Hypoxia and Hydrogen Sulfide (H2S) in Padilla Bay, WA from 2014-07-09 to 2014-09-30 (NCEI Accession 0156596)

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Hypoxia has the potential to be a stressor to eelgrass as it can lead to tissue anoxia at night. These tissues then must undergo anaerobic metabolism, which is less energetically efficient and can produce toxic byproducts. Hypoxia may also work in synergy with other stressors, such as sediment pore-water sulfide. Hypoxia can facilitate the intrusion of sulfide, a known phytotoxin, into eelgrass tissues. This study examined the interaction between sulfide and hypoxia on the growth and photosynthetic efficiency of Zostera marina. Eelgrass shoots were collected from Padilla Bay, Washington and placed into seawater tanks in 18 oz. cups of sediment with a disk of agar at the bottom to simulate organic enrichment and to stimulate sulfide production. The growth rate and photosynthetic efficiency of the eelgrass shoots were monitored weekly for six weeks. After week three, the water columns of six of the tanks were reduced to hypoxic conditions (<2mg L-1). During week 6, the oxygen concentration was dropped further to near anoxic conditions. After week 6, eelgrass tissue samples were collected for measurement of total sulfur, carbon, and nitrogen. The results indicated that hypoxia had a significant negative effect on Z. marina shoots, which was evidenced by strong reductions in growth rates and photosynthetic efficiencies. These findings indicate that hypoxia in conjunction with sediment organic enrichment harms eelgrass health and enhances the intrusion of sulfide into plant tissues, over a wide range of pore-water sulfide concentrations.
  • Cite as: Ciesielski, Melissa; Shull, David; Yang, Sylvia (2016). Hypoxia and Hydrogen Sulfide (H2S) in Padilla Bay, WA from 2014-07-09 to 2014-09-30 (NCEI Accession 0156596). Version 1.1. NOAA National Centers for Environmental Information. Dataset. [access date]
gov.noaa.nodc:0156596
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Distributor DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
301-713-3277
NCEI.Info@noaa.gov
Dataset Point of Contact Information Services
DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
301-713-3277
NCEI.Info@noaa.gov
Time Period 2014-07-09 to 2014-09-30
Spatial Bounding Box Coordinates
N: 48.5
S: 48.49
E: -122.48
W: -122.49
Spatial Coverage Map
General Documentation
Publication Dates
  • publication: 2016-10-18
Edition 1.1
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Data Update Frequency As needed
Supplemental Information
Submission Package ID: EENGYB
Purpose Basic research
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Ciesielski, Melissa; Shull, David; Yang, Sylvia (2016). Hypoxia and Hydrogen Sulfide (H2S) in Padilla Bay, WA from 2014-07-09 to 2014-09-30 (NCEI Accession 0156596). Version 1.1. NOAA National Centers for Environmental Information. Dataset. [access date]
Cited Authors
  • Ciesielski, Melissa
  • Shull, David
  • Yang, Sylvia
Principal Investigators
  • David Shull
Collaborators
  • Sylvia Yang
  • Melissa Ciesielski
Contributors
Resource Providers
Publishers
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
Global Change Master Directory (GCMD) Science and Services Keywords
  • EARTH SCIENCE > BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PHOTOSYNTHESIS
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > CARBON
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > INORGANIC CARBON
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > NITROGEN
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > OXYGEN
  • EARTH SCIENCE > OCEANS > OCEAN OPTICS > PHOTOSYNTHETICALLY ACTIVE RADIATION
Submitter Data Types
  • Total C:N:S
Data Center keywords Global Change Master Directory (GCMD) Data Center Keywords
  • DOC/NOAA/NESDIS/NODC > National Oceanographic Data Center, NESDIS, NOAA, U.S. Department of Commerce
  • DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS
Instrument keywords NODC INSTRUMENT TYPES THESAURUS Global Change Master Directory (GCMD) Instrument Keywords
  • FLUOROMETERS
  • OXYGEN METERS
Submitter Instrument Keywords
  • Apollo SciTech AS-C3 Dissolved Inorganic Carbon Analyzer
  • Elantech Flash EA 1112 elemental analyzer
  • ion selective electrode
  • ruler
Place keywords NODC SEA AREA NAMES THESAURUS Global Change Master Directory (GCMD) Location Keywords
  • OCEAN > PACIFIC OCEAN > NORTH PACIFIC OCEAN
Submitter Place Keywords
  • Padilla Bay
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Ciesielski, Melissa; Shull, David; Yang, Sylvia (2016). Hypoxia and Hydrogen Sulfide (H2S) in Padilla Bay, WA from 2014-07-09 to 2014-09-30 (NCEI Accession 0156596). Version 1.1. NOAA National Centers for Environmental Information. Dataset. [access date]
Access Constraints
  • NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
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Lineage information for: dataset
Processing Steps
  • 2016-10-18T14:59:11 - NCEI Accession 0156596 v1.1 was published.
