Seagrass canopy height, water depth, chlorophyll-a concentration and other plant and water quality indicators in Coastal Waters of Texas (NCEI Accession 0181898)

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This dataset contains raw tier 2 sampling data starting from 2011 for Texas coastal waters related to seagrasses. Parameters measured include chlorophyll a concentration, water depth, dissolved oxygen, light attenuation, pH, salinity, secchi depth, water temperature, total suspended solids, seagrass canopy height, and seagrass percent cover. Typical species represented within the data include Halodule wrightii, Thalassia testudinum, Syringodium filiforme, Halophila johnsonii, and Ruppia maritima. Sampling takes place at 567 permanent stations in Aransas and Redfish Bays, Corpus Christi Bay, and Upper and Lower Laguna Madre. Latitudes and longitudes for station locations are based on the NAD83 datum. There was limited sampling in 2016. This dataset also includes opportunistic sampling in Little Bay, San Antonio Bay, and Galveston Bay, as well as analytically measured tissue content of carbon, nitrogen, and phosphorus in Halodule wrightii and Thalassia testudinum plant samples collected from Aransas and Redfish Bays, Corpus Christi Bay, and the Laguna Madre along with stable isotope data from those samples. Data are provided in CSV format.
  • Cite as: Dunton, Ken; Jackson, Kim; Wilson, Sara; Congdon, Victoria; Cuddy, Meaghan; Hall, Wayne; Becker, Madison; Meiman, Joe; Whiteaker, Tim; Bohannon, Patrick; Grubbs, Faye; Hobson, Cindy; The University of Texas at Austin (2018). Seagrass canopy height, water depth, chlorophyll-a concentration and other plant and water quality indicators in Coastal Waters of Texas (NCEI Accession 0181898). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://accession.nodc.noaa.gov/0181898. Accessed [date].
gov.noaa.nodc:0181898
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Distributor DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
301-713-3277
NCEI.Info@noaa.gov
Dataset Point of Contact Information Services
DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
301-713-3277
NCEI.Info@noaa.gov
Time Period 2011-07-15 to 2017-11-17
Spatial Bounding Box Coordinates
N: 28.434
S: 26
E: -96.396
W: -97.508
Spatial Coverage Map
General Documentation
Associated Resources
Publication Dates
  • publication: 2018-12-19
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Supplemental Information
Submission Package ID: 722X0X

Initial areas of seagrass coverage used for determining station locations were based on the 2004/2007 NOAA Benthic Habitat Assessment.
Purpose These data were collected to support the study of a Texas Seagrass Monitoring Plan implemented using Tier 2 protocols, which are intended to provide rapid assessments of hydrography, seagrass areal coverage, species distributions and plant physiological conditions. These data can be used to evaluate spatial characteristics of seagrass habitat quality and identify regions exhibiting seagrass decline.
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Dunton, Ken; Jackson, Kim; Wilson, Sara; Congdon, Victoria; Cuddy, Meaghan; Hall, Wayne; Becker, Madison; Meiman, Joe; Whiteaker, Tim; Bohannon, Patrick; Grubbs, Faye; Hobson, Cindy; The University of Texas at Austin (2018). Seagrass canopy height, water depth, chlorophyll-a concentration and other plant and water quality indicators in Coastal Waters of Texas (NCEI Accession 0181898). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://accession.nodc.noaa.gov/0181898. Accessed [date].
Cited Authors
  • Dunton, Ken
  • Jackson, Kim
  • Wilson, Sara
  • Congdon, Victoria
  • Cuddy, Meaghan
  • Hall, Wayne
  • Becker, Madison
  • Meiman, Joe
  • Whiteaker, Tim
  • Bohannon, Patrick
  • Grubbs, Faye
  • Hobson, Cindy
  • The University of Texas at Austin
Principal Investigators
Collaborators
  • Kim Jackson
    University of Texas; University of Texas - Austin; Marine Science Institute (UT - Austin Marine Science Institute)
  • Sara Wilson
    University of Texas; University of Texas - Austin; Marine Science Institute (UT - Austin Marine Science Institute)
  • Victoria Congdon
    University of Texas; University of Texas - Austin; Marine Science Institute (UT - Austin Marine Science Institute)
  • Wayne Hall
    University of Texas; University of Texas - Austin; Marine Science Institute (UT - Austin Marine Science Institute)
  • Madison Becker
    University of Texas; University of Texas - Austin; Marine Science Institute (UT - Austin Marine Science Institute)
  • Meiman Joe
    US DOI; National Park Service (NPS)
  • Meaghan Cuddy
    University of Texas; University of Texas - Austin; Marine Science Institute (UT - Austin Marine Science Institute)
  • Patrick Bohannon
    Texas Commission on Environmental Quality
  • Faye Grubbs
    Texas Parks and Wildlife Department
  • Cindy Hobson
    Texas Parks and Wildlife Department
Contributors
Resource Providers
Points of Contact
Publishers
Acknowledgments
  • Related Funding Agency: Mission-Aransas National Estuarine Research Reserve
  • Related Funding Agency: Coastal Bend Bays & Estuaries Program
  • Related Funding Agency: Texas General Land Office
  • Related Funding Agency: Texas Coastal Management Program
  • Related Funding Agency: US DOC; National Oceanic and Atmospheric Administration
  • Related Funding Agency: US DOI; National Park Service
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
Global Change Master Directory (GCMD) Science Keywords
  • EARTH SCIENCE > OCEANS > BATHYMETRY/SEAFLOOR TOPOGRAPHY > WATER DEPTH
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CHLOROPHYLL
