Processing Steps |
- Parameter or Variable: Dissolved Inorganic Carbon; Abbreviation: DIC; Unit: umol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Sampling instrument: Acid-washed borosilicate glass bottles; CRM manufacturer: Dickson Laboratory, Scripps Institution of Oceanography; Preservation method: HgCl2; Preservative volume: 20 uL HgCl2 is added to 500 mL samples.; Preservative correction: No correction; Method reference: Dickson AG, Sabine CL, Christian JR, editors (2007) Guide to best practices for ocean CO2 measurements: PICES Special Publication 3. 191 p.; Researcher name: Cynthia Peacock, Shallin Busch; Researcher institution: NOAA Pacific Marine Environmental Laboratory, University of Washington's Friday Harbor Laboratory.
- Parameter or Variable: Total alkalinity; Abbreviation: TA; Unit: umol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Sampling instrument: Acid-washed borosilicate glass bottles; Analyzing instrument: Alkalinity titrator; Cell type (open or closed): Open; Curve fitting method: non-linear least-squares procedure; CRM manufacturer: Dickson Laboratory, Scripps Institution of Oceanography; Preservation method: HgCl2; Preservative volume: 20 uL HgCl2 is added to 500 mL samples.; Preservative correction: No correction; Method reference: Dickson AG, Sabine CL, Christian JR, editors (2007) Guide to best practices for ocean CO2 measurements: PICES Special Publication 3. 191 p.; Researcher name: Cynthia Peacock, Shallin Busch; Researcher institution: NOAA Pacific Marine Environmental Laboratory, University of Washington's Friday Harbor Laboratory.
- Parameter or Variable: pH; Abbreviation: pH; pH scale: Total; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Sampling instrument: Cuvette; Analyzing instrument: Ocean Optics USB 2000+ Fiber Optic Spectrometer; Temperature of pH measurement: 25C; Replicate information: Measured from system at start and end of each experiment, and sometimes in the middle of an experiment. Measured in the jars where pteropods were reared at the end of each experiment.; At what temperature was pH reported: 12C; Method reference: Dickson, A.G., Sabine, C.L. and Christian, J.R. (Eds.) 2007. Guide to best practices for ocean CO2 measurements. PICES Special Publication 3, 191 pp.; Researcher name: Shallin Busch; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
- Parameter or Variable: pCO2 (fCO2) autonomous; Abbreviation: pCO2; Unit: uatm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Calculated; Detailed sampling and analyzing information: We used DIC and spectrophometric pH measurements to estimate pCO2 with CO2sys version 2.1, using the K1 and K2 constants from Lueker et al., KHSO4 constant from Dickson, [B]T from Uppstrom, and the total pH scale.; At what temperature was pCO2 reported: 12C; Researcher name: Shallin Busch; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
- Parameter or Variable: Number of living pteropods at end of experiment; Abbreviation: Live; Unit: Number of living pteropods; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Human; Analyzing instrument: Eye; Duration: 7 day incubation; Detailed sampling and analyzing information: This variable indicates the number of pterpods living at the end of the experiment; Replicate information: Pterpods were sorted categorized as live or dead at the end of each experiment and counted; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Shallin Busch, Tricia Thibodeau; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
- Parameter or Variable: Number of dead pteropods at end of experiment; Abbreviation: Dead; Unit: Number of dead pteropods; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Human; Analyzing instrument: Eye; Duration: 7 day incubation; Detailed sampling and analyzing information: This variable indicates the number of pterpods dead at the end of the experiment; Replicate information: Pterpods were sorted categorized as live or dead at the end of each experiment and counted; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Shallin Busch, Tricia Thibodeau; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
- Parameter or Variable: Ratio of live to dead pteropods at end of experiment; Abbreviation: Ratio; Unit: Ratio of live to dead pteropods at end of experiment; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Calculated; Duration: 7 day incubation; Detailed sampling and analyzing information: This variable indicates the ratio of live to dead pteropods at the end of the experiment; Replicate information: Pterpods were sorted categorized as live or dead at the end of each experiment and counted; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Shallin Busch; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
- Parameter or Variable: Calculated aragonite saturation state; Abbreviation: Arag; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Calculated; Detailed sampling and analyzing information: We used DIC and spectrophometric pH measurements to estimate aragonite saturation state (Omega) with CO2sys version 2.1, using the K1 and K2 constants from Lueker et al., KHSO4 constant from Dickson, [B]T from Uppstrom, and the total pH scale.; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Shallin Busch; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
- Parameter or Variable: Score for shell transparency/opaqueness; Abbreviation: Cat1; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Human; Analyzing instrument: Eye; Duration: 7 day incubation; Detailed sampling and analyzing information: We rated the shell condition of pteropods that lived through the seven-day experiment, using the methodology presented in Lischka et al. (2011) with one modification: we immersed samples in 6% sodium hypochlorite for 60 hr to sufficiently clean tissue from the shells. All shells selected for scoring were scored by a single observer, who was blind to treatment conditions, using a Nikon SMZ 745T stereoscope.; Method reference: Lischka S, Budenbender J, Boxhammer T, Riebesell U (2011) Impact of ocean acidification and elevated temperatures on early juveniles of the polar shelled pteropod Limacina helicina: mortality, shell degradation, and shell growth. Biogeosciences 8: 919-932.; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Tricia Thibodeau; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
