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2024-02-08T23:43:24Z
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ISO 19115-2:2009(E)
The effects of ocean acidification on hemocyte of crab species in Alaska from laboratory experiment studies from 2011-07-01 to 2013-07-06 (NCEI Accession 0123400)
2015-01-13
publication
2021-10-07
revision
International DOI Foundation (IDF)
10.7289/v59884zw
NOAA National Centers for Environmental Information
gov.noaa.nodc:0123400
NCEI Archive Management System
0123400
NOAA National Centers for Environmental Information
ncei.info@noaa.gov
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publisher
Shannon L. Meseck
US DOC; NOAA; NMFS; Northeast Fisheries Science Center; Milford Laboratory (NMFS/NEFSC)
Shannon.Meseck@noaa.gov
http://nefsc.noaa.gov/nefsc/Milford/
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US DOC; NOAA; NMFS; Northeast Fisheries Science Center; Milford Laboratory website
Institution web page
information
resourceProvider
US DOC; NOAA; NMFS; Northeast Fisheries Science Center; Milford Laboratory (NMFS/NEFSC)
http://nefsc.noaa.gov/nefsc/Milford/
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US DOC; NOAA; NMFS; Northeast Fisheries Science Center; Milford Laboratory website
Institution web page
information
resourceProvider
Robert J. Foy
US DOC; NOAA; NMFS; Alaska Fisheries Science Center (NMFS/AFSC)
Robert.Foy@noaa.gov
http://www.afsc.noaa.gov
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NOAA Alaska Fisheries Science Center website
Institution web page
information
principalInvestigator
W. Christopher Long
US DOC; NOAA; NMFS; Alaska Fisheries Science Center (NMFS/AFSC)
chris.long@noaa.gov
http://www.afsc.noaa.gov
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NOAA Alaska Fisheries Science Center website
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information
principalInvestigator
Katherine M. Swiney
US DOC; NOAA; NMFS; Alaska Fisheries Science Center (NMFS/AFSC)
Katherine.Swiney@noaa.gov
principalInvestigator
Meseck, Shannon L.
US DOC; NOAA; NMFS; Northeast Fisheries Science Center; Milford Laboratory
Shannon.Meseck@noaa.gov
http://nefsc.noaa.gov/nefsc/Milford/
HTTP
US DOC; NOAA; NMFS; Northeast Fisheries Science Center; Milford Laboratory website
Institution web page
information
author
Alix, Jennifer H.
author
Swiney, Katherine M.
NOAA Alaska Fisheries Science Center (NMFS/AFSC)
author
Long, W. Christopher
NOAA Alaska Fisheries Science Center (NMFS/AFSC)
chris.long@noaa.gov
http://www.afsc.noaa.gov
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NOAA Alaska Fisheries Science Center website
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information
author
Wikfors, Gary H.
author
Foy, Robert J.
NOAA Alaska Fisheries Science Center (NMFS/AFSC)
Robert.Foy@noaa.gov
http://www.afsc.noaa.gov
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NOAA Alaska Fisheries Science Center website
Institution web page
information
author
US DOC; NOAA; NMFS; Alaska Fisheries Science Center (NMFS/AFSC)
http://www.afsc.noaa.gov
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NOAA Alaska Fisheries Science Center website
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contributor
tableDigital
Flow cytometry provides a rapid and reproducible method for analyzing crustacean hemocytes and their functions under experimentally-varied environmental conditions. We used flow cytometry to determine if there was a difference in hematology and selected immune functions, and intracellular pH (pHi), under two different, future ocean acidification scenarios (pH = 7.51, 7.80) compared to current conditions (pH = 8.06) for Chionoecetes bairdi, the Tanner crab. Hemocytes were analyzed after adult Tanner crabs were held for two years under continuous exposure to acidified ocean water. Total counts of hemocytes did not vary among experimental control and treatments; however, there was a significantly greater number of dead, circulating hemocytes in crabs held at the lowest pH treatment. Phagocytosis of fluorescent microbeads by hemocytes was greatest at the lowest pH treatment. These results suggest that hemocytes were dying, likely by apoptosis, at a rate faster than upregulated phagocytosis was able to remove moribund cells from circulation at the lowest pH. There was no significant difference in pHi within hyalinocytes among pH levels, with apparent regulation to a mean pHi of 7.24, significantly lower than the external environment. In contrast, there was a significant difference between treatments in pHi of the semi-granular+granular cells. These findings suggest that future, predicted levels of ocean acidification may affect the defense cells of Tanner crabs, possibly making them more susceptible to other stressors in the environment.