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > NITROGEN
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > OXYGEN
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > SUSPENDED SOLIDS
  • EARTH SCIENCE > OCEANS > OCEAN OPTICS > ATTENUATION/TRANSMISSION
  • EARTH SCIENCE > OCEANS > OCEAN OPTICS > SECCHI DEPTH
  • EARTH SCIENCE > OCEANS > OCEAN TEMPERATURE > WATER TEMPERATURE
  • EARTH SCIENCE > OCEANS > SALINITY/DENSITY > SALINITY
Provider Keywords
  • Carbon to Nitrogen Ratio
  • Carbon to Phosphorus Ratio
  • Nitrogen to Phosphorus Ratio
  • Sea Grasses - Canopy Height
  • Sea Grasses - Percent Cover
Data Center keywords Global Change Master Directory (GCMD) Data Center Keywords
  • DOC/NOAA/NESDIS/NODC > National Oceanographic Data Center, NESDIS, NOAA, U.S. Department of Commerce
  • DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
  • DOI/NPS > National Park Service, U.S. Department of the Interior
NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS
Instrument keywords NODC INSTRUMENT TYPES THESAURUS Global Change Master Directory (GCMD) Instrument Keywords
  • MASS SPECTROMETERS
  • SECCHI DISKS
  • VISUAL OBSERVATIONS
Provider Instrument Types
  • PVC marked in 10 cm segments
Place keywords NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords
  • OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > GULF OF MEXICO
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Dunton, Ken; Jackson, Kim; Wilson, Sara; Congdon, Victoria; Cuddy, Meaghan; Hall, Wayne; Becker, Madison; Meiman, Joe; Whiteaker, Tim; Bohannon, Patrick; Grubbs, Faye; Hobson, Cindy; The University of Texas at Austin (2018). Seagrass canopy height, water depth, chlorophyll-a concentration and other plant and water quality indicators in Coastal Waters of Texas (NCEI Accession 0181898). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://accession.nodc.noaa.gov/0181898. Accessed [date].
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees
  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2018-12-19T21:10:51Z - NCEI Accession 0181898 v1.1 was published.
Output Datasets
Lineage information for: dataset
Processing Steps
  • Parameter or Variable: CHLOROPHYLL A CONCENTRATION (measured); Units: microgram/liter; Observation Category: in situ; Sampling Instrument: YSI - handheld multi-parameter instrument; Sampling and Analyzing Method: Stations are approached slowly to ensure that the deceleration of the boat does not create a wake and resuspend sediments into the water column. All hydrographic and water column measurements are collected while the vessel is set to drift over the station to minimize interference from sediment plumes generated from physical disturbances of the bottom. Sonde deployment, water transparency and water sample collection must occur within 30 m of the station location. Measurements are made in the following order: (1) PAR, (2) visibility, (3) hydrography, (4) TSS, (5) depth, (6) percent coverage, (7) canopy height, and (8) blade samples for C:N:P ratios. While taking measurements, care is taken not to disturb the sediment or agitate seagrasses. The sonde is lowered so that the instrument probes are completely submerged.; Data Quality Method: Data are checked against reasonable value ranges. A value of 9999 indicates no data available..
  • Parameter or Variable: water depth (measured); Units: meter; Observation Category: in situ; Sampling Instrument: PVC marked in 10 cm segments; Sampling and Analyzing Method: Stations are approached slowly to ensure that the deceleration of the boat does not create a wake and resuspend sediments into the water column. All hydrographic and water column measurements are collected while the vessel is set to drift over the station to minimize interference from sediment plumes generated from physical disturbances of the bottom. Sonde deployment, water transparency and water sample collection must occur within 30 m of the station location. Measurements are made in the following order: (1) PAR, (2) visibility, (3) hydrography, (4) TSS, (5) depth, (6) percent coverage, (7) canopy height, and (8) blade samples for C:N:P ratios. While taking measurements, care is taken not to disturb the sediment or agitate seagrasses. Depth is measured using a PVC pipe marked in 10 cm segments. Care is taken to avoid allowing the PVC pole to sink into soft sediments.; Data Quality Method: Data are checked against reasonable value ranges. A value of 9999 indicates no data available..
  • Parameter or Variable: DISSOLVED OXYGEN (measured); Units: milligram/liter; Observation Category: in situ; Sampling Instrument: YSI - handheld multi-parameter instrument; Sampling and Analyzing Method: Stations are approached slowly to ensure that the deceleration of the boat does not create a wake and resuspend sediments into the water column. All hydrographic and water column measurements are collected while the vessel is set to drift over the station to minimize interference from sediment plumes generated from physical disturbances of the bottom. Sonde deployment, water transparency and water sample collection must occur within 30 m of the station location. Measurements are made in the following order: (1) PAR, (2) visibility, (3) hydrography, (4) TSS, (5) depth, (6) percent coverage, (7) canopy height, and (8) blade samples for C:N:P ratios. While taking measurements, care is taken not to disturb the sediment or agitate seagrasses. The sonde is lowered so that the instrument probes are completely submerged.; Data Quality Method: Data are checked against reasonable value ranges. A value of 9999 indicates no data available..
