The effects of ocean acidification on hemocyte of crab species in Alaska from laboratory experiment studies from 2011-07-01 to 2013-07-06 (NODC Accession 0123400)

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This archival package contains laboratory experiment data that were collected to examine the effects of ocean acidification on hemocyte of crab species in Alaska. Flow cytometry provides a rapid and reproducible method for analyzing crustacean hemocytes and their functions under experimentally-varied environmental conditions. We used flow cytometry to determine if there was a difference in hematology and selected immune functions, and intracellular pH (pHi), under two different, future ocean acidification scenarios (pH = 7.51, 7.80) compared to current conditions (pH = 8.06) for Chionoecetes bairdi, the Tanner crab. Hemocytes were analyzed after adult Tanner crabs were held for two years under continuous exposure to acidified ocean water. Total counts of hemocytes did not vary among experimental control and treatments; however, there was a significantly greater number of dead, circulating hemocytes in crabs held at the lowest pH treatment. Phagocytosis of fluorescent microbeads by hemocytes was greatest at the lowest pH treatment. These results suggest that hemocytes were dying, likely by apoptosis, at a rate faster than upregulated phagocytosis was able to remove moribund cells from circulation at the lowest pH. There was no significant difference in pHi within hyalinocytes among pH levels, with apparent regulation to a mean pHi of 7.24, significantly lower than the external environment. In contrast, there was a significant difference between treatments in pHi of the semi-granular+granular cells. Control crabs had the highest mean semi-granular+granular cell intracellular pHi of 8.27 plus/minus 0.08 (S.E., standard error) compared to the lowest pH treatment, which had a pHi of 7.73 plus/minus 0.10 (S.E.). These findings suggest that future, predicted levels of ocean acidification may affect the defense cells of Tanner crabs, possibly making them more susceptible to other stressors in the environment.
  • Cite as: Meseck, Shannon L.; Alix, Jennifer H.; Swiney, Katherine M.; Long, Christopher W.; Wikfors, Gary H.; Foy, Robert J. (2015). The effects of ocean acidification on hemocyte of crab species in Alaska from laboratory experiment studies from 2011-07-01 to 2013-07-06 (NODC Accession 0123400). Version 1.1. National Oceanographic Data Center, NOAA. Dataset. doi:10.7289/V59884ZW [access date]
gov.noaa.nodc:0123400
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Distributor DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
301-713-3277
NCEI.Info@noaa.gov
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DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
301-713-3277
NCEI.Info@noaa.gov
Time Period 2011-07-01 to 2013-07-06
Spatial Bounding Box Coordinates
N: 57.7208
S: 57.7208
E: -152.2917
W: -152.2917
Spatial Coverage Map
General Documentation
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Publication Dates
  • publication: 2015-01-13
Edition 1.1
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Data Update Frequency As needed
Supplemental Information
PRINCIPAL INVESTIGATORS: Foy, Robert; Long, Chris; and Swiney, Katherine.
FUNDING AGENCY: NOAA's Ocean Acidification Program (OAP).
PROJECT TITLE: Effects of ocean acidification on federally managed crab species in Alaska.
PROJECT ID: OAPFY13.03.AFSC.001.
Purpose To determine if hemocytes were effect during ocean acidification
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Meseck, Shannon L.; Alix, Jennifer H.; Swiney, Katherine M.; Long, Christopher W.; Wikfors, Gary H.; Foy, Robert J. (2015). The effects of ocean acidification on hemocyte of crab species in Alaska from laboratory experiment studies from 2011-07-01 to 2013-07-06 (NODC Accession 0123400). Version 1.1. National Oceanographic Data Center, NOAA. Dataset. doi:10.7289/V59884ZW [access date]
Cited Authors
  • Meseck, Shannon L.
  • Alix, Jennifer H.
  • Swiney, Katherine M.
  • Long, Christopher W.
  • Wikfors, Gary H.
  • Foy, Robert J.
