Effects of elevated CO2 levels on eggs and larvae of a North Pacific flatfish (northern rock sole, Lepidopsetta polyxystra) from laboratory experiment studies from 2012-02-01 to 2013-09-30 (NCEI Accession 0136906)
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title: Effects of elevated CO2 levels on eggs and larvae of a North Pacific flatfish (northern rock sole, Lepidopsetta polyxystra) from laboratory experiment studies from 2012-02-01 to 2013-09-30 (NCEI Accession 0136906)
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abstract: This dataset contains the results of a laboratory experiment study to understand the effect of ocean acidification on eggs and larvae of northern rock sole, Lepidopsetta polyxystra. The Bering Sea and Gulf of Alaska support a number of commercially important flatfish fisheries. These high latitude ecosystems are predicted to be most immediately impacted by ongoing ocean acidification but the range of responses by commercial fishery species has yet to be fully explored. In this study we examined the growth responses of northern rock sole (Lepidopsetta polyxystra) eggs and larvae across a range of CO2 levels (ambient to 1500 uatm) to evaluate the potential sensitivity to ocean acidification. Laboratory-spawned eggs and larvae were reared at 8 degrees Celsius in a flow-through culture system in which CO2 levels were maintained via computer-controlled injection of CO2 into a seawater conditioning tank. Overall, we observed only minor effects of elevated CO2 level on sizes of northern rock sole larvae. Size at hatch differed among offspring from four different females, but there was no significant effect of CO2 level on egg survival or size at hatch. In three separate larval growth trials, there was little effect of CO2 level on growth rates through the first 28 days post-hatch (DPH). However, in the one trial extended to 60 DPH, fish reared at the highest CO2 level had lower condition factors after 28 DPH suggesting that larvae undergoing metamorphosis may be more sensitive to environmental hypercapnia than earlier pre-flexion stages. These results suggest that while early life stages of northern rock sole are less sensitive to ocean acidification than previously examined flatfishes, they may be more sensitive to elevated CO2 levels than a previously studied gadid with a similar geographic range.
purpose: To study how hatch size and larval growth of northern rock sole are affected by ocean acidification.
credit: Funding Information: NOAA Ocean Acidification Program (Effects of Ocean Acidification on Early Life Stages of Alaska Fishery Resources, OAPFY13.03.AFSC.004)
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title: NOAA National Centers for Environmental Information (2022). Ocean Carbon and Acidification Data System (OCADS). NOAA National Centers for Environmental Information. https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system
date: (CI_Date)
date: 2022-04
dateType: (CI_DateTypeCode) publication
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contactInfo: (CI_Contact)
onlineResource: (CI_OnlineResource)
linkage: https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system
protocol: HTTPS
name: https://www.ncei.noaa.gov/products/ocean-carbon-acidification-data-system
description: OCADS website
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1730 NOAA National Centers for Environmental Information
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Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1730 US DOC; NOAA; NESDIS; National Centers for Environmental Information (NCEI)
role: (CI_RoleCode) publisher
presentationForm: (CI_PresentationFormCode) documentDigital
otherCitationDetails: [accessed 2024-02-08]
associationType: (DS_AssociationTypeCode) crossReference
initiativeType: (DS_InitiativeTypeCode) program
language:
LanguageCode: eng; USA
characterSet: (MD_CharacterSetCode) utf8
topicCategory: (MD_TopicCategoryCode) environment
topicCategory: (MD_TopicCategoryCode) oceans
topicCategory: (MD_TopicCategoryCode) biota
extent: (EX_Extent)
geographicElement: (EX_GeographicBoundingBox)
westBoundLongitude: -122.76
eastBoundLongitude: -122.76
southBoundLatitude: 48.135
northBoundLatitude: 48.135
temporalElement: (EX_TemporalExtent)
extent:
TimePeriod:
beginPosition: 2012-02-01
endPosition: 2013-09-30
supplementalInformation: PRINCIPAL INVESTIGATORS: Hurst, Thomas P.; Mathis, Jeremy T.; and Laurel, Benjamin J. FUNDING AGENCY: NOAA's Ocean Acidification Program (OAP). PROJECT TITLE: Effects of Ocean Acidification on Early Life Stages of Alaska Fishery Resources: Walleye Pollock. PROJECT ID: OAPFY13.03.AFSC.004.