  • Parameter or Variable: LIGHT ATTENUATION (measured); Units: kd/meter; Observation Category: in situ; Sampling Instrument: PAR Sensor; Sampling and Analyzing Method: Measurements of percent surface irradiance (% SI) and the diffuse light attenuation coefficient (k) are made from simultaneous measurements of surface (ambient) and underwater irradiance. Surface measurements of photosynthetically active radiation (PAR = ca. 400 to 700 nm wavelength) are collected using an LI-190SA quantum sensor that provides input to a LI-1000 datalogger (LI-COR Inc., Lincoln, Nebraska, USA). Underwater measurements are made using a LI-192SA or LI-193SA spherical quantum sensor. Measurements of % SI and k are based on three or more replicate determinations of instantaneous PAR collected by the surface and underwater sensors and recorded by the LI-1000 datalogger. Care is taken to reduce extraneous sources of reflected light (from boats or clothing). Percent surface irradiance available at the seagrass canopy is calculated as follows: % SI = (Iz/I0) x 100 where Iz and I0 are irradiance (μmol photons m-2sec-1) at depth z (meters) and at the surface, respectively. Light attenuation is calculated using the transformed Beer-Lambert equation: kd = -[ln(Iz/I0)]/z where k is the attenuation coefficient (m-1) and Iz and I0 are irradiance (μmol photons m-2 sec-1) at depth z (meters) and at the surface, respectively.; Data Quality Method: Data are checked against reasonable value ranges. A value of 9999 indicates no data available..
  • Parameter or Variable: pH (measured); Units: pH; Observation Category: in situ; Sampling Instrument: YSI - handheld multi-parameter instrument; Sampling and Analyzing Method: Stations are approached slowly to ensure that the deceleration of the boat does not create a wake and resuspend sediments into the water column. All hydrographic and water column measurements are collected while the vessel is set to drift over the station to minimize interference from sediment plumes generated from physical disturbances of the bottom. Sonde deployment, water transparency and water sample collection must occur within 30 m of the station location. Measurements are made in the following order: (1) PAR, (2) visibility, (3) hydrography, (4) TSS, (5) depth, (6) percent coverage, (7) canopy height, and (8) blade samples for C:N:P ratios. While taking measurements, care is taken not to disturb the sediment or agitate seagrasses. The sonde is lowered so that the instrument probes are completely submerged.; Data Quality Method: Data are checked against reasonable value ranges. A value of 9999 indicates no data available..
  • Parameter or Variable: SEA GRASSES - PERCENT COVER (measured); Units: percent; Observation Category: in situ; Sampling Instrument: visual observation; Sampling and Analyzing Method: Species composition and areal coverage were obtained from four replicate quadrat samples per station at each of the four cardinal locations from the vessel. Percent cover of areal biomass was estimated by direct observation, looking down at the seagrass canopy through the water using a 0.25 m2 quadrat framer subdivided into 100 cells.; Data Quality Method: Data are checked against reasonable value ranges (0 to 100). A value of 9999 indicates no data available..
  • Parameter or Variable: SALINITY (measured); Units: ppt; Observation Category: in situ; Sampling Instrument: YSI - handheld multi-parameter instrument; Sampling and Analyzing Method: Stations are approached slowly to ensure that the deceleration of the boat does not create a wake and resuspend sediments into the water column. All hydrographic and water column measurements are collected while the vessel is set to drift over the station to minimize interference from sediment plumes generated from physical disturbances of the bottom. Sonde deployment, water transparency and water sample collection must occur within 30 m of the station location. Measurements are made in the following order: (1) PAR, (2) visibility, (3) hydrography, (4) TSS, (5) depth, (6) percent coverage, (7) canopy height, and (8) blade samples for C:N:P ratios. While taking measurements, care is taken not to disturb the sediment or agitate seagrasses. The sonde is lowered so that the instrument probes are completely submerged.; Data Quality Method: Data are checked against reasonable value ranges. A value of 9999 indicates no data available..
  • Parameter or Variable: SEA GRASSES - CANOPY HEIGHT (measured); Units: centimeter; Observation Category: in situ; Sampling Instrument: visual observation; Sampling and Analyzing Method: The four corners of the boat (port-stern, port-bow, starboard-bow, and starboard-stern) are used as seagrass sample locations. Measurements are taken approximately 2-4 meters from the boat by tossing a 0.25 m2 quadrat into the water. Within each quadrat, five canopy height measurements are made for seagrass species present.; Data Quality Method: Data are checked against reasonable value ranges. A value of 9999 indicates no data available..