Principal Investigators
Contributors
Resource Providers
Publishers
Acknowledgments
  • Funding Information: NOAA's Ocean Acidification Program (Effects of ocean acidification on federally managed crab species in Alaska, OAPFY13.03.AFSC.001)
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
Global Change Master Directory (GCMD) Science and Services Keywords
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH
Provider Variable Names
  • % Dead Cell SGC+GC
  • % Dead HC
  • % Phagocytosis
  • Average pH
  • Calculated HC pHi
  • Calculated SGC+GC pHi
  • Dead Cell HC
  • Dead Cell SGC+GC
  • Hemolymph pHe
  • Raw data SNARF-5F HC FL2-H to calculate internal cell pH
  • Raw data SNARF-5F SGC+GC FL2-H to calculate internal cell pH
  • Raw data SNARF-5F SNARF-5F HC FL3-H used to calculate internal pH
  • Raw data SNARF-5F SNARF-5F SGC+GC FL3-H used to calculate internal pH
  • Total Cell HC
  • Total Cell SGC+GC
Data Center keywords Global Change Master Directory (GCMD) Data Center Keywords
  • DOC/NOAA/NESDIS/NODC > National Oceanographic Data Center, NESDIS, NOAA, U.S. Department of Commerce
  • DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
  • DOC/NOAA/NMFS/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce
  • DOC/NOAA/NMFS/NEFSC > Northeast Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce
NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS
Instrument keywords NODC INSTRUMENT TYPES THESAURUS Global Change Master Directory (GCMD) Instrument Keywords
  • FLOW CYTOMETRY
Place keywords NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords
  • OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN
  • OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN > GULF OF ALASKA
Provider Geographic Names
  • Gulf of Alaska
  • North Pacific Ocean
Project keywords NODC PROJECT NAMES THESAURUS Ocean Acidification Search Keywords
  • Ocean Acidification Data Stewardship (OADS) Project
  • Ocean Acidification Program (OAP)
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Meseck, Shannon L.; Alix, Jennifer H.; Swiney, Katherine M.; Long, Christopher W.; Wikfors, Gary H.; Foy, Robert J. (2015). The effects of ocean acidification on hemocyte of crab species in Alaska from laboratory experiment studies from 2011-07-01 to 2013-07-06 (NODC Accession 0123400). Version 1.1. National Oceanographic Data Center, NOAA. Dataset. doi:10.7289/V59884ZW [access date]
Access Constraints
  • NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
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Processing Steps
  • 2015-01-13T14:24:07 - NCEI Accession 0123400 v1.1 was published.
Output Datasets
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Processing Steps
  • Parameter or Variable: Average pH that Tanner crab was held at for 602 days before their hemolymph was removed and anlayzed for immune response.; Abbreviation: Average pH; Controlled vocabulary name: None; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: calculated average; Analyzing instrument: a Durafet II or III pH probe ; Detailed sampling and analyzing information: This is the average pH that each crab was exposed to for 602 days. The original pH, alkalinity, DIC values can be found in the data set by Lead PI Robert Foy entitled Effects of Ocean Acidification on Federally Managed Crab Species in Alaska ; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Raw data with the probe SNARF-5F used to stain the Semi-granular+ granular cell and give FL2-H flourenced that is used to calculate internal cell pH; Abbreviation: Raw data SNARF-5F SGC+GC FL2-H to calculate internal cell pH; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab. ; Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States). ; Detailed sampling and analyzing information: The mean FL-2 H(580 nm) wavelenght was determined using the software for the semi-granular + ganular cells. The scale for the x and y axis was in the log. Detailed information about how the data is collected and analyzed can be found at the method referenced below.; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water ; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Raw data with the probe SNARF-5F used to stain the Semi-granular+ granular cell and give FL3-H flourenced that is used to calculate internal cell pH; Abbreviation: Raw data SNARF-5F SNARF-5F SGC+GC FL3-H used to calculate internal pH; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab. ; Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States). ; Detailed sampling and analyzing information: The mean FL-3 H(630 nm) wavelenght was determined using the software for the semi-granular + ganular cells. The scale for the x and y axis was in the log. Detailed information about how the data is collected and analyzed can be found at the method referenced below.; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water ; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Raw data with the probe SNARF-5F used to stain the halinocyte cell and give FL2-H flourenced that is used to calculate internal cell pH; Abbreviation: Raw data SNARF-5F HC FL2-H to calculate internal cell pH; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab. ; Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States). ; Detailed sampling and analyzing information: The mean FL-2 H(580 nm) wavelenght was determined using the software for the halinocytes cells. The scale for the x and y axis was in the