Processing Steps |
- Parameter or Variable: SALINITY (measured); Units: ppt; Observation Category: in situ; Sampling Instrument: Handheld YSI Pro 2030.
- Parameter or Variable: TEMPERATURE - WATER [WATER TEMPERATURE] (measured); Units: degrees Celsius; Observation Category: in situ; Sampling Instrument: Handheld YSI Pro 2031.
- Parameter or Variable: DISSOLVED OXYGEN (measured); Units: mg/L; Observation Category: in situ; Sampling Instrument: Handheld YSI Pro 2032.
- Parameter or Variable: per cent saturation dissolved oxygen (measured); Units: ppm; Observation Category: in situ; Sampling Instrument: Handheld YSI Pro 2032.
- Parameter or Variable: Specific Conductivity (measured); Units: m s/cm; Observation Category: in situ; Sampling Instrument: Handheld YSI Pro 2034.
- Parameter or Variable: turbidity (measured); Units: ntu; Observation Category: in situ; Sampling Instrument: HACH 2100Q Portable Turbidimeter.
- Parameter or Variable: pH (measured); Units: moles per liter; Observation Category: in situ; Sampling Instrument: Orion Star A121 portable pH meter.
- Parameter or Variable: Enterococcus (measured); Units: MPN/100mL; Observation Category: in situ; Sampling Instrument: Collected with sterile 1L bottle, IDEXX MPN Method.
- Parameter or Variable: Clostridium perfringnes (measured); Units: CFU/100mL; Observation Category: in situ; Sampling Instrument: Collected with sterile 1L bottle, standard membrane filtration techniques.
- Parameter or Variable: Est. %N (algae) (measured); Units: undefined; Observation Category: in situ; Sampling Instrument: scoop; Sampling and Analyzing Method: Collected at the shoreline, stored in ziplocs on ice, ground, and analyzed using a Thermo-Finnigan™ Delta V Advantage isotope ratio mass spectrometer (IRMS) with a Conflo III interface and a Costech™ ECS 4010 Elemental Analyzer.
- Parameter or Variable: δ15N vs Air* (algae) (measured); Units: undefined; Observation Category: in situ; Sampling Instrument: scoop; Sampling and Analyzing Method: Collected at the shoreline, stored in ziplocs on ice, ground, and analyzed using a Thermo-Finnigan™ Delta V Advantage isotope ratio mass spectrometer (IRMS) with a Conflo III interface and a Costech™ ECS 4010 Elemental Analyzer.
- Parameter or Variable: d15NAir (water) (measured); Units: undefined; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1L bottle, filtered, and analyzed on a Thermo-Finnigan™ Delta Plus IRMS.
- Parameter or Variable: d18O (water) (measured); Units: undefined; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1L bottle, filtered, and analyzed on Pulse Technicon™ II autoanalyzer.
- Parameter or Variable: NO2+NO3 (measured); Units: µmol/L; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1L bottle, filtered, and analyzed on Pulse Technicon™ II autoanalyzer.
- Parameter or Variable: PHOSPHATE - PO4-P (INORGANIC) [phosphate] (measured); Units: µmol/L; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1L bottle, filtered, and analyzed on Pulse Technicon™ II autoanalyzer.
- Parameter or Variable: H4SiO4 (measured); Units: µmol/L; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1L bottle, filtered, and analyzed on Pulse Technicon™ II autoanalyzer.
- Parameter or Variable: AMMONIUM (NH4) (measured); Units: µmol/L; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1L bottle, filtered, and analyzed on Pulse Technicon™ II autoanalyzer.
- Parameter or Variable: TDP (measured); Units: µmol/L; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1L bottle, filtered, and analyzed on Pulse Technicon™ II autoanalyzer.
- Parameter or Variable: Total Dissolved Nitrogen (TDN) (measured); Units: µmol/L; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1 L bottle, filtered, and analyzed by high-temperature combustion, followed by chemiluminescent detection of nitric oxide.
- Parameter or Variable: DISSOLVED ORGANIC CARBON (measured); Units: µmol/L; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1L bottle, filtered, and analyzed on Pulse Technicon™ II autoanalyzer.
- Parameter or Variable: Enterococcus_avg_MST (measured); Units: Copies/100mL; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1 L bottled, filtered, and DNA extractions were performed using the MoBio Power Soil Kit; filters were stored frozen in the provided bead beating tubes (catalog number: 12888-100-PBT), thawed at room temp 1-2 hours and homogenized in a ThermoSavantFastPrep® FP120 Cell Disrupter for 45 seconds at maximum speed 6.5 m/sec..