To determine if hemocytes were effect during ocean acidification
Funding Information: NOAA Ocean Acidification Program (Effects of ocean acidification on federally managed crab species in Alaska, OAPFY13.03.AFSC.001)
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0123400
NCEI ACCESSION NUMBER
2021-09-27
publication
biological data
CELL COUNTS
CRUSTACEANS
MICROBIOLOGY
pH
SHELLFISH
theme
NODC DATA TYPES THESAURUS
Flow Cytometer
instrument
NODC INSTRUMENT TYPES THESAURUS
biological
laboratory analyses
theme
NODC OBSERVATION TYPES THESAURUS
US DOC; NOAA; NMFS; Alaska Fisheries Science Center
dataCentre
NODC COLLECTING INSTITUTION NAMES THESAURUS
US DOC; NOAA; NMFS; Northeast Fisheries Science Center; Milford Laboratory
dataCentre
NODC SUBMITTING INSTITUTION NAMES THESAURUS
NODC Ocean Acidification Scientific Data Stewardship (OADS)
Ocean Carbon and Acidification Data System (OCADS)
US DOC; NOAA; Office of Oceanic and Atmospheric Research; Ocean Acidification Program (OAP)
project
NODC PROJECT NAMES THESAURUS
Gulf of Alaska
North Pacific Ocean
place
NODC SEA AREA NAMES THESAURUS
oceanography
theme
WMO_CategoryCode
2012-09-15
publication
DOC/NOAA/NESDIS/NODC > National Oceanographic Data Center, NESDIS, NOAA, U.S. Department of Commerce
DOC/NOAA/NMFS/AFSC > Alaska Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce
DOC/NOAA/NMFS/NEFSC > Northeast Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce
dataCentre
Global Change Master Directory (GCMD) Data Center Keywords
2023
revision
17
Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)
Greenbelt
MD
https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
HTTPS
GCMD Keyword Forum Page
Global Change Master Directory (GCMD). 2023. GCMD Keywords, Version 17. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords
information
custodian
Ocean Acidification Program (OAP)
Ocean Carbon and Acidification Data System (OCADS) Project
project
Ocean Acidification Search Keywords
EARTH SCIENCE > BIOLOGICAL CLASSIFICATION
EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > ARTHROPODS > CRUSTACEANS
EARTH SCIENCE > BIOLOGICAL CLASSIFICATION > ANIMALS/INVERTEBRATES > MOLLUSKS
EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH
theme
Global Change Master Directory (GCMD) Science Keywords
2023
revision
17
Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)
Greenbelt
MD
https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
HTTPS
GCMD Keyword Forum Page
Global Change Master Directory (GCMD). 2023. GCMD Keywords, Version 17. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords
information
custodian
Laboratory experiment
theme
OCADS Study Type
external hemolymph pH
hemocytes
internal cell pH
intracellular pH
theme
Provider Keywords
% Dead Cell SGC+GC
% Dead HC
% Phagocytosis
Average pH
Calculated HC pHi
Calculated SGC+GC pHi
Dead Cell HC
Dead Cell SGC+GC
Hemolymph pHe
Raw data SNARF-5F HC FL2-H to calculate internal cell pH
Raw data SNARF-5F SGC+GC FL2-H to calculate internal cell pH
Raw data SNARF-5F SNARF-5F HC FL3-H used to calculate internal pH
Raw data SNARF-5F SNARF-5F SGC+GC FL3-H used to calculate internal pH
Total Cell HC
Total Cell SGC+GC
theme
Provider Variable Abbreviations
FLOW CYTOMETRY
instrument
Global Change Master Directory (GCMD) Instrument Keywords
2023
revision
17
Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)
Greenbelt
MD
https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
HTTPS
GCMD Keyword Forum Page
Global Change Master Directory (GCMD). 2023. GCMD Keywords, Version 17. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords
information
custodian
OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN
OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN > GULF OF ALASKA
place
Global Change Master Directory (GCMD) Location Keywords
2023
revision
17
Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA)
Greenbelt
MD
https://forum.earthdata.nasa.gov/app.php/tag/GCMD%2BKeywords
HTTPS
GCMD Keyword Forum Page
Global Change Master Directory (GCMD). 2023. GCMD Keywords, Version 17. Greenbelt, MD: Earth Science Data and Information System, Earth Science Projects Division, Goddard Space Flight Center (GSFC), National Aeronautics and Space Administration (NASA). URL (GCMD Keyword Forum Page): https://forum.earthdata.nasa.gov/app.php/tag/GCMD+Keywords
information
custodian
Gulf of Alaska
North Pacific Ocean
place
Provider Geographic Names
accessLevel: Public
otherRestrictions
Cite as: Meseck, Shannon L.; Alix, Jennifer H.; Swiney, Katherine M.; Long, W. Christopher; Wikfors, Gary H.; Foy, Robert J. (2015). The effects of ocean acidification on hemocyte of crab species in Alaska from laboratory experiment studies from 2011-07-01 to 2013-07-06 (NCEI Accession 0123400). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://doi.org/10.7289/v59884zw. Accessed [date].
Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
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Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Meseck SL, Alix JH, Swiney KM, Long WC, Wikfors GH, Foy RJ (2016) Ocean Acidification Affects Hemocyte Physiology in the Tanner Crab (Chionoecetes bairdi). PLoS ONE 11(2): e0148477. https://doi.org/10.1371/journal.pone.0148477
2016-02-09
publication
International DOI Foundation (IDF)
https://doi.org/10.1371/journal.pone.0148477
https://doi.org/10.1371/journal.pone.0148477
HTTPS
https://doi.org/10.1371/journal.pone.0148477
journal article
information
Meseck, Shannon L.
author
Alix, Jennifer H.
author
Swiney, Katherine M.
author
Long, W. Christopher
author
Wikfors, Gary H.
author
Foy, Robert J.
author
Melzner, Frank
editor
Public Library of Science (PLoS)
publisher
documentDigital
PLoS ONE
11(2)
ISSN 1932-6203, p. e0148477
crossReference
project
NOAA National Centers for Environmental Information (2022). Ocean Carbon and Acidification Data System (OCADS). NOAA National Centers for Environmental Information. https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system
2022-04
publication
https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system
HTTPS
https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system
OCADS website
information
NOAA National Centers for Environmental Information
author
US DOC; NOAA; NESDIS; National Centers for Environmental Information (NCEI)
publisher
documentDigital
[accessed 2024-02-08]
crossReference
program
eng; USA
utf8
environment
oceans
biota
-152.2917
-152.2917
57.7208
57.7208
2011-07-01
2013-07-06
PRINCIPAL INVESTIGATORS: Foy, Robert; Long, Chris; and Swiney, Katherine.
FUNDING AGENCY: NOAA's Ocean Acidification Program (OAP).
PROJECT TITLE: Effects of ocean acidification on federally managed crab species in Alaska.
PROJECT ID: OAPFY13.03.AFSC.001.
In this accession, NCEI has archived multiple versions of these data. The latest (and best) version of these data has the largest version number.
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Originator data format
0.192
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dataset
NCEI Accession 0123400 v1.1 was published.
2015-01-13T14:24:07Z
NCEI Accession 0123400 v1.1
publication
https://www.ncei.noaa.gov/archive/accession/0123400/1.1
HTTPS
NCEI Accession 0123400 v1.1
published 2015-01-13T14:24:07Z
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NCEI Accession 0123400 was revised and v2.2 was published.
Updates were received for this dataset. These updates were copied into the data/0-data/ directory of this accession. These updates may provide additional files or replace obsolete files. This version contains the most complete and up-to-date representation of this archival information package. All of the files received prior to this update are available in the preceding version of this accession.