  • Parameter or Variable: SECCHI DEPTH (measured); Units: meter; Observation Category: in situ; Sampling Instrument: secchi disk; Sampling and Analyzing Method: Stations are approached slowly to ensure that the deceleration of the boat does not create a wake and resuspend sediments into the water column. All hydrographic and water column measurements are collected while the vessel is set to drift over the station to minimize interference from sediment plumes generated from physical disturbances of the bottom. Sonde deployment, water transparency and water sample collection must occur within 30 m of the station location. Measurements are made in the following order: (1) PAR, (2) visibility, (3) hydrography, (4) TSS, (5) depth, (6) percent coverage, (7) canopy height, and (8) blade samples for C:N:P ratios. While taking measurements, care is taken not to disturb the sediment or agitate seagrasses. When using a secchi disk for visibility measurements, sunglasses are removed and care is taken to prevent the vessel from shading the disk.; Data Quality Method: Data are checked against reasonable value ranges. A value of 9999 indicates no data available..
  • Parameter or Variable: WATER TEMPERATURE (measured); Units: degrees Celsius; Observation Category: in situ; Sampling Instrument: YSI - handheld multi-parameter instrument; Sampling and Analyzing Method: Stations are approached slowly to ensure that the deceleration of the boat does not create a wake and resuspend sediments into the water column. All hydrographic and water column measurements are collected while the vessel is set to drift over the station to minimize interference from sediment plumes generated from physical disturbances of the bottom. Sonde deployment, water transparency and water sample collection must occur within 30 m of the station location. Measurements are made in the following order: (1) PAR, (2) visibility, (3) hydrography, (4) TSS, (5) depth, (6) percent coverage, (7) canopy height, and (8) blade samples for C:N:P ratios. While taking measurements, care is taken not to disturb the sediment or agitate seagrasses. The sonde is lowered so that the instrument probes are completely submerged.; Data Quality Method: Data are checked against reasonable value ranges. A value of 9999 indicates no data available..
  • Parameter or Variable: suspended solids (measured); Units: milligram/liter; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Replicate water samples were obtained at each station for determination of Total Suspended Solid (TSS) concentration. Bottles and lids are rinsed three times with sample water before collecting the sample. Gloves are worn if the collector is wearing sunscreen to prevent contamination of sample. The TSS method is adapted from EPA Method 160.2. Summary of Method: A well-mixed sample is filtered through a glass fiber filter, and the residue retained on the filter is dried to constant weight at 103-105°C. Sample Handling and Preservation Non-representative particulates such as leaves, sticks, fish, and lumps of fecal matter should be excluded from the sample if it is determined that their inclusion is not desired in the final result. Preservation of the sample is not practical; analysis should begin as soon as possible. Refrigeration or icing to 4°C, to minimize microbiological decomposition of solids, is recommended. Interferences: Filtration apparatus, filter material, pre-washing, post-washing, and drying temperature are specified because these variables have been shown to affect the results. Samples high in Filterable Residue (dissolved solids), such as saline waters, brines and some wastes, may be subject to a positive interference. Care must be taken in selecting the filtering apparatus so that washing of the filter and any dissolved solids in the filter minimizes this potential interference. Laboratory Procedures: 1) Dry new filters at 60°C in oven prior to use. 2) Weigh filter immediately before use. After weighing, handle the filter or crucible/filter with forceps or tongs only. 3) Place the glass fiber filter (i.e. Glass fiber filter discs, without organic binder, such as Millipore AP-40, Reeves Angel 934-AH, Gelman type A/E, or equivalent; Our lab uses 47 mm GF/F 0.7 micron retention) on the membrane filter apparatus. NOTE: Because of the physical nature of glass fiber filters, the absolute pore size cannot be controlled or measured. Terms such as “pore size”, “collection efficiencies” and “effective retention” are used to define this property in glass fiber filters. 4) For a 47 mm diameter filter, filter 100 mL of sample. If weight of captured residue is less than 1.0 mg, the sample volume must be increased to provide at least 1.0 mg of residue. If other filter diameters are used, start with a sample volume equal to 7 mL/cm of filter area and collect at least a weight of residue proportional to the 1.0 mg state above. NOTE: If filtering clear pristine water, start with 1 L. If filtering turbid water start with 100 mL. NOTE: If during filtration of this initial volume the filtration rate drops rapidly, or if filtration time exceeds 5 to 10 minutes, the following scheme is recommended: Use an unweighed glass fiber filter affixed in the filter assembly. Add a known volume of sample to the filter funnel and record the time elapsed after selected volumes have passed through the filter. Twenty-five mL increments for timing are suggested. Continue to record the time and volume increments until filtration rate drops rapidly. Add additional sample if the filter funnel volume is inadequate to reach a reduced rate. Plot the observed time versus volume filtered. Select the proper filtration volume as that just short of the time a significant change in filtration rate occurred. 5) Assemble the filtering apparatus and begin suction. 6) Shake the sample vigorously and quantitatively transfer the predetermined sample volume selected to the filter using a graduated cylinder. Pour into funnel. 7) Remove all traces of water by continuing to apply vacuum after sample has passed through. 8) With suction on, wash the graduated cylinder, filter, non-filterable residue and filter funnel wall with three portions of distilled water allowing complete drainage between washing. Remove all traces of water by continuing to apply vacuum after water has passed through. NOTE: Total volume of distilled rinse water used should equal no less than 50 mLs following complete filtration of sample volume. 9) Carefully remove the filter from the filter support. 10) Dry at least one hour at 103-105°C. Overnight drying ensures accurate filter weight. 11) Cool in a desiccator and weigh. 12) Repeat the drying cycle until a constant weight is obtained (weight loss is less than 0.5 mg). Calculations: TSS (mg/L) is calculated as follows: 1000 x (A-B) x (1000/C) = TSS where A = weight of filter (or filter and crucible) + residue (mg), B = weight of filter (or filter and crucible) (mg), and C = amount of sample filtered (mL).; Data Quality Method: Data are checked against reasonable value ranges. A value of 9999 indicates no data available..
