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Jeanette Wells; Researcher institution: Memorial University of Newfoundland.
  • Parameter or Variable: ethyl ketones; Abbreviation: EK; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles. ; Analyzing instrument: Iatroscan Model number MK-6s; Duration: Experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned. ; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Jeanette Wells; Researcher institution: Memorial University of Newfoundland.
  • Parameter or Variable: methyl ketones; Abbreviation: MK; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles. ; Analyzing instrument: Iatroscan Model number MK-6s; Duration: Experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned. ; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Jeanette Wells; Researcher institution: Memorial University of Newfoundland.
  • Parameter or Variable: gylcerol ethers; Abbreviation: GE; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles. ; Analyzing instrument: Iatroscan Model number MK-6s; Duration: Experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned. ; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Jeanette Wells; Researcher institution: Memorial University of Newfoundland.
  • Parameter or Variable: Triacylglycerols; Abbreviation: TAG; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles. ; Analyzing instrument: Iatroscan Model number MK-6s; Duration: Experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned. ; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Jeanette Wells; Researcher institution: Memorial University of Newfoundland.
  • Parameter or Variable: free fatty acids; Abbreviation: FFA; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles. ; Analyzing instrument: Iatroscan Model number MK-6s; Duration: Experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned. ; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Jeanette Wells; Researcher institution: Memorial University of Newfoundland.
  • Parameter or Variable: Alcohols; Abbreviation: AL; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles. ; Analyzing instrument: Iatroscan Model number MK-6s; Duration: Experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned. ; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Jeanette Wells; Researcher institution: Memorial University of Newfoundland.
  • Parameter or Variable: Sterols; Abbreviation: ST ; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles. ; Analyzing instrument: Iatroscan Model number MK-6s; Duration: Experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned. ; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Jeanette Wells; Researcher institution: Memorial University of Newfoundland.
  • Parameter or Variable: diacylglycerols; Abbreviation: DAG; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles. ; Analyzing instrument: Iatroscan Model number MK-6s; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned. ; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Jeanette Wells; Researcher institution: Memorial University of Newfoundland.
  • Parameter or Variable: acetone mobile polar lipids; Abbreviation: AMPL; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles. ; Analyzing instrument: Iatroscan Model number MK-6s; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned. ; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Jeanette Wells; Researcher institution: Memorial University of Newfoundland.
  • Parameter or Variable: Phospholipids; Abbreviation: PL; Unit: % of total lipids; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: 0.25 in Inner Diamter X 0.375 in Outer Diamter Silicone Tubing into 1 L amber bottles. ; Analyzing instrument: Iatroscan Model number MK-6s; Duration: experiment studied; Detailed sampling and analyzing information: All samples were collected on combusted (500 C) GF/C filters (1 l minimum filtered) and stored in glass test tubes that were lipid cleaned. ; Method reference: Parrish, CC (1999. Determination of total lipid, lipid classes and fatty acids in equatic samples. Lipids in freshwater ecosytems. MA arts, BC wainman New York, Springer Verlag:4-12; Biological subject: Surfclam; Species ID: Spisula solidissima; Researcher name: Jeanette Wells; Researcher institution: Memorial University of Newfoundland.
Lineage information for: repository
Processing Steps
  • 2015-04-22T00:00:00 - NOAA created the National Centers for Environmental Information (NCEI) by merging NOAA's National Climatic Data Center (NCDC), National Geophysical Data Center (NGDC), and National Oceanographic Data Center (NODC), including the National Coastal Data Development Center (NCDDC), per the Consolidated and Further Continuing Appropriations Act, 2015, Public Law 113-235. NCEI launched publicly on April 22, 2015.
Acquisition Information (collection)
Instrument
  • spectrophotometer
Last Modified: 2018-09-17T20:22:20
For questions about the information on this page, please email: NODC.DataOfficer@noaa.gov