Effects of ocean acidification on shell condition and survival of Puget Sound pteropods from laboratory experiment studies from 2012-05-10 to 2012-07-12 (NODC Accession 0125008)

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This archival package contains laboratory experiment data that were collected to examine the effects of ocean acidification on shell condition and survival of Puget Sound pteropods. We tested whether the thecosome pteropod Limacina helicina from Puget Sound, an urbanized estuary in the northwest continental US, experiences shell dissolution and altered mortality rates when exposed to the high CO2, low aragonite saturation state (Ωa) conditions that occur in Puget Sound and the northeast Pacific Ocean. Five, week-long experiments were conducted in which we incubated pteropods collected from Puget Sound in four carbon chemistry conditions: current summer surface (~460-500 μatm CO2, Ωa ≈ 1.59), current deep water or surface conditions during upwelling (~760 and ~1600-1700 μatm CO2, , Ωa ≈ 1.17 and 0.56), and future deep water or surface conditions during upwelling (~2800-3400 μatm CO2, Ωa ≈ 0.28). We measured shell condition using a scoring regime of five shell characteristics that capture different aspects of shell dissolution. We characterized carbon chemistry conditions in statistical analyses with Ωa, and conducted analyses considering Ωa both as a continuous dataset and as discrete treatments. Shell dissolution increased linearly as aragonite saturation state decreased. Discrete treatment comparisons indicate that shell dissolution was greater in undersaturated treatments compared to oversaturated treatments. Survival increased linearly with aragonite saturation state, though discrete treatment comparisons indicated that survival was similar in all but the lowest saturation state treatment. These results indicate that, under starvation conditions, pteropod survival may not be greatly affected by current and expected near-future aragonite saturation state in the NE Pacific, but shell dissolution may. Given that subsurface waters in Puget Sound’s main basin are undersaturated with respect to aragonite in the winter and can be undersaturated in the summer, the condition and persistence of the species in this estuary warrants further study.
  • Cite as: Busch, D. Shallin; Maher, Michael; Thibodeau, Patricia S.; McElhany, Paul (2015). Effects of ocean acidification on shell condition and survival of Puget Sound pteropods from laboratory experiment studies from 2012-05-10 to 2012-07-12 (NODC Accession 0125008). Version 1.1. National Oceanographic Data Center, NOAA. Dataset. doi:10.7289/V5S75D83 [access date]
gov.noaa.nodc:0125008
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Distributor DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
301-713-3277
NCEI.Info@noaa.gov
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DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
301-713-3277
NCEI.Info@noaa.gov
Time Period 2012-05-10 to 2012-07-12
Spatial Bounding Box Coordinates
N: 48.1
S: 48.1
E: -122.4
W: -122.4
Spatial Coverage Map
General Documentation
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Publication Dates
  • publication: 2015-01-20
Edition 1.1
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Data Update Frequency As needed
Supplemental Information
PRINCIPAL INVESTIGATORS: D. Shallin Busch; Paul McElhany.
FUNDING AGENCY: NOAA's Ocean Acidification Program (OAP).
PROJECT TITLE: Single species response experiments.
PROJECT ID: OAPFY13.03.NWFSC.002
Purpose To determine whether PCO2 levels influence mortality and shell condition of the pteropod Limacina limacina
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Busch, D. Shallin; Maher, Michael; Thibodeau, Patricia S.; McElhany, Paul (2015). Effects of ocean acidification on shell condition and survival of Puget Sound pteropods from laboratory experiment studies from 2012-05-10 to 2012-07-12 (NODC Accession 0125008). Version 1.1. National Oceanographic Data Center, NOAA. Dataset. doi:10.7289/V5S75D83 [access date]
Cited Authors
  • Busch, D. Shallin
  • Maher, Michael
  • Thibodeau, Patricia S.