log. Detailed information about how the data is collected and analyzed can be found at the method referenced below.; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water ; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Raw data with the probe SNARF-5F used to stain the halinocyte cell and give FL3-H flourenced that is used to calculate internal cell pH; Abbreviation: Raw data SNARF-5F SNARF-5F HC FL3-H used to calculate internal pH; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab. ; Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States). ; Detailed sampling and analyzing information: The mean FL-3 H(630 nm) wavelenght was determined using the software for the semi-granular + ganular cells. The scale for the x and y axis was in the log. Detailed information about how the data is collected and analyzed can be found at the method refer; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water ; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Calcualted semi-granular and granular cell internal pH ; Abbreviation: Calculated SGC+GC pHi; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Calculated; Analyzing instrument: The ratio of FL3/FL2 from a calibration curve was used to generate the internal pH of the cells. The calibration curve can be found in the reference below. Detailed information about how the data is collected and analyzed can be found at the method referenced below.; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water ; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Calcualted halinocyte cell internal pH ; Abbreviation: Calculated HC pHi; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Calculated; Analyzing instrument: The ratio of FL3/FL2 from a calibration curve was used to generate the internal pH of the cells. The calibration curve can be found in the reference below. Detailed information about how the data is collected and analyzed can be found at the method referenced below. ; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water ; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: External hemolymph pH; Abbreviation: Hemolymph pHe; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab. ; Analyzing instrument: Corning pH-30 electrode; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water ; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Total number of cells in the hemolymph that are semi-granular and granular hemocytes; Abbreviation: Total Cell SGC+GC; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab. ; Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States). ; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water ; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Total number of cells in the hemolymph that are halinocyte cells; Abbreviation: Total Cell HC; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab. ; Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States). ; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water ; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Total number of dead cells in the hemolymph that are semi-granular and granular hemocytes; Abbreviation: Dead Cell SGC+GC; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab. ; Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States). ; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water ; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Total number of dead halinocyte cells in the hemolymph; Abbreviation: Dead Cell HC; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab. ; Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States). ; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water ; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Percentage of dead cells for semi-granular and granular hemocytes in the hemolymph; Abbreviation: % Dead Cell SGC+GC; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Calculated; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water ; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Percentage of dead halinocyte cells in the hemolymph; Abbreviation: % Dead HC; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Calculated; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water ; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Percentage of cellsthat have Phagocytosis properties; Abbreviation: % Phagocytosis ; Observation type: experiment studied; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab. ; Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States). ; Detailed sampling and analyzing information: All hemocytes that contained 3 beads or more are considered to be highly phagocytic; Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water ; Biological subject: Chionoecetes baird, Tanner Crab; Researcher name: Shannon Meseck; Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
Lineage information for: repository
Processing Steps
  • 2015-04-22T00:00:00 - NOAA created the National Centers for Environmental Information (NCEI) by merging NOAA's National Climatic Data Center (NCDC), National Geophysical Data Center (NGDC), and National Oceanographic Data Center (NODC), including the National Coastal Data Development Center (NCDDC), per the Consolidated and Further Continuing Appropriations Act, 2015, Public Law 113-235. NCEI launched publicly on April 22, 2015.
Acquisition Information (collection)
Instrument
  • Flow Cytometer
Last Modified: 2018-04-27T05:51:18
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