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distributionInfo: (MD_Distribution)
distributor: (MD_Distributor)
distributorContact: (CI_ResponsibleParty)
organisationName:
Anchor: https://www.ncei.noaa.gov/archive/archive-management-system/OAS/bin/prd/jquery/institution/details/1730 NOAA National Centers for Environmental Information
contactInfo: (CI_Contact)
phone: (CI_Telephone)
voice: +1-301-713-3277
address: (CI_Address)
electronicMailAddress: NCEI.Info@noaa.gov
role: (CI_RoleCode) pointOfContact
distributionOrderProcess: (MD_StandardOrderProcess)
fees: In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
orderingInstructions: Contact NCEI for other distribution options and instructions.
distributorFormat: (MD_Format)
name: Originator data format
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linkage: https://www.ncei.noaa.gov/data/oceans/ncei/ocads/metadata/0136906.html
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name: Project Metadata
description: Navigate directly to the URL for a descriptive web page with download links.
function: (CI_OnLineFunctionCode) information
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linkage: https://doi.org/10.7289/v5zw1hzw
protocol: HTTPS
applicationProfile: Web browser
name: NCEI Dataset Landing Page
description: Navigate directly to the URL for a descriptive web page with download links.
function: (CI_OnLineFunctionCode) information
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linkage: https://www.ncei.noaa.gov/archive/accession/oas/136906
protocol: HTTPS
applicationProfile: Web browser
name: Descriptive Information
description: Navigate directly to the URL for a descriptive web page with download links.
function: (CI_OnLineFunctionCode) information
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linkage: https://www.ncei.noaa.gov/archive/accession/download/136906
protocol: HTTPS
applicationProfile: Web browser
name: HTTPS
description: Navigate directly to the URL for data access and direct download.
function: (CI_OnLineFunctionCode) download
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linkage: ftp://ftp-oceans.ncei.noaa.gov/nodc/archive/arc0081/0136906/
protocol: FTP
applicationProfile: Any FTP client
name: FTP
description: These data are available through the File Transfer Protocol (FTP). FTP is no longer supported by most internet browsers. You may copy and paste the FTP link to the data into an FTP client (e.g., FileZilla or WinSCP).
function: (CI_OnLineFunctionCode) download
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dataQualityInfo: (DQ_DataQuality)
scope: (DQ_Scope)
level: (MD_ScopeCode) dataset
lineage: (LI_Lineage)
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description: NCEI Accession 0136906 v1.1 was published.
dateTime:
DateTime: 2015-12-02T16:40:08Z
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title: NCEI Accession 0136906 v1.1
date: (CI_Date)
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dateType: (CI_DateTypeCode) publication
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contactInfo: (CI_Contact)
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linkage: https://www.ncei.noaa.gov/archive/accession/0136906/1.1
protocol: HTTPS
name: NCEI Accession 0136906 v1.1
description: published 2015-12-02T16:40:08Z
function: (CI_OnLineFunctionCode) download
role: (inapplicable)
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dataQualityInfo: (DQ_DataQuality)
scope: (DQ_Scope)
level: (MD_ScopeCode) dataset
lineage: (LI_Lineage)
processStep: (LE_ProcessStep)
description: Parameter or Variable: Dissolved Inorganic Carbon; Abbreviation: DIC; Unit: umol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Sampling instrument: Siphoning tube; Analyzing instrument: VINDTA 3C (Versatile Instrument for the Determination of dissolved inorganic carbon and Total Alkalinity) coupled to a UIC 5014 coulometer.; Preservation method: saturated HgCl2; Preservative correction: no correction.; Uncertainty: less than 0.05%; Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error.; Researcher name: Jeremy T. Mathis; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Total alkalinity; Abbreviation: TA; Unit: umol/kg; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Measured; Sampling instrument: Siphoning tube; Analyzing instrument: Alkalinity titrator; Preservation method: saturated HgCl2; Preservative correction: no correction.; Uncertainty: less than 0.05%; Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error.; Researcher name: Jeremy T. Mathis; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks.
