Effects of Hydrogen Sulfide (H2S) on Z. marina seedlings, seed germination and shoot density from 2013-01-16 to 2015-09-11 (NCEI Accession 0156588)

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Multiple experiments were conducted to determine the effects hydrogen sulfide had on seedlings and seed germination in the seagrass Zostera marina. One study identified lethal limits of H2S for Z. marina seedlings, as well as identified ranges in which Z. marina seedlings are unaffected by sulfide levels. It was determined that Z. marina seedlings’ growth rates are stunted by H2S levels. Another study identified if anoxia, salinity, sulfide, time the seeds were picked or location the seeds were picked from affected seed germination rates or acted as germination cues. Germination rates for each treatment at each measurement were calculated by dividing the number of germinated seeds by the number of total seeds in each vial at each timepoint. A third seed study identified the best storage conditions for Z. marina seeds to maximize seed viability and germination rates, as well as identified possible germination cues for Z. marina seeds. Various seed storage conditions included fridge storage vs sea table storage, storage in mud vs sandy sediment vs seawater, light vs dark conditions and anoxic vs oxygenated seawater. The objectives of a fourth experiment were to census the density of Z. marina shoots along a transect in Padilla bay. By manipulating sediment conditions among some of the plots, we measured how organic matter in sediment is converted to sulfide and how that sulfide affects eelgrass shoot density. To do so blocks of agar and 100g of sucrose were prepared and each treatment plot received half of an agar block, placed beneath the sediment, every two weeks. Control plots had the sediment manipulated in the same way but without adding organic matter under the sediment. At each two week interval, pore water was sipped and measured for salinity, pH, and total sulfide content at each plot, the sediment temperature of each plot was taken, and the number of vegetative and flowering shoots at each plot was recorded.
  • Cite as: Adamczyk, Michael; Monreal, Victoria; Tran, Tyler; Yang, Sylvia; Papacjik, Phillip; Blatt, Ari; Woodrich, Dan; Mayer, Miles; Ades, Kate; Ciesielski, Melissa; Tripp, Laura; Simpson, Alexandra; Shull, David (2016). Effects of Hydrogen Sulfide (H2S) on Z. marina seedlings, seed germination and shoot density from 2013-01-16 to 2015-09-11 (NCEI Accession 0156588). Version 1.1. NOAA National Centers for Environmental Information. Dataset. [access date]
gov.noaa.nodc:0156588
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Distributor DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
301-713-3277
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DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
301-713-3277
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Time Period 2013-01-16 to 2015-09-11
Spatial Bounding Box Coordinates
N: 48.5
S: 46.62
E: -122.48
W: -124.04
Spatial Coverage Map
General Documentation
Publication Dates
  • publication: 2016-10-20
Edition 1.1
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Data Update Frequency As needed
Supplemental Information
Submission Package ID: 7C214R
Purpose Basic research
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Adamczyk, Michael; Monreal, Victoria; Tran, Tyler; Yang, Sylvia; Papacjik, Phillip; Blatt, Ari; Woodrich, Dan; Mayer, Miles; Ades, Kate; Ciesielski, Melissa; Tripp, Laura; Simpson, Alexandra; Shull, David (2016). Effects of Hydrogen Sulfide (H2S) on Z. marina seedlings, seed germination and shoot density from 2013-01-16 to 2015-09-11 (NCEI Accession 0156588). Version 1.1. NOAA National Centers for Environmental Information. Dataset. [access date]
Cited Authors
  • Adamczyk, Michael
  • Monreal, Victoria
  • Tran, Tyler
  • Yang, Sylvia
  • Papacjik, Phillip
  • Blatt, Ari
  • Woodrich, Dan
  • Mayer, Miles
  • Ades, Kate
  • Ciesielski, Melissa
  • Tripp, Laura
  • Simpson, Alexandra
  • Shull, David
Principal Investigators
  • David Shull
Collaborators
  • Sylvia Yang
    Western Washington University (WWU)
  • Michael Adamczyk