Output Datasets
Lineage information for: dataset
Processing Steps
  • Data Type: PHOTOSYNTHETIC ACTIVE RADIATION (PAR) (measured); Units: micromole/second/m^2; Observation Type: laboratory analysis; Sampling Instrument: PAR light meter.
  • Data Type: Hydrogen Sulfide (H2S) (measured); Units: millimole; Observation Type: laboratory analysis; Sampling Instrument: ion selective electrode; Sampling and Analyzing Method: Pore-water sippers were used to obtain weekly sulfide concentrations in each sample. Sulfide present in pore-water was preserved in SAOB. Pore-water samples preserved in SAOB were measured using an ion selective electrode.; Data Quality Information: Calibration curves were created to ensure collected pore-water sulfide concentrations within samples were reasonable (0-10mM).
  • Data Type: growth rate (measured); Units: mm; Observation Type: other; Sampling Instrument: ruler; Sampling and Analyzing Method: Growth rates were measured weekly for each shoot using the pin-prick method. Shoots were marked along the sheath using a 23-gauge needle. Growth is calculated as new growth per day in mm and measured over 5 days. NAs are from shoots that had died.
  • Data Type: PHOTOSYNTHETIC CAPACITY (measured); Units: unitless; Observation Type: laboratory analysis; Sampling Instrument: fluorometer; Sampling and Analyzing Method: Photosynthetic efficiency was measured weekly using a PAM fluorometer. Plants were removed from the tanks and allowed to dark adapt for 30 minutes. Measurements were taken on the inner most, or youngest, leaf just above the sheath.; Data Quality Information: PAM was not measured for week 3. Otherwise, NAs indicate a shoot that had died.
  • Data Type: OXYGEN - DISSOLVED GAS [DISSOLVED OXYGEN] (measured); Units: milligram/L; Observation Type: laboratory analysis; Sampling Instrument: Oxygen sensor; Sampling and Analyzing Method: 6 out of 12 tanks were subjected to hypoxic conditions. During the first 3 weeks of the experiment all tanks were bubbled continuously with ambient atmospheric air to maintain dissolved oxygen levels at saturation. During the latter half of the experiment, 6 of the 12 tanks were treated with 500 ppm CO2-Nitrogen gas to reduce water column oxygen to <2 mg/L (hypoxic conditions). During the last week of the experiment, oxygen concentrations in hypoxic treatments were reduced to 0 mg/L to determine if eelgrass can survive anoxia.; Data Quality Information: Oxygen concentrations in hypoxic tanks were monitored daily using PreSens PSt3 oxygen sensors and HOBO U26 Dissolved Oxygen loggers. Oxygen concentrations in oxygen-saturated tanks were monitored using Clark-type micro electrode and picoammeter.
  • Data Type: Total C:N:S (measured); Units: NA; Observation Type: laboratory analysis; Sampling Instrument: Elantech Flash EA 1112 elemental analyzer; Sampling and Analyzing Method: All samples were stored frozen (-80C) and then were dried and ground using a mortar and pestle in preparation for elemental analysis, carbon, nitrogen and sulfur, using BBOT as the standard. Sample masses ranged from 3-10 mg. Samples, blanks, and standards were run with 10 mg of vanadium pentoxide to reduce tailing of the sulfur peak.
  • Data Type: DISSOLVED INORGANIC CARBON (DIC) (measured); Units: μmol kg-1; Observation Type: laboratory analysis; Sampling Instrument: Apollo SciTech AS-C3 Dissolved Inorganic Carbon Analyzer; Sampling and Analyzing Method: Water samples were collected and filtered through a 0.2 μm nylon filter. Samples were refrigerated at 2°C for one month before processing.
Acquisition Information (collection)
Instrument
  • fluorometer
  • oxygen meter
  • PAR Sensor
Last Modified: 2018-05-08T13:03:44
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