  • Parameter or Variable: Carbon content of specimen (calculated); Units: percent; Observation Category: laboratory analysis; Sampling Instrument: mass spectrometer; Sampling and Analyzing Method: Collect samples of Thalassia and Halodule blades by manually snapping the seagrass shoot from the rhizome and keeping the sheath intact. Rinse sediment from tissue samples in the field. Be careful not to contaminate samples with sunscreen, lotion, etc. Roll the seagrass blades (minimum of 6 for Thalassia and 15 for Halodule) and place in a labeled Whirl-pak bag, removing all air, sealing, and placing on ice. In the lab, newly formed leaves (the youngest leaf in a shoot bundle) are gently scraped and rinsed in tap water to remove algal and faunal epiphytes. The rinsed tissue samples are then dried to a constant weight at 60°C and homogenized by grinding to a fine powder using a mortar and pestle. Tissue C:N Content, del-C13 and del-N15 tissue samples are analyzed for carbon and nitrogen concentrations and isotopic values using either a PDZ Europa ANCA-GSL elemental analyzer coupled to a PDZ Europa 20-20 isotope ratio mass spectrometer (UC-Davis; precision 0.2 per mille for 13C and 0.3 per mille for 15N), or a Carlo Erba 2500 elemental analyzer coupled to a Finnigan MAT DELTAplus isotope ratio mass spectrometer 23 (UTMSI; precision 0.3 per mille for both 13C and 15N).; Data Quality Method: The EA-IRMS (all C and N parameters) is calibrated and run against known reference standards from the National Institute of Standards and Technology (NIST) to ensure that machine is running properly. Our CORE lab technician also performs regular independent checks and calibrations. This is done every every 10 samples, along with a size series at the set of samples. Blank values indicate the analysis was not run..
  • Parameter or Variable: Nitrogen content of specimen (measured); Units: percent; Observation Category: laboratory analysis; Sampling Instrument: mass spectrometer; Sampling and Analyzing Method: Collect samples of Thalassia and Halodule blades by manually snapping the seagrass shoot from the rhizome and keeping the sheath intact. Rinse sediment from tissue samples in the field. Be careful not to contaminate samples with sunscreen, lotion, etc. Roll the seagrass blades (minimum of 6 for Thalassia and 15 for Halodule) and place in a labeled Whirl-pak bag, removing all air, sealing, and placing on ice. In the lab, newly formed leaves (the youngest leaf in a shoot bundle) are gently scraped and rinsed in tap water to remove algal and faunal epiphytes. The rinsed tissue samples are then dried to a constant weight at 60°C and homogenized by grinding to a fine powder using a mortar and pestle. Tissue C:N Content, del-C13 and del-N15 tissue samples are analyzed for carbon and nitrogen concentrations and isotopic values using either a PDZ Europa ANCA-GSL elemental analyzer coupled to a PDZ Europa 20-20 isotope ratio mass spectrometer (UC-Davis; precision 0.2 per mille for 13C and 0.3 per mille for 15N), or a Carlo Erba 2500 elemental analyzer coupled to a Finnigan MAT DELTAplus isotope ratio mass spectrometer 23 (UTMSI; precision 0.3 per mille for both 13C and 15N).; Data Quality Method: The EA-IRMS (all C and N parameters) is calibrated and run against known reference standards from the National Institute of Standards and Technology (NIST) to ensure that machine is running properly. Our CORE lab technician also performs regular independent checks and calibrations. This is done every every 10 samples, along with a size series at the set of samples. Blank values indicate the analysis was not run..
  • Parameter or Variable: TOTAL PHOSPHORUS (calculated); Units: percent; Observation Category: laboratory analysis; Sampling Instrument: laboratory analysis; Sampling and Analyzing Method: Collect samples of Thalassia and Halodule blades by manually snapping the seagrass shoot from the rhizome and keeping the sheath intact. Rinse sediment from tissue samples in the field. Be careful not to contaminate samples with sunscreen, lotion, etc. Roll the seagrass blades (minimum of 6 for Thalassia and 15 for Halodule) and place in a labeled Whirl-pak bag, removing all air, sealing, and placing on ice. In the lab, newly formed leaves (the youngest leaf in a shoot bundle) are gently scraped and rinsed in tap water to remove algal and faunal epiphytes. The rinsed tissue samples are then dried to a constant weight at 60°C and homogenized by grinding to a fine powder using a mortar and pestle. Tissue phosphorous content is determined following procedures described in [Chapman, H.D. and Pratt, P.F. 1961. Methods of Analysis for Soils, Plants and Water. Univ. California, Berkeley, CA, USA].; Data Quality Method: P analysis is run against a prepared standard curve approximately every 20 samples to ensure the spec is properly calibrated and reading correctly. If a value is extremely abnormal (for example, an order of magnitude higher or lower than what would be expected, clearly indicating some sort of contamination) it is excluded from the dataset. A value of -9991 indicates not enough phosphorus sample to run. Blank values indicate the analysis was not run..
