Raw light data, coral host genotypic information and zooxanthellae type and relative abundance from coral reefs in the Florida Keys 2015-09-21 to 2016-05-26 (NCEI Accession 0175572)

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Several parameters were used to assess coral bleaching in the Florida Keys as part of NOAA’s Coral Reef Conservation Program (CRCP). The data described in this package are light, seawater pH, and coral symbiont assemblage data collected from the upper and lower Florida Keys. Light data was collected using a PAR sensor which measures light as photosynthetically active radiation (PAR, umol m-2 s-1). Seawater pH was collected using a SAMI pH sensor or a SeaBird SeaFET pH sensor. These sensors were located at the same sites as the PAR sensors and recorded pH hourly. During peak bleaching (22-25 September 2015), 20 colonies of O. faveolata were sampled with a hammer and chisel from 10 sites (n=200 total coral samples). All corals were sampled from the top of the colony given that symbiont types can change with colony orientation. A small subsample was immediately preserved in 95% ethanol for genetic analysis. Genomic DNA was extracted using the organic extraction protocol described in Rowan and Power (1991). Quantitative PCR (qPCR) assays were used to understand patterns of algal symbiont community structure to detect the presence of background symbiont types. Zooxanthellae type and abundance data are described in this package to understand the relationship between O. faveolata and Symbiodinium to assess coral bleaching responses. Symbiont assemblages in the data set are described by clades A, B, C and D since O. faveolata has been shown to associate with members from all these clades.
  • Cite as: Manzello, Derek; Enochs, Ian; Kolodziej, Graham; Valentino, Lauren; Dutra, Elizabeth; Serrano, Xaymara; Atlantic Oceanographic and Meteorological Laboratory (AOML). (2018). Raw light data, coral host genotypic information and zooxanthellae type and relative abundance from coral reefs in the Florida Keys 2015-09-21 to 2016-05-26 (NCEI Accession 0175572). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://accession.nodc.noaa.gov/0175572. Accessed [date].
gov.noaa.nodc:0175572
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Distributor DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
301-713-3277
NCEI.Info@noaa.gov
Dataset Point of Contact Information Services
DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
301-713-3277
NCEI.Info@noaa.gov
Time Period 2015-09-21 to 2016-05-26
Spatial Bounding Box Coordinates
N: 24.95375
S: 24.52202
E: -80.54806
W: -81.58263
Spatial Coverage Map
General Documentation
Associated Resources
Publication Dates
  • publication: 2018-09-11
Data Presentation Form Digital table - digital representation of facts or figures systematically displayed, especially in columns
Dataset Progress Status Complete - production of the data has been completed
Historical archive - data has been stored in an offline storage facility
Data Update Frequency As needed
Supplemental Information
Submission Package ID: M2EAWC
Purpose This dataset is available to the public for a wide variety of uses including scientific research and analysis.
Use Limitations
  • accessLevel: Public
  • Distribution liability: NOAA and NCEI make no warranty, expressed or implied, regarding these data, nor does the fact of distribution constitute such a warranty. NOAA and NCEI cannot assume liability for any damages caused by any errors or omissions in these data. If appropriate, NCEI can only certify that the data it distributes are an authentic copy of the records that were accepted for inclusion in the NCEI archives.
Dataset Citation
  • Cite as: Manzello, Derek; Enochs, Ian; Kolodziej, Graham; Valentino, Lauren; Dutra, Elizabeth; Serrano, Xaymara; Atlantic Oceanographic and Meteorological Laboratory (AOML). (2018). Raw light data, coral host genotypic information and zooxanthellae type and relative abundance from coral reefs in the Florida Keys 2015-09-21 to 2016-05-26 (NCEI Accession 0175572). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://accession.nodc.noaa.gov/0175572. Accessed [date].
Cited Authors
  • Manzello, Derek
  • Enochs, Ian
  • Kolodziej, Graham
  • Valentino, Lauren
  • Dutra, Elizabeth
  • Serrano, Xaymara
  • Atlantic Oceanographic and Meteorological Laboratory (AOML).