- Parameter or Variable: BacHum_avg_MST (measured); Units: Copies/100mL; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1 L bottled, filtered, and DNA extractions were performed using the MoBio Power Soil Kit; filters were stored frozen in the provided bead beating tubes (catalog number: 12888-100-PBT), thawed at room temp 1-2 hours and homogenized in a ThermoSavantFastPrep® FP120 Cell Disrupter for 45 seconds at maximum speed 6.5 m/sec..
- Parameter or Variable: HF183_avg_MST (measured); Units: Copies/100mL; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1 L bottled, filtered, and DNA extractions were performed using the MoBio Power Soil Kit; filters were stored frozen in the provided bead beating tubes (catalog number: 12888-100-PBT), thawed at room temp 1-2 hours and homogenized in a ThermoSavantFastPrep® FP120 Cell Disrupter for 45 seconds at maximum speed 6.5 m/sec..
- Parameter or Variable: Bacteroidales_avg_MST (measured); Units: Copies/100mL; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1 L bottled, filtered, and DNA extractions were performed using the MoBio Power Soil Kit; filters were stored frozen in the provided bead beating tubes (catalog number: 12888-100-PBT), thawed at room temp 1-2 hours and homogenized in a ThermoSavantFastPrep® FP120 Cell Disrupter for 45 seconds at maximum speed 6.5 m/sec..
- Parameter or Variable: E.coli_avg_MST (measured); Units: Copies/100mL; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1 L bottled, filtered, and DNA extractions were performed using the MoBio Power Soil Kit; filters were stored frozen in the provided bead beating tubes (catalog number: 12888-100-PBT), thawed at room temp 1-2 hours and homogenized in a ThermoSavantFastPrep® FP120 Cell Disrupter for 45 seconds at maximum speed 6.5 m/sec..
- Parameter or Variable: Enterococcus_avg_1+log10 (measured); Units: Copies/100mL; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1 L bottled, filtered, and DNA extractions were performed using the MoBio Power Soil Kit; filters were stored frozen in the provided bead beating tubes (catalog number: 12888-100-PBT), thawed at room temp 1-2 hours and homogenized in a ThermoSavantFastPrep® FP120 Cell Disrupter for 45 seconds at maximum speed 6.5 m/sec..
- Parameter or Variable: BacHum_avg_1+log10 (measured); Units: Copies/100mL; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1 L bottled, filtered, and DNA extractions were performed using the MoBio Power Soil Kit; filters were stored frozen in the provided bead beating tubes (catalog number: 12888-100-PBT), thawed at room temp 1-2 hours and homogenized in a ThermoSavantFastPrep® FP120 Cell Disrupter for 45 seconds at maximum speed 6.5 m/sec..
- Parameter or Variable: HF183_avg_1+log10 (measured); Units: Copies/100mL; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1 L bottled, filtered, and DNA extractions were performed using the MoBio Power Soil Kit; filters were stored frozen in the provided bead beating tubes (catalog number: 12888-100-PBT), thawed at room temp 1-2 hours and homogenized in a ThermoSavantFastPrep® FP120 Cell Disrupter for 45 seconds at maximum speed 6.5 m/sec..
- Parameter or Variable: Bacteroidales_avg_1+log10 (measured); Units: Copies/100mL; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1 L bottled, filtered, and DNA extractions were performed using the MoBio Power Soil Kit; filters were stored frozen in the provided bead beating tubes (catalog number: 12888-100-PBT), thawed at room temp 1-2 hours and homogenized in a ThermoSavantFastPrep® FP120 Cell Disrupter for 45 seconds at maximum speed 6.5 m/sec..
- Parameter or Variable: E.coli_avg_1+log10 (measured); Units: Copies/100mL; Observation Category: in situ; Sampling Instrument: bottle; Sampling and Analyzing Method: Collected in sterile 1 L bottled, filtered, and DNA extractions were performed using the MoBio Power Soil Kit; filters were stored frozen in the provided bead beating tubes (catalog number: 12888-100-PBT), thawed at room temp 1-2 hours and homogenized in a ThermoSavantFastPrep® FP120 Cell Disrupter for 45 seconds at maximum speed 6.5 m/sec.
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