processStep: (LE_ProcessStep)
description: Parameter or Variable: pH; Abbreviation: calcpH; pH scale: Seawater; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Calculated; Detailed sampling and analyzing information: calculated from DIC, TA, temperature, and salinity; At what temperature was pH reported: 8.0; Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error.; Method reference: Lewis and Wallace 1995. Basic programs for the CO2 system in seawater. Brookhaven National Laboratory. BNL-61827; Researcher name: Jeremy T. Mathis; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks.
processStep: (LE_ProcessStep)
description: Parameter or Variable: pCO2 (fCO2) autonomous; Abbreviation: calcCO2; Unit: uatm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: Calculated; Detailed sampling and analyzing information: calculated from DIC, TA, temperature, and salinity; At what temperature was pCO2 reported: 8.0; Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error.; Method reference: Lewis and Wallace 1995. Basic programs for the CO2 system in seawater. Brookhaven National Laboratory. BNL-61827; Researcher name: Jeremy T. Mathis; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Water temperature; Abbreviation: Temp; Unit: degrees centigrade; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: measured; Sampling instrument: various; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Salinity; Abbreviation: Sal; Unit: parts per thousand; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: measured; Sampling instrument: Yellow Springs International meter - Model YSI85; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Code for egg or larval exposure experiment; Abbreviation: EXPstage; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: days organisms was under manipulated CO2 conditions; Abbreviation: ExposureDays; Unit: days; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: nominal identifier of specific egg batch upon which experiment was performed; Abbreviation: EggBatch; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: categorical identifier of treatment in CO2 experiment; Abbreviation: TRTlabel; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: sampling date; Abbreviation: date; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: time of day measurement taken; Abbreviation: time; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Laboratory identification of sampled tank; Abbreviation: Tank; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Calcite saturation state; Abbreviation: CAsat; Unit: omega; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: calculated; Detailed sampling and analyzing information: calculated using CO2sys program; Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error.; Researcher name: Jeremy T. Mathis; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Data quality flag for CO2 system measures; Abbreviation: h20DQ; Observation type: Laboratory experiment; Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error.; Researcher name: Jeremy T. Mathis; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Aragonite saturation state; Abbreviation: ARsat; Unit: omega; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Measured or calculated: calculated; Detailed sampling and analyzing information: calculated using CO2sys program; Quality flag convention: "h2oDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier/estimated; 3=known error.; Researcher name: Jeremy T. Mathis; Researcher institution: Ocean Acidification Research Center at the University of Alaska at Fairbanks.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Replicate number within treatment; Abbreviation: replicate; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Mean standard length; Abbreviation: SLmean; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 20 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 20 fish were measured from each treatment replicate on each sampling date; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Standard deviation of standard length; Abbreviation: SLsed; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Duration: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 20 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 20 fish were measured from each treatment replicate on each sampling date; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Mean body height (myotome height); Abbreviation: MHmean; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 20 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 20 fish were measured from each treatment replicate on each sampling date; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Standard deviation of body height; Abbreviation: HTsd; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 20 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 20 fish were measured from each treatment replicate on each sampling date; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Mean yolk area; Abbreviation: YAmean; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 20 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 20 fish were measured from each treatment replicate on each sampling date; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Standard deviation of yolk area; Abbreviation: YAsd; Unit: mm; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 20 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 20 fish were measured from each treatment replicate on each sampling date; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Mean body condition - expressed as absolute deviation from from ontogenetic relationship between SL and MH; Abbreviation: MHdevmean; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: calculated; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 