  • Phillip Papacjik
  • Ari Blatt
  • Dan Woodrich
  • Miles Mayer
  • Kate Ades
  • Victoria Monreal
  • Melissa Ciesielski
  • Tyler Tran
  • Laura Tripp
  • Alexandra Simpson
Contributors
Resource Providers
Publishers
Acknowledgments
  • Related Funding Agency: Western Washington University
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
Global Change Master Directory (GCMD) Science Keywords
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH
  • EARTH SCIENCE > OCEANS > SALINITY/DENSITY > SALINITY
Provider Keywords
  • Plant Viability
Data Center keywords Global Change Master Directory (GCMD) Data Center Keywords
  • DOC/NOAA/NESDIS/NODC > National Oceanographic Data Center, NESDIS, NOAA, U.S. Department of Commerce
  • DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS
Instrument keywords NODC INSTRUMENT TYPES THESAURUS Provider Instruments
  • Glass thermometer
  • ROSS pH electrode
  • Thermoscientific Orion Star A214 pH/ISE meter
  • Vista Series Instruments A366ATC portable refractometer
Place keywords NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords
  • OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN
Provider Location Keywords
  • Padilla Bay, WA
  • Samish Bay, WA
  • Ship Harbor, WA
  • Willapa Bay, WA
Project keywords Provider Project Names
  • Eelgrass-Sulfide Project
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Adamczyk, Michael; Monreal, Victoria; Tran, Tyler; Yang, Sylvia; Papacjik, Phillip; Blatt, Ari; Woodrich, Dan; Mayer, Miles; Ades, Kate; Ciesielski, Melissa; Tripp, Laura; Simpson, Alexandra; Shull, David (2016). Effects of Hydrogen Sulfide (H2S) on Z. marina seedlings, seed germination and shoot density from 2013-01-16 to 2015-09-11 (NCEI Accession 0156588). Version 1.1. NOAA National Centers for Environmental Information. Dataset. [access date]
Access Constraints
  • NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
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Lineage information for: dataset
Processing Steps
  • 2016-10-20T14:28:43 - NCEI Accession 0156588 v1.1 was published.
Output Datasets
Lineage information for: dataset
Processing Steps
  • Data Type: Hydrogen Sulfide (H2S) (measured); Units: millimole; Observation Type: laboratory analysis; Sampling Instrument: Thermoscientific Orion Star A214 pH/ISE meter; Sampling and Analyzing Method: Pore water sippers: capillary tubing with a syringe needle cap attached to one end with marine sealant. A hot needle was used to poke rows of small holes in the plastic cap. A septa was placed on the other end of the capillary tubing. The capillary tubing was marked with tape 3 cm above the holes that were poked. By pushing the tube, holes first into the sediment to the tape, we were able to extract pore water from a 3cm depth, with a needle and syringe through the septa, without oxygenating the pore water. Pore water is then mixed with a sulfide antioxidant buffer and measured with an ISE probe.
  • Data Type: Plant Viability (measured); Units: count; Observation Type: laboratory analysis; Sampling Instrument: laboratory analysis; Sampling and Analyzing Method: Length of shoots, width of shoots, number of leaves, number of branches, seed germination, shoot color, density, flowering and vegetative shoot counts. Seeds which had a radicle fully extending from the seed (only attached to the seed at the base of the radicle) were considered germinated. At the end of the experiment, seeds were taken out of each vial with forceps. If the seed squished apart it was deemed unviable. If a shoot was present on germinated seed, a determination of shoot color was made.
  • Data Type: TEMPERATURE - SEDIMENT (measured); Units: C; Observation Type: in situ; Sampling Instrument: Glass thermometer.
  • Data Type: pH (measured); Units: pH; Observation Type: laboratory analysis; Sampling Instrument: ROSS pH electrode.
  • Data Type: SALINITY (measured); Units: ppt; Observation Type: laboratory analysis; Sampling Instrument: Vista Series Instruments A366ATC portable refractometer.
Acquisition Information (collection)
Instrument
  • laboratory analysis
Last Modified: 2018-11-05T15:31:12
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