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Raw light data, coral host genotypic information and zooxanthellae type and relative abundance from coral reefs in the Florida Keys 2015-09-21 to 2016-05-26 (NCEI Accession 0175572)


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            title:  Raw light data, coral host genotypic information and zooxanthellae type and relative abundance from coral reefs in the Florida Keys 2015-09-21 to 2016-05-26 (NCEI Accession 0175572)
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                date:  2018-09-11
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        abstract:  Several parameters were used to assess coral bleaching in the Florida Keys as part of NOAA’s Coral Reef Conservation Program (CRCP). The data described in this package are light, seawater pH, and coral symbiont assemblage data collected from the upper and lower Florida Keys. Light data was collected using a PAR sensor which measures light as photosynthetically active radiation (PAR, umol m-2 s-1). Seawater pH was collected using a SAMI pH sensor or a SeaBird SeaFET pH sensor. These sensors were located at the same sites as the PAR sensors and recorded pH hourly. During peak bleaching (22-25 September 2015), 20 colonies of O. faveolata were sampled with a hammer and chisel from 10 sites (n=200 total coral samples). All corals were sampled from the top of the colony given that symbiont types can change with colony orientation. A small subsample was immediately preserved in 95% ethanol for genetic analysis. Genomic DNA was extracted using the organic extraction protocol described in Rowan and Power (1991). Quantitative PCR (qPCR) assays were used to understand patterns of algal symbiont community structure to detect the presence of background symbiont types. Zooxanthellae type and abundance data are described in this package to understand the relationship between O. faveolata and Symbiodinium to assess coral bleaching responses. Symbiont assemblages in the data set are described by clades A, B, C and D since O. faveolata has been shown to associate with members from all these clades.
        purpose:  This dataset is available to the public for a wide variety of uses including scientific research and analysis.
        credit:  Related Funding Agency: NOAA Coral Reef Conservation Program
        credit:  Related Funding Agency: National Oceanic and Atmospheric Administration
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                    name:  Molecular Ecology Resources
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                title:  Landscape ecology of algal symbionts creates variation in episodes of coral bleaching
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                title:  Molecular genetic identification of symbiotic dinoflagellates (zooxanthellae)
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                    code: https://doi.org/10.3354/meps071065
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                    name:  Marine Ecology Progress Series
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        supplementalInformation:  Submission Package ID: M2EAWC
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                fees:  In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
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                description:  NCEI Accession 0175572 v1.1 was published.
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                description:  Parameter or Variable: PHOTOSYNTHETIC ACTIVE RADIATION (PAR) (measured); Units: umol m-2 s-1; Observation Category: in situ; Sampling Instrument: sensor; Sampling and Analyzing Method: Photosynthetically active radiation (PAR, 400-700nm) was measured hourly using SeaBIRD EcoPAR.
            processStep:  (LE_ProcessStep)
                description:  Parameter or Variable: pH (measured); Units: pH; Observation Category: in situ; Sampling Instrument: sensor; Sampling and Analyzing Method: Seawater pH was measured hourly. Two instruments were used. (1) SAMI-pH (2) SeaFET-pH sensor.
            processStep:  (LE_ProcessStep)
                description:  Parameter or Variable: DNA (measured); Units: count; Observation Category: laboratory analysis; Sampling Instrument: PCR machine; Sampling and Analyzing Method: Quantitative PCR (qPCR) assays were used to understand patterns of algal symbiont community structure to detect the presence of background symbiont types. Zooxanthallae type and abundance data are described in this package to understand the relationship between O. faveolata and Symbiodinium to assess coral bleaching responses. Symbiont assemblages in the data set are described by clades A, B, C and D since O. faveolata has been shown to associate with members from all these clades..
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                code:  PAR Sensor
            type:  PAR Sensor
            description:  Photosynthetically Active Radiation Sensor The PAR sensor measures global solar radiation from 400 to 700 nm, which approximates the spectral band active in photosynthesis. The response of the instrument falls sharply to zero on either side of this band, and between 400 and 670 nm it increases monotonically from about 50% to 100%. There is no protective glass dome over the receiving surface. Irradiance from the sun passes through a small white visible bandpass filter/diffuser in the shape of a horizontal disk at the top of the instrument. Within the instrument radiation is directed through a series of colored glass filters and onto a silicon photodiode detector. The factory-supplied calibration converts the signal from the detector to a flux of photons in micro-mole (of photons) s-1 m-2. (One micro-mole equals 6.022 E17 photons.) For the solar spectrum, these units may be converted to watts per square meter by dividing by 4.6. (http://www.srrb.noaa.gov/instrument/par.htm)
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                code:  PCR machine
            type:  PCR machine
            description:  thermal cycler, thermocycler, PCR machine, DNA amplifier The thermal cycler (also known as a thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR). https://en.wikipedia.org/wiki/Thermal_cycler, 2017-05-02.
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                code:  pH sensor
            type:  pH sensor
            description:  generic meter used to measure pH Any of a class of instruments used to quantify pH in a water or other type of sample.