PAM values and coral physiological measures in response to DIN treatments from 2015-07-01 to 2016-01-30 (NCEI Accession 0145017)
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title: PAM values and coral physiological measures in response to DIN treatments from 2015-07-01 to 2016-01-30 (NCEI Accession 0145017)
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abstract: The scope of the proposed work includes a six-month laboratory dose response experiment to determine the effects of environmentally-realistic levels of DIN on coral health and function. Plugs or branch tips of four species of Atlantic scleractinian corals, Porites astreoides, Porites divaricata, Montastraea cavernosa, and Siderastrea siderea were exposed for six months to five concentrations of nitrate. Four independent replicates of each elevated nitrogen concentration was maintained by addition of a nitrate solution to dose water. To link coral physiological performance to exposure over time, the maximum quantum yield of PSII photochemistry of each coral was monitored bi monthly with a submersible pulse amplitude modulated fluorometer (PAM). To assess growth rate and calcification response, buoyant weight measurements will be utilized. To quantify tissue condition, and zooxanthellar density, 25% of each coral was fixed for histological analysis at the end of the experimental period. These metrics are used to identify cause and effect relationships and specific responses associated with quantified levels of nitrate.
purpose: Land-based sources of pollution are a pervasive threat in southeast Florida. This study addresses the effects of environmentally-relevant nutrient and temperature levels on four species of Atlantic scleractinian corals during a six-month tank experiment. Coral health will be assessed by growth rates, PAM fluorometry, and histological analyses of tissue and zooxanthellae condition. These metrics will identify sub-lethal responses and the cause and effect relationships associated with quantified levels of nutrient and temperature exposure. The project will equip resource managers with experimentally determined linkages between coral health and specific nutrient thresholds for planning and implementing LBSP action strategies.
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supplementalInformation: Land-based sources of pollution are a pervasive threat in southeast Florida. This study addresses the effects of environmentally-relevant nutrient and temperature levels on four species of Atlantic scleractinian corals during a six-month tank experiment. Coral health will be assessed by growth rates, PAM fluorometry, and histological analyses of tissue and zooxanthellae condition. These metrics will identify sub-lethal responses and the cause and effect relationships associated with quantified levels of nutrient and temperature exposure. The project will equip resource managers with experimentally determined linkages between coral health and specific nutrient thresholds for planning and implementing LBSP action strategies. Submission Package ID: EYLC2M
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