  • Parameter or Variable: Carbon content of specimen (measured); Units: micromole/milligram; Observation Category: laboratory analysis; Sampling Instrument: mass spectrometer; Sampling and Analyzing Method: Collect samples of Thalassia and Halodule blades by manually snapping the seagrass shoot from the rhizome and keeping the sheath intact. Rinse sediment from tissue samples in the field. Be careful not to contaminate samples with sunscreen, lotion, etc. Roll the seagrass blades (minimum of 6 for Thalassia and 15 for Halodule) and place in a labeled Whirl-pak bag, removing all air, sealing, and placing on ice. In the lab, newly formed leaves (the youngest leaf in a shoot bundle) are gently scraped and rinsed in tap water to remove algal and faunal epiphytes. The rinsed tissue samples are then dried to a constant weight at 60°C and homogenized by grinding to a fine powder using a mortar and pestle. Tissue C:N Content, del-C13 and del-N15 tissue samples are analyzed for carbon and nitrogen concentrations and isotopic values using either a PDZ Europa ANCA-GSL elemental analyzer coupled to a PDZ Europa 20-20 isotope ratio mass spectrometer (UC-Davis; precision 0.2 per mille for 13C and 0.3 per mille for 15N), or a Carlo Erba 2500 elemental analyzer coupled to a Finnigan MAT DELTAplus isotope ratio mass spectrometer 23 (UTMSI; precision 0.3 per mille for both 13C and 15N).; Data Quality Method: The EA-IRMS (all C and N parameters) is calibrated and run against known reference standards from the National Institute of Standards and Technology (NIST) to ensure that machine is running properly. Our CORE lab technician also performs regular independent checks and calibrations. This is done every every 10 samples, along with a size series at the set of samples. Blank values indicate the analysis was not run..
  • Parameter or Variable: Nitrogen content of specimen (measured); Units: micromole/milligram; Observation Category: laboratory analysis; Sampling Instrument: mass spectrometer; Sampling and Analyzing Method: Collect samples of Thalassia and Halodule blades by manually snapping the seagrass shoot from the rhizome and keeping the sheath intact. Rinse sediment from tissue samples in the field. Be careful not to contaminate samples with sunscreen, lotion, etc. Roll the seagrass blades (minimum of 6 for Thalassia and 15 for Halodule) and place in a labeled Whirl-pak bag, removing all air, sealing, and placing on ice. In the lab, newly formed leaves (the youngest leaf in a shoot bundle) are gently scraped and rinsed in tap water to remove algal and faunal epiphytes. The rinsed tissue samples are then dried to a constant weight at 60°C and homogenized by grinding to a fine powder using a mortar and pestle. Tissue C:N Content, del-C13 and del-N15 tissue samples are analyzed for carbon and nitrogen concentrations and isotopic values using either a PDZ Europa ANCA-GSL elemental analyzer coupled to a PDZ Europa 20-20 isotope ratio mass spectrometer (UC-Davis; precision 0.2 per mille for 13C and 0.3 per mille for 15N), or a Carlo Erba 2500 elemental analyzer coupled to a Finnigan MAT DELTAplus isotope ratio mass spectrometer 23 (UTMSI; precision 0.3 per mille for both 13C and 15N).; Data Quality Method: The EA-IRMS (all C and N parameters) is calibrated and run against known reference standards from the National Institute of Standards and Technology (NIST) to ensure that machine is running properly. Our CORE lab technician also performs regular independent checks and calibrations. This is done every every 10 samples, along with a size series at the set of samples. Blank values indicate the analysis was not run..
  • Parameter or Variable: TOTAL PHOSPHORUS (calculated); Units: micromole/milligram; Observation Category: laboratory analysis; Sampling Instrument: laboratory analysis; Sampling and Analyzing Method: Collect samples of Thalassia and Halodule blades by manually snapping the seagrass shoot from the rhizome and keeping the sheath intact. Rinse sediment from tissue samples in the field. Be careful not to contaminate samples with sunscreen, lotion, etc. Roll the seagrass blades (minimum of 6 for Thalassia and 15 for Halodule) and place in a labeled Whirl-pak bag, removing all air, sealing, and placing on ice. In the lab, newly formed leaves (the youngest leaf in a shoot bundle) are gently scraped and rinsed in tap water to remove algal and faunal epiphytes. The rinsed tissue samples are then dried to a constant weight at 60°C and homogenized by grinding to a fine powder using a mortar and pestle. Tissue phosphorous content is determined following procedures described in [Chapman, H.D. and Pratt, P.F. 1961. Methods of Analysis for Soils, Plants and Water. Univ. California, Berkeley, CA, USA].; Data Quality Method: P analysis is run against a prepared standard curve approximately every 20 samples to ensure the spec is properly calibrated and reading correctly. If a value is extremely abnormal (for example, an order of magnitude higher or lower than what would be expected, clearly indicating some sort of contamination) it is excluded from the dataset. A value of -9991 indicates not enough phosphorus sample to run. Blank values indicate the analysis was not run..