- Parameter or Variable: Score for shell transparency/brownness; Abbreviation: Cat2; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Human; Analyzing instrument: Eye; Duration: 7 day incubation; Detailed sampling and analyzing information: We rated the shell condition of pteropods that lived through the seven-day experiment, using the methodology presented in Lischka et al. (2011) with one modification: we immersed samples in 6% sodium hypochlorite for 60 hr to sufficiently clean tissue from the shells. All shells selected for scoring were scored by a single observer, who was blind to treatment conditions, using a Nikon SMZ 745T stereoscope.; Method reference: Lischka S, Budenbender J, Boxhammer T, Riebesell U (2011) Impact of ocean acidification and elevated temperatures on early juveniles of the polar shelled pteropod Limacina helicina: mortality, shell degradation, and shell growth. Biogeosciences 8: 919-932.; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Tricia Thibodeau; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
- Parameter or Variable: Score for shell scarred structures; Abbreviation: Cat3; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Human; Analyzing instrument: Eye; Duration: 7 day incubation; Detailed sampling and analyzing information: We rated the shell condition of pteropods that lived through the seven-day experiment, using the methodology presented in Lischka et al. (2011) with one modification: we immersed samples in 6% sodium hypochlorite for 60 hr to sufficiently clean tissue from the shells. All shells selected for scoring were scored by a single observer, who was blind to treatment conditions, using a Nikon SMZ 745T stereoscope.; Method reference: Lischka S, Budenbender J, Boxhammer T, Riebesell U (2011) Impact of ocean acidification and elevated temperatures on early juveniles of the polar shelled pteropod Limacina helicina: mortality, shell degradation, and shell growth. Biogeosciences 8: 919-932.; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Tricia Thibodeau; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
- Parameter or Variable: Score for shell corrosion; Abbreviation: Cat4; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Human; Analyzing instrument: Eye; Duration: 7 day incubation; Detailed sampling and analyzing information: We rated the shell condition of pteropods that lived through the seven-day experiment, using the methodology presented in Lischka et al. (2011) with one modification: we immersed samples in 6% sodium hypochlorite for 60 hr to sufficiently clean tissue from the shells. All shells selected for scoring were scored by a single observer, who was blind to treatment conditions, using a Nikon SMZ 745T stereoscope.; Method reference: Lischka S, Budenbender J, Boxhammer T, Riebesell U (2011) Impact of ocean acidification and elevated temperatures on early juveniles of the polar shelled pteropod Limacina helicina: mortality, shell degradation, and shell growth. Biogeosciences 8: 919-932.; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Tricia Thibodeau; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
- Parameter or Variable: Score for shell number of perforations; Abbreviation: Cat5; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Measured; Sampling instrument: Human; Analyzing instrument: Eye; Duration: 7 day incubation; Detailed sampling and analyzing information: We rated the shell condition of pteropods that lived through the seven-day experiment, using the methodology presented in Lischka et al. (2011) with one modification: we immersed samples in 6% sodium hypochlorite for 60 hr to sufficiently clean tissue from the shells. All shells selected for scoring were scored by a single observer, who was blind to treatment conditions, using a Nikon SMZ 745T stereoscope.; Method reference: Lischka S, Budenbender J, Boxhammer T, Riebesell U (2011) Impact of ocean acidification and elevated temperatures on early juveniles of the polar shelled pteropod Limacina helicina: mortality, shell degradation, and shell growth. Biogeosciences 8: 919-932.; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Tricia Thibodeau; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
- Parameter or Variable: Sum of scores for shell state (Cat1-Cat5); Abbreviation: Sum; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Response variable; Measured or calculated: Calculated; Duration: 7 day incubation; Detailed sampling and analyzing information: We rated the shell condition of pteropods that lived through the seven-day experiment, using the methodology presented in Lischka et al. (2011) with one modification: we immersed samples in 6% sodium hypochlorite for 60 hr to sufficiently clean tissue from the shells. All shells selected for scoring were scored by a single observer, who was blind to treatment conditions, using a Nikon SMZ 745T stereoscope. This measure represents the sum of the 5 characteristics that shells were scored for.; Method reference: Lischka S, Budenbender J, Boxhammer T, Riebesell U (2011) Impact of ocean acidification and elevated temperatures on early juveniles of the polar shelled pteropod Limacina helicina: mortality, shell degradation, and shell growth. Biogeosciences 8: 919-932.; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Tricia Thibodeau; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
- Parameter or Variable: Salinity; Abbreviation: Salinity; Unit: psu; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Analyzing instrument: Honeywell conductivity probe; Duration: 7 day incubation; Replicate information: Measured continuously throughout each experiment; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Michael Maher; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
- Parameter or Variable: pH measured by Durafet in experimental system; Abbreviation: Durafet; Unit: total pH; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Analyzing instrument: Honeywell Durafet pH probe; Duration: 7 day incubation; Replicate information: Measured continuously throughout each experiment; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Michael Maher; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration.
- Parameter or Variable: pH measured by spectrophotometer; Abbreviation: Spec; Unit: total pH; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Analyzing instrument: Ocean Optics USB 2000+ Fiber Optic Spectrometer; Duration: 7 day incubation; Replicate information: Measured from system at start and end of each experiment, and sometimes in the middle of an experiment. Measured in the jars where pteropods were reared at the end of each experiment.; Biological subject: Pteropod; Species ID: Limacina helicina; Researcher name: Michael Maher; Researcher institution: Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration .
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