  • McElhany, Paul
Principal Investigators
Contributors
Resource Providers
Publishers
Acknowledgments
  • Funding Information: NOAA's Ocean Acidification Program (Single species response experiments, OAPFY13.03.NWFSC.002)
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
Global Change Master Directory (GCMD) Science and Services Keywords
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > ALKALINITY
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > INORGANIC CARBON
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH
  • EARTH SCIENCE > OCEANS > SALINITY/DENSITY > SALINITY
Provider Variable Names
  • Arag
  • Cat1
  • Cat2
  • Cat3
  • Cat4
  • Cat5
  • DIC
  • Dead
  • Durafet
  • Live
  • Ratio
  • Salinity
  • Spec
  • Sum
  • TA
  • pCO2
  • pH
Data Center keywords Global Change Master Directory (GCMD) Data Center Keywords
  • DOC/NOAA/NESDIS/NODC > National Oceanographic Data Center, NESDIS, NOAA, U.S. Department of Commerce
  • DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
  • DOC/NOAA/NMFS/NWFSC > Northwest Fisheries Science Center, National Marine Fisheries Service, NOAA, U.S. Department of Commerce
NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS
Instrument keywords NODC INSTRUMENT TYPES THESAURUS Global Change Master Directory (GCMD) Instrument Keywords
  • OPTSPEC > Optical Spectrometer
Place keywords NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords
  • OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN
Provider Geographic Names
  • Port Susan, Puget Sound, Washington
  • West coast of the U.S.
Project keywords NODC PROJECT NAMES THESAURUS Ocean Acidification Search Keywords
  • Ocean Acidification Data Stewardship (OADS) Project
  • Ocean Acidification Program (OAP)
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Busch, D. Shallin; Maher, Michael; Thibodeau, Patricia S.; McElhany, Paul (2015). Effects of ocean acidification on shell condition and survival of Puget Sound pteropods from laboratory experiment studies from 2012-05-10 to 2012-07-12 (NODC Accession 0125008). Version 1.1. National Oceanographic Data Center, NOAA. Dataset. doi:10.7289/V5S75D83 [access date]
Access Constraints
  • NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
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Processing Steps
  • 2015-01-20T20:25:06 - NCEI Accession 0125008 v1.1 was published.
Output Datasets
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Processing Steps
  • Parameter or Variable: Dissolved Inorganic Carbon; Abbreviation: DIC; Unit: umol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Sampling instrument: Acid-washed borosilicate glass bottles; CRM manufacturer: Dickson Laboratory, Scripps Institution of Oceanography; Poison name: HgCl2; Poison volume: 20 uL HgCl2 is added to 500 mL samples.; Poison correction: No correction; Method reference: Dickson AG, Sabine CL, Christian JR, editors (2007) Guide to best practices for ocean CO2 measurements: PICES Special Publication 3. 191 p.; Researcher name: Cynthia Peacock, Shallin Busch; Researcher institution: NOAA Pacific Marine Environmental Laboratory, University of Washington's Friday Harbor Laboratory.
  • Parameter or Variable: Total alkalinity; Abbreviation: TA; Unit: umol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Sampling instrument: Acid-washed borosilicate glass bottles; Analyzing instrument: Alkalinity titrator; Cell type (open or closed): Open; Curve fitting method: non-linear least-squares procedure; CRM manufacturer: Dickson Laboratory, Scripps Institution of Oceanography; Poison name: HgCl2; Poison volume: 20 uL HgCl2 is added to 500 mL samples.; Poison correction: No correction; Method reference: Dickson AG, Sabine CL, Christian JR, editors (2007) Guide to best practices for ocean CO2 measurements: PICES Special Publication 3. 191 p.; Researcher name: Cynthia Peacock, Shallin Busch; Researcher institution: NOAA Pacific Marine Environmental Laboratory, University of Washington's Friday Harbor Laboratory.