  • Parameter or Variable: DELTA CARBON-13 (measured); Units: per mille; Observation Category: laboratory analysis; Sampling Instrument: mass spectrometer; Sampling and Analyzing Method: Collect samples of Thalassia and Halodule blades by manually snapping the seagrass shoot from the rhizome and keeping the sheath intact. Rinse sediment from tissue samples in the field. Be careful not to contaminate samples with sunscreen, lotion, etc. Roll the seagrass blades (minimum of 6 for Thalassia and 15 for Halodule) and place in a labeled Whirl-pak bag, removing all air, sealing, and placing on ice. In the lab, newly formed leaves (the youngest leaf in a shoot bundle) are gently scraped and rinsed in tap water to remove algal and faunal epiphytes. The rinsed tissue samples are then dried to a constant weight at 60°C and homogenized by grinding to a fine powder using a mortar and pestle. Tissue C:N Content, del-C13 and del-N15 tissue samples are analyzed for carbon and nitrogen concentrations and isotopic values using either a PDZ Europa ANCA-GSL elemental analyzer coupled to a PDZ Europa 20-20 isotope ratio mass spectrometer (UC-Davis; precision 0.2 per mille for 13C and 0.3 per mille for 15N), or a Carlo Erba 2500 elemental analyzer coupled to a Finnigan MAT DELTAplus isotope ratio mass spectrometer 23 (UTMSI; precision 0.3 per mille for both 13C and 15N).; Data Quality Method: The EA-IRMS (all C and N parameters) is calibrated and run against known reference standards from the National Institute of Standards and Technology (NIST) to ensure that machine is running properly. Our CORE lab technician also performs regular independent checks and calibrations. This is done every every 10 samples, along with a size series at the set of samples. Blank values indicate the analysis was not run..
  • Parameter or Variable: DELTA NITROGEN-15 (measured); Units: per mille; Observation Category: laboratory analysis; Sampling Instrument: mass spectrometer; Sampling and Analyzing Method: Collect samples of Thalassia and Halodule blades by manually snapping the seagrass shoot from the rhizome and keeping the sheath intact. Rinse sediment from tissue samples in the field. Be careful not to contaminate samples with sunscreen, lotion, etc. Roll the seagrass blades (minimum of 6 for Thalassia and 15 for Halodule) and place in a labeled Whirl-pak bag, removing all air, sealing, and placing on ice. In the lab, newly formed leaves (the youngest leaf in a shoot bundle) are gently scraped and rinsed in tap water to remove algal and faunal epiphytes. The rinsed tissue samples are then dried to a constant weight at 60°C and homogenized by grinding to a fine powder using a mortar and pestle. Tissue C:N Content, del-C13 and del-N15 tissue samples are analyzed for carbon and nitrogen concentrations and isotopic values using either a PDZ Europa ANCA-GSL elemental analyzer coupled to a PDZ Europa 20-20 isotope ratio mass spectrometer (UC-Davis; precision 0.2 per mille for 13C and 0.3 per mille for 15N), or a Carlo Erba 2500 elemental analyzer coupled to a Finnigan MAT DELTAplus isotope ratio mass spectrometer 23 (UTMSI; precision 0.3 per mille for both 13C and 15N).; Data Quality Method: The EA-IRMS (all C and N parameters) is calibrated and run against known reference standards from the National Institute of Standards and Technology (NIST) to ensure that machine is running properly. Our CORE lab technician also performs regular independent checks and calibrations. This is done every every 10 samples, along with a size series at the set of samples. Blank values indicate the analysis was not run..
  • Parameter or Variable: Carbon to Nitrogen Ratio (calculated); Units: dimensionless; Observation Category: laboratory analysis; Sampling Instrument: laboratory analysis; Sampling and Analyzing Method: Collect samples of Thalassia and Halodule blades by manually snapping the seagrass shoot from the rhizome and keeping the sheath intact. Rinse sediment from tissue samples in the field. Be careful not to contaminate samples with sunscreen, lotion, etc. Roll the seagrass blades (minimum of 6 for Thalassia and 15 for Halodule) and place in a labeled Whirl-pak bag, removing all air, sealing, and placing on ice. In the lab, newly formed leaves (the youngest leaf in a shoot bundle) are gently scraped and rinsed in tap water to remove algal and faunal epiphytes. The rinsed tissue samples are then dried to a constant weight at 60°C and homogenized by grinding to a fine powder using a mortar and pestle. Tissue C:N Content, del-C13 and del-N15 tissue samples are analyzed for carbon and nitrogen concentrations and isotopic values using either a PDZ Europa ANCA-GSL elemental analyzer coupled to a PDZ Europa 20-20 isotope ratio mass spectrometer (UC-Davis; precision 0.2 per mille for 13C and 0.3 per mille for 15N), or a Carlo Erba 2500 elemental analyzer coupled to a Finnigan MAT DELTAplus isotope ratio mass spectrometer 23 (UTMSI; precision 0.3 per mille for both 13C and 15N).; Data Quality Method: The EA-IRMS (all C and N parameters) is calibrated and run against known reference standards from the National Institute of Standards and Technology (NIST) to ensure that machine is running properly. Our CORE lab technician also performs regular independent checks and calibrations. This is done every every 10 samples, along with a size series at the set of samples. Blank values indicate the analysis was not run..