20 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 20 fish were measured from each treatment replicate on each sampling date; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Standard deviation of body condition metric; Abbreviation: MHdevsd; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: measured; Sampling instrument: dissecting microscope; Analyzing instrument: ImagePro image analysis system; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 20 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 20 fish were measured from each treatment replicate on each sampling date; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Number of fish measured for size metrics; Abbreviation: size_n; Replicate information: Up to 20 fish were measured from each treatment replicate on each sampling date; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Mean body condition - expressed as absolute deviation from from ontogenetic relationship between SL and MH; Abbreviation: MHdevPer; Observation type: Laboratory experiment; Sampling instrument: calculated; Detailed sampling and analyzing information: Larvae were sampled and measured at specified intervals in the experiment. Each sampling, up to 20 larvae from each replicate tank were digitally photographed under a dissecting microscope. An image analysis system was used to measure fish from the photographs.; Replicate information: Up to 20 fish were measured from each treatment replicate on each sampling date; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Standard deviation of body condition metric; Abbreviation: MHdevPsd; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: response variable; Measured or calculated: measured; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: number of independent dry weight samples; Abbreviation: DWn; Observation type: Laboratory experiment; In-situ / Manipulation / Response variable: Manipulation condition; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: mean dry weight per fish; Abbreviation: DWmean; Observation type: Laboratory experiment; Measured or calculated: Measured; Sampling instrument: Sartorius Microbalance ME5; Detailed sampling and analyzing information: Fish were rinsed in ammonium formate and dried at 50degrees Celsius for at least 48 hours prior to measurement; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: standard deviation of mean dry weight per fish; Abbreviation: DWsd; Observation type: Laboratory experiment; Measured or calculated: Measured; Sampling instrument: Sartorius Microbalance ME5; Detailed sampling and analyzing information: Fish were rinsed in ammonium formate and dried at 50degrees Celsius for at least 48 hours prior to measurement; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: predicted mean dryweight for fish in that sample based on ontegenetic relationship between SL and DW; Abbreviation: DWpred; Observation type: Laboratory experiment; Measured or calculated: calculated; Detailed sampling and analyzing information: Fish were rinsed in ammonium formate and dried at 50degrees Celsius for at least 48 hours prior to measurement; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center.
processStep: (LE_ProcessStep)
description: Parameter or Variable: Mean body condition - expressed as absolute deviation from from ontogenetic relationship between SL and DW; Abbreviation: DWdev; Observation type: Laboratory experiment; Measured or calculated: calculated; Detailed sampling and analyzing information: Fish were rinsed in ammonium formate and dried at 50degrees Celsius for at least 48 hours prior to measurement; Quality flag convention: "hatchDQ" reflects reliabiltity of data: 1=good; 2=questionable/outlier; 3=known error.; Biological subject: northern rock sole; Species ID: Lepidopsetta polyxystra; Researcher name: Thomas P. Hurst; Researcher institution: NOAA Alaska Fisheries Science Center .
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metadataMaintenance: (MD_MaintenanceInformation)
maintenanceAndUpdateFrequency: (MD_MaintenanceFrequencyCode) asNeeded
maintenanceNote: Metadata are developed, maintained and distributed by NCEI. Updates are performed as needed to maintain currentness.
contact: (CI_ResponsibleParty)
organisationName: NOAA National Centers for Environmental Information
role: (CI_RoleCode) custodian
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acquisitionInformation: (MI_AcquisitionInformation)
instrument: (MI_Instrument)
identifier: (MD_Identifier)
code: coulometer for DIC measurement
type: coulometer for DIC measurement
description: measures dissolved inorganic carbon (DIC) A known amount of seawater is dispensed into a stripping chamber where it is acidified and purged with an inert gas. The amount of CO2 in the resulting gas stream is determined by absorbing the CO2 in an absorbent. The pH of the solution is monitored by measuring the transmittance .
instrument: (MI_Instrument)
identifier: (MD_Identifier)
code: microscope
type: microscope
description: instrument is used in lab analyses A microscope is an instrument used to see objects that are too small for the naked eye.
instrument: (MI_Instrument)
identifier: (MD_Identifier)
code: multi-parameter water quality sensor
type: multi-parameter water quality sensor
description: used to measure temperature, salinity, dissolved oxygen, and other parameters Instrument used to measure temperature, salinity, dissolved oxygen, and other parameters, often used to measure water quality parameters. Can be a hand-held instrument or a sonde for unattended monitoring. Often used in coastal water monitoring stations. YSI is the preeminent brand (as of 2024).
instrument: (MI_Instrument)
identifier: (MD_Identifier)
code: titrator
type: titrator
description: titrator