  • Parameter or Variable: pH; Abbreviation: pH; pH scale: Total; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Sampling instrument: Cuvette; Analyzing instrument: Ocean Optics USB 2000+ Fiber Optic Spectrometer; Temperature of pH measurement: 25C; Replicate information: Measured from system at start and end of each experiment, and sometimes in the middle of an experiment. Measured in the jars where pteropods were reared at the end of each experiment.; At what temperature was pH reported: 12C; Method reference: Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to best practices for ocean CO2 measurements. PICES Special Publication 3, 191 pp.; Researcher name: Shallin Busch; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: pCO2 (fCO2) autonomous; Abbreviation: pCO2; Unit: uatm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Calculated; Detailed sampling and analyzing information: We used DIC and spectrophometric pH measurements to estimate pCO2 with CO2sys version 2.1, using the K1 and K2 constants from Lueker et al., KHSO4 constant from Dickson, [B]T from Uppstrom, and the total pH scale. ; At what temperature was pCO2 reported: 12C; Researcher name: Shallin Busch; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Number of living pteropods at end of experiment; Abbreviation: Live; Unit: Number of living pteropods; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Human; Analyzing instrument: Eye; Duration: 7 day incubation; Detailed sampling and analyzing information: This variable indicates the number of pterpods living at the end of the experiment; Replicate information: Pterpods were sorted categorized as live or dead at the end of each experiment and counted; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Shallin Busch, Tricia Thibodeau; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Number of dead pteropods at end of experiment; Abbreviation: Dead; Unit: Number of dead pteropods; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Human; Analyzing instrument: Eye; Duration: 7 day incubation; Detailed sampling and analyzing information: This variable indicates the number of pterpods dead at the end of the experiment; Replicate information: Pterpods were sorted categorized as live or dead at the end of each experiment and counted; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Shallin Busch, Tricia Thibodeau; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Ratio of live to dead pteropods at end of experiment; Abbreviation: Ratio; Unit: Ratio of live to dead pteropods at end of experiment; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Calculated; Duration: 7 day incubation; Detailed sampling and analyzing information: This variable indicates the ratio of live to dead pteropods at the end of the experiment; Replicate information: Pterpods were sorted categorized as live or dead at the end of each experiment and counted; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Shallin Busch; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Calculated aragonite saturation state; Abbreviation: Arag; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Calculated; Detailed sampling and analyzing information: We used DIC and spectrophometric pH measurements to estimate aragonite saturation state (Omega) with CO2sys version 2.1, using the K1 and K2 constants from Lueker et al., KHSO4 constant from Dickson, [B]T from Uppstrom, and the total pH scale. ; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Shallin Busch; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Score for shell transparency/opaqueness; Abbreviation: Cat1; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Human; Analyzing instrument: Eye; Duration: 7 day incubation; Detailed sampling and analyzing information: We rated the shell condition of pteropods that lived through the seven-day experiment, using the methodology presented in Lischka et al. (2011) with one modification: we immersed samples in 6% sodium hypochlorite for 60 hr to sufficiently clean tissue from the shells. All shells selected for scoring were scored by a single observer, who was blind to treatment conditions, using a Nikon SMZ 745T stereoscope.; Method reference: Lischka S, Budenbender J, Boxhammer T, Riebesell U (2011) Impact of ocean acidification and elevated temperatures on early juveniles of the polar shelled pteropod Limacina helicina: mortality, shell degradation, and shell growth. Biogeosciences 8: 919-932.; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Tricia Thibodeau; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Score for shell transparency/brownness; Abbreviation: Cat2; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Human; Analyzing instrument: Eye; Duration: 7 day incubation; Detailed sampling and analyzing information: We rated the shell condition of pteropods that lived through the seven-day experiment, using the methodology presented in Lischka et al. (2011) with one modification: we immersed samples in 6% sodium hypochlorite for 60 hr to sufficiently clean tissue from the shells. All shells selected for scoring were scored by a single observer, who was blind to treatment conditions, using a Nikon SMZ 745T stereoscope. ; Method reference: Lischka S, Budenbender J, Boxhammer T, Riebesell U (2011) Impact of ocean acidification and elevated temperatures on early juveniles of the polar shelled pteropod Limacina helicina: mortality, shell degradation, and shell growth. Biogeosciences 8: 919-932.; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Tricia Thibodeau; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Score for shell scarred structures; Abbreviation: Cat3; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Human; Analyzing instrument: Eye; Duration: 7 day incubation; Detailed sampling and analyzing information: We rated the shell condition of pteropods that lived