  • Parameter or Variable: Carbon to Phosphorus Ratio (calculated); Units: dimensionless; Observation Category: laboratory analysis; Sampling Instrument: laboratory analysis; Sampling and Analyzing Method: Collect samples of Thalassia and Halodule blades by manually snapping the seagrass shoot from the rhizome and keeping the sheath intact. Rinse sediment from tissue samples in the field. Be careful not to contaminate samples with sunscreen, lotion, etc. Roll the seagrass blades (minimum of 6 for Thalassia and 15 for Halodule) and place in a labeled Whirl-pak bag, removing all air, sealing, and placing on ice. In the lab, newly formed leaves (the youngest leaf in a shoot bundle) are gently scraped and rinsed in tap water to remove algal and faunal epiphytes. The rinsed tissue samples are then dried to a constant weight at 60°C and homogenized by grinding to a fine powder using a mortar and pestle. Tissue C:N Content, del-C13 and del-N15 tissue samples are analyzed for carbon and nitrogen concentrations and isotopic values using either a PDZ Europa ANCA-GSL elemental analyzer coupled to a PDZ Europa 20-20 isotope ratio mass spectrometer (UC-Davis; precision 0.2 per mille for 13C and 0.3 per mille for 15N), or a Carlo Erba 2500 elemental analyzer coupled to a Finnigan MAT DELTAplus isotope ratio mass spectrometer 23 (UTMSI; precision 0.3 per mille for both 13C and 15N). Tissue phosphorous content is determined following procedures described in [Chapman, H.D. and Pratt, P.F. 1961. Methods of Analysis for Soils, Plants and Water. Univ. California, Berkeley, CA, USA].; Data Quality Method: The EA-IRMS (all C and N parameters) is calibrated and run against known reference standards from the National Institute of Standards and Technology (NIST) to ensure that machine is running properly. Our CORE lab technician also performs regular independent checks and calibrations. This is done every every 10 samples, along with a size series at the set of samples. P analysis is run against a prepared standard curve approximately every 20 samples to ensure the spec is properly calibrated and reading correctly. If a value is extremely abnormal (for example, an order of magnitude higher or lower than what would be expected, clearly indicating some sort of contamination) it is excluded from the dataset. A value of -9991 indicates not enough phosphorus sample to run. Blank values indicate the analysis was not run..
  • Parameter or Variable: Nitrogen to Phosphorus Ratio (calculated); Units: dimensionless; Observation Category: laboratory analysis; Sampling Instrument: laboratory analysis; Sampling and Analyzing Method: Collect samples of Thalassia and Halodule blades by manually snapping the seagrass shoot from the rhizome and keeping the sheath intact. Rinse sediment from tissue samples in the field. Be careful not to contaminate samples with sunscreen, lotion, etc. Roll the seagrass blades (minimum of 6 for Thalassia and 15 for Halodule) and place in a labeled Whirl-pak bag, removing all air, sealing, and placing on ice. In the lab, newly formed leaves (the youngest leaf in a shoot bundle) are gently scraped and rinsed in tap water to remove algal and faunal epiphytes. The rinsed tissue samples are then dried to a constant weight at 60°C and homogenized by grinding to a fine powder using a mortar and pestle. Tissue C:N Content, del-C13 and del-N15 tissue samples are analyzed for carbon and nitrogen concentrations and isotopic values using either a PDZ Europa ANCA-GSL elemental analyzer coupled to a PDZ Europa 20-20 isotope ratio mass spectrometer (UC-Davis; precision 0.2 per mille for 13C and 0.3 per mille for 15N), or a Carlo Erba 2500 elemental analyzer coupled to a Finnigan MAT DELTAplus isotope ratio mass spectrometer 23 (UTMSI; precision 0.3 per mille for both 13C and 15N). Tissue phosphorous content is determined following procedures described in [Chapman, H.D. and Pratt, P.F. 1961. Methods of Analysis for Soils, Plants and Water. Univ. California, Berkeley, CA, USA].; Data Quality Method: The EA-IRMS (all C and N parameters) is calibrated and run against known reference standards from the National Institute of Standards and Technology (NIST) to ensure that machine is running properly. Our CORE lab technician also performs regular independent checks and calibrations. This is done every every 10 samples, along with a size series at the set of samples. P analysis is run against a prepared standard curve approximately every 20 samples to ensure the spec is properly calibrated and reading correctly. If a value is extremely abnormal (for example, an order of magnitude higher or lower than what would be expected, clearly indicating some sort of contamination) it is excluded from the dataset. A value of -9991 indicates not enough phosphorus sample to run. Blank values indicate the analysis was not run..
  • Parameter or Variable: CHLOROPHYLL A CONCENTRATION (measured); Units: microgram/liter; Observation Category: laboratory analysis; Sampling Instrument: bottle; Sampling and Analyzing Method: Analyzed following EPA 445.0*; Chlorophyll a - fluorometic method, with a modification contained in TCEQ SOP - HLAB 158; Data Quality Method: TCEQ SOP - HLAB 158.
Acquisition Information (collection)
Instrument
  • bottle
  • laboratory analysis
  • mass spectrometer
  • PAR Sensor
  • secchi disk
  • visual observation
  • YSI - handheld multi-parameter instrument
Last Modified: 2019-01-08T15:37:53Z
For questions about the information on this page, please email: NODC.DataOfficer@noaa.gov