2021-10-07T21:33:12Z
NCEI Accession 0123400 v2.2
publication
https://www.ncei.noaa.gov/archive/accession/0123400/2.2
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NCEI Accession 0123400 v2.2
published 2021-10-07T21:33:12Z
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dataset
Parameter or Variable: Average pH that Tanner crab was held at for 602 days before their hemolymph was removed and anlayzed for immune response.;
Abbreviation: Average pH;
Controlled vocabulary name: None;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: Manipulation condition;
Measured or calculated: calculated average;
Analyzing instrument: a Durafet II or III pH probe;
Detailed sampling and analyzing information: This is the average pH that each crab was exposed to for 602 days. The original pH, alkalinity, DIC values can be found in the data set by Lead PI Robert Foy entitled Effects of Ocean Acidification on Federally Managed Crab Species in Alaska;
Biological subject: Chionoecetes baird, Tanner Crab;
Researcher name: Shannon Meseck;
Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
Parameter or Variable: Raw data with the probe SNARF-5F used to stain the Semi-granular+ granular cell and give FL2-H fluorenced that is used to calculate internal cell pH;
Abbreviation: Raw data SNARF-5F SGC+GC FL2-H to calculate internal cell pH;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: Response variable;
Measured or calculated: Measured;
Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.;
Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States).;
Detailed sampling and analyzing information: The mean FL-2 H(580 nm) wavelenght was determined using the software for the semi-granular + ganular cells. The scale for the x and y axis was in the log. Detailed information about how the data is collected and analyzed can be found at the method referenced below.;
Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water;
Biological subject: Chionoecetes baird, Tanner Crab;
Researcher name: Shannon Meseck;
Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
Parameter or Variable: Raw data with the probe SNARF-5F used to stain the Semi-granular+ granular cell and give FL3-H fluorenced that is used to calculate internal cell pH;
Abbreviation: Raw data SNARF-5F SNARF-5F SGC+GC FL3-H used to calculate internal pH;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: Response variable;
Measured or calculated: Measured;
Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.;
Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States).;
Detailed sampling and analyzing information: The mean FL-3 H(630 nm) wavelenght was determined using the software for the semi-granular + ganular cells. The scale for the x and y axis was in the log. Detailed information about how the data is collected and analyzed can be found at the method referenced below.;
Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water;
Biological subject: Chionoecetes baird, Tanner Crab;
Researcher name: Shannon Meseck;
Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
Parameter or Variable: Raw data with the probe SNARF-5F used to stain the halinocyte cell and give FL2-H fluorenced that is used to calculate internal cell pH;
Abbreviation: Raw data SNARF-5F HC FL2-H to calculate internal cell pH;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: Response variable;
Measured or calculated: Measured;
Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.;
Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States).;
Detailed sampling and analyzing information: The mean FL-2 H(580 nm) wavelenght was determined using the software for the halinocytes cells. The scale for the x and y axis was in the log. Detailed information about how the data is collected and analyzed can be found at the method referenced below.;
Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water;
Biological subject: Chionoecetes baird, Tanner Crab;
Researcher name: Shannon Meseck;
Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
Parameter or Variable: Raw data with the probe SNARF-5F used to stain the halinocyte cell and give FL3-H fluorenced that is used to calculate internal cell pH;
Abbreviation: Raw data SNARF-5F SNARF-5F HC FL3-H used to calculate internal pH;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: Response variable;
Measured or calculated: Measured;
Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.;
Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States).;
Detailed sampling and analyzing information: The mean FL-3 H(630 nm) wavelenght was determined using the software for the semi-granular + ganular cells. The scale for the x and y axis was in the log. Detailed information about how the data is collected and analyzed can be found at the method refer;
Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water;
Biological subject: Chionoecetes baird, Tanner Crab;
Researcher name: Shannon Meseck;
Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
Parameter or Variable: Calcualted semi-granular and granular cell internal pH;
Abbreviation: Calculated SGC+GC pHi;
In-situ / Manipulation / Response variable: Response variable;
Measured or calculated: Calculated;
Analyzing instrument: The ratio of FL3/FL2 from a calibration curve was used to generate the internal pH of the cells. The calibration curve can be found in the reference below. Detailed information about how the data is collected and analyzed can be found at the method referenced below.;
Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water;
Biological subject: Chionoecetes baird, Tanner Crab;
Researcher name: Shannon Meseck;
Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
Parameter or Variable: Calcualted halinocyte cell internal pH;
Abbreviation: Calculated HC pHi;
In-situ / Manipulation / Response variable: Response variable;
Measured or calculated: Calculated;
Analyzing instrument: The ratio of FL3/FL2 from a calibration curve was used to generate the internal pH of the cells. The calibration curve can be found in the reference below. Detailed information about how the data is collected and analyzed can be found at the method referenced below.;
Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water;
Biological subject: Chionoecetes baird, Tanner Crab;
Researcher name: Shannon Meseck;
Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
Parameter or Variable: External hemolymph pH;
Abbreviation: Hemolymph pHe;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: Response variable;
Measured or calculated: Measured;
Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.;
Analyzing instrument: Corning pH-30 electrode;
Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water;
Biological subject: Chionoecetes baird, Tanner Crab;
Researcher name: Shannon Meseck;
Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
Parameter or Variable: Total number of cells in the hemolymph that are semi-granular and granular hemocytes;
Abbreviation: Total Cell SGC+GC;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: Response variable;
Measured or calculated: Measured;
Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.;
Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States).;
Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water;
Biological subject: Chionoecetes baird, Tanner Crab;
Researcher name: Shannon Meseck;
Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
Parameter or Variable: Total number of cells in the hemolymph that are halinocyte cells;
Abbreviation: Total Cell HC;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: Response variable;
Measured or calculated: Measured;
Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.;
Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States).;
Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water;
Biological subject: Chionoecetes baird, Tanner Crab;
Researcher name: Shannon Meseck;
Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
Parameter or Variable: Total number of dead cells in the hemolymph that are semi-granular and granular hemocytes;
Abbreviation: Dead Cell SGC+GC;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: Response variable;
Measured or calculated: Measured;
Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.;
Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States).;
Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water;
Biological subject: Chionoecetes baird, Tanner Crab;
Researcher name: Shannon Meseck;
Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
Parameter or Variable: Total number of dead halinocyte cells in the hemolymph;
Abbreviation: Dead Cell HC;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: Response variable;
Measured or calculated: Measured;
Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.;
Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States).;
Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water;
Biological subject: Chionoecetes baird, Tanner Crab;
Researcher name: Shannon Meseck;
Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
Parameter or Variable: Percentage of dead cells for semi-granular and granular hemocytes in the hemolymph;
Abbreviation: % Dead Cell SGC+GC;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: Response variable;
Measured or calculated: Calculated;
Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water;
Biological subject: Chionoecetes baird, Tanner Crab;
Researcher name: Shannon Meseck;
Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
Parameter or Variable: Percentage of dead halinocyte cells in the hemolymph;
Abbreviation: % Dead HC;
Observation type: Laboratory experiment;
In-situ / Manipulation / Response variable: Response variable;
Measured or calculated: Calculated;
Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water;
Biological subject: Chionoecetes baird, Tanner Crab;
Researcher name: Shannon Meseck;
Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
Parameter or Variable: Percentage of cellsthat have Phagocytosis properties;
Abbreviation: % Phagocytosis;
Observation type: experiment studied;
In-situ / Manipulation / Response variable: Response variable;
Measured or calculated: Measured;
Sampling instrument: A 2-ml 23-gauge, sterile needle was used to collect hemolymph from each crab.;
Analyzing instrument: Accuri C6 flow cytometer (Becton Dickinson, United States) for 30 seconds at 66 ul min-1. Side-scatter, forward-scatter, and fluorescence intensities of individual cells were collected and analyzed using BD Accuri C6 Software (Becton-Dickinson, United States).;
Detailed sampling and analyzing information: All hemocytes that contained 3 beads or more are considered to be highly phagocytic;
Method reference: Shannon L. Meseck, Jennifer H. Alix, Katherine M. Swiney, W. Christopher Long, Gary H. Wikfors, Robert J. Foy (submitted PLOS One, accepted unsure of publication date). Flow cytometric characterization of hemocytes from the Tanner crab (Chionoecetes bairdi) subjected to acidified ocean water;
Biological subject: Chionoecetes baird, Tanner Crab;
Researcher name: Shannon Meseck;
Researcher institution: Northeast Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration .
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Flow Cytometer
Flow Cytometer
A method to enumerate bacterial and phytoplankton cells in seawater and estimate their spherical diameter
Flow cytometers are laboratory based instruments used to measure cells and particles from aqueous samples. The instrument was initially developed for analysis of Human blood cells for medical purposes, but this technology was adopted by the ocean science community by several investigators, principally Penny Chischolm out of the Massachusetts Institute of Technology, in late 20th century. Since then, the technique has been broadly applied, and it is a unique measurement of microbial populations in the ocean because it provides one at a time measures of cells, to extrapolate features such as fluorescence and cell size. Flow cytometers have been adapted for a number of oceanographic applications such as ship board measurement, and more precise measurement of planktonic fractions. Additionally, the flow cytometer has been adapted for in-situ deployment (see the corresponding nodc instrument code).