through the seven-day experiment, using the methodology presented in Lischka et al. (2011) with one modification: we immersed samples in 6% sodium hypochlorite for 60 hr to sufficiently clean tissue from the shells. All shells selected for scoring were scored by a single observer, who was blind to treatment conditions, using a Nikon SMZ 745T stereoscope. ; Method reference: Lischka S, Budenbender J, Boxhammer T, Riebesell U (2011) Impact of ocean acidification and elevated temperatures on early juveniles of the polar shelled pteropod Limacina helicina: mortality, shell degradation, and shell growth. Biogeosciences 8: 919-932.; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Tricia Thibodeau; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Score for shell corrosion; Abbreviation: Cat4; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Human; Analyzing instrument: Eye; Duration: 7 day incubation; Detailed sampling and analyzing information: We rated the shell condition of pteropods that lived through the seven-day experiment, using the methodology presented in Lischka et al. (2011) with one modification: we immersed samples in 6% sodium hypochlorite for 60 hr to sufficiently clean tissue from the shells. All shells selected for scoring were scored by a single observer, who was blind to treatment conditions, using a Nikon SMZ 745T stereoscope. ; Method reference: Lischka S, Budenbender J, Boxhammer T, Riebesell U (2011) Impact of ocean acidification and elevated temperatures on early juveniles of the polar shelled pteropod Limacina helicina: mortality, shell degradation, and shell growth. Biogeosciences 8: 919-932.; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Tricia Thibodeau; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Score for shell number of perforations ; Abbreviation: Cat5; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Human; Analyzing instrument: Eye; Duration: 7 day incubation; Detailed sampling and analyzing information: We rated the shell condition of pteropods that lived through the seven-day experiment, using the methodology presented in Lischka et al. (2011) with one modification: we immersed samples in 6% sodium hypochlorite for 60 hr to sufficiently clean tissue from the shells. All shells selected for scoring were scored by a single observer, who was blind to treatment conditions, using a Nikon SMZ 745T stereoscope. ; Method reference: Lischka S, Budenbender J, Boxhammer T, Riebesell U (2011) Impact of ocean acidification and elevated temperatures on early juveniles of the polar shelled pteropod Limacina helicina: mortality, shell degradation, and shell growth. Biogeosciences 8: 919-932.; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Tricia Thibodeau; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Sum of scores for shell state (Cat1-Cat5); Abbreviation: Sum; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Calculated; Duration: 7 day incubation; Detailed sampling and analyzing information: We rated the shell condition of pteropods that lived through the seven-day experiment, using the methodology presented in Lischka et al. (2011) with one modification: we immersed samples in 6% sodium hypochlorite for 60 hr to sufficiently clean tissue from the shells. All shells selected for scoring were scored by a single observer, who was blind to treatment conditions, using a Nikon SMZ 745T stereoscope. This measure represents the sum of the 5 characteristics that shells were scored for.; Method reference: Lischka S, Budenbender J, Boxhammer T, Riebesell U (2011) Impact of ocean acidification and elevated temperatures on early juveniles of the polar shelled pteropod Limacina helicina: mortality, shell degradation, and shell growth. Biogeosciences 8: 919-932.; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Tricia Thibodeau; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: Salinity; Abbreviation: Salinity; Unit: psu; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Analyzing instrument: Honeywell conductivity probe ; Duration: 7 day incubation; Replicate information: Measured continuously throughout each experiment; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Michael Maher; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: pH measured by Durafet in experimental system; Abbreviation: Durafet; Unit: total pH; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Analyzing instrument: Honeywell Durafet pH probe; Duration: 7 day incubation; Replicate information: Measured continuously throughout each experiment; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Michael Maher; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
  • Parameter or Variable: pH measured by spectrophotometer; Abbreviation: Spec; Unit: total pH; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Analyzing instrument: Ocean Optics USB 2000+ Fiber Optic Spectrometer; Duration: 7 day incubation; Replicate information: Measured from system at start and end of each experiment, and sometimes in the middle of an experiment. Measured in the jars where pteropods were reared at the end of each experiment.; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Michael Maher; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
Lineage information for: repository
Processing Steps
  • 2015-04-22T00:00:00 - NOAA created the National Centers for Environmental Information (NCEI) by merging NOAA's National Climatic Data Center (NCDC), National Geophysical Data Center (NGDC), and National Oceanographic Data Center (NODC), including the National Coastal Data Development Center (NCDDC), per the Consolidated and Further Continuing Appropriations Act, 2015, Public Law 113-235. NCEI launched publicly on April 22, 2015.
Acquisition Information (collection)
Instrument
  • spectrophotometer
Last Modified: 2018-09-17T20:22:40
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