Principal Investigators
Collaborators
Contributors
Resource Providers
Points of Contact
Publishers
Acknowledgments
  • Related Funding Agency: US DOC; NOAA; NOS; Coral Reef Conservation Program
  • Related Funding Agency: US DOC; National Oceanic and Atmospheric Administration
Theme keywords NODC DATA TYPES THESAURUS NODC OBSERVATION TYPES THESAURUS WMO_CategoryCode
  • oceanography
CoRIS Discovery Thesaurus
  • Genetic Data Sets > PCR/QPCR Detection and Measurements
  • Numeric Data Sets > Biology
CoRIS Theme Thesaurus
  • EARTH SCIENCE > Biosphere > Vegetation > Algae > Reef Monitoring and Assessment
  • EARTH SCIENCE > Biosphere > Vegetation > Algae > Zooxanthellae
  • EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > Coral Colony Size and Condition
  • EARTH SCIENCE > Biosphere > Zoology > Corals > Reef Monitoring and Assessment > Molecular Genetic Analysis > Coral Symbiont Clade Typing
  • EARTH SCIENCE > Oceans > Ocean Chemistry > pH
  • EARTH SCIENCE > Oceans > Ocean Optics > Photosynthetically Active Radiation
Global Change Master Directory (GCMD) Science Keywords
  • EARTH SCIENCE > OCEANS > OCEAN CHEMISTRY > PH
  • EARTH SCIENCE > OCEANS > OCEAN OPTICS > PHOTOSYNTHETICALLY ACTIVE RADIATION
Data Center keywords Global Change Master Directory (GCMD) Data Center Keywords
  • DOC/NOAA/NESDIS/NODC > National Oceanographic Data Center, NESDIS, NOAA, U.S. Department of Commerce
  • DOC/NOAA/NESDIS/NCEI > National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce
  • DOC/NOAA/OAR/AOML > Atlantic Oceanographic and Meteorological Laboratory, OAR, NOAA, U.S. Department of Commerce
NODC COLLECTING INSTITUTION NAMES THESAURUS NODC SUBMITTING INSTITUTION NAMES THESAURUS Contributing Data Centers
  • AOML
  • Atlantic Oceanographic and Meterological Laboratory
Instrument keywords NODC INSTRUMENT TYPES THESAURUS Global Change Master Directory (GCMD) Instrument Keywords
  • PAR SENSORS > Photosynthetically Active Radiation Sensors
  • PH METERS
Place keywords NODC SEA AREA NAMES THESAURUS CoRIS Place Thesaurus
  • COUNTRY/TERRITORY > United States of America > Florida > Lower Florida Keys (24N081W0021)
  • COUNTRY/TERRITORY > United States of America > Florida > Upper Florida Keys (24N080W0018)
  • OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract > Lower Florida Keys (24N081W0021)
  • OCEAN BASIN > Atlantic Ocean > North Atlantic Ocean > Florida Reef Tract > Upper Florida Keys > Upper Florida Keys (24N080W0018)
Global Change Master Directory (GCMD) Location Keywords
  • OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN
  • OCEAN > ATLANTIC OCEAN > NORTH ATLANTIC OCEAN > GULF OF MEXICO
Project keywords NODC PROJECT NAMES THESAURUS CRCP Project
  • 31134
  • Elucidating the recipe for coral reef resilience in the Florida Keys
Keywords NCEI ACCESSION NUMBER
Use Constraints
  • Cite as: Manzello, Derek; Enochs, Ian; Kolodziej, Graham; Valentino, Lauren; Dutra, Elizabeth; Serrano, Xaymara; Atlantic Oceanographic and Meteorological Laboratory (AOML). (2018). Raw light data, coral host genotypic information and zooxanthellae type and relative abundance from coral reefs in the Florida Keys 2015-09-21 to 2016-05-26 (NCEI Accession 0175572). [indicate subset used]. NOAA National Centers for Environmental Information. Dataset. https://accession.nodc.noaa.gov/0175572. Accessed [date].
Access Constraints
  • Use liability: NOAA and NCEI cannot provide any warranty as to the accuracy, reliability, or completeness of furnished data. Users assume responsibility to determine the usability of these data. The user is responsible for the results of any application of this data for other than its intended purpose.
Fees
  • In most cases, electronic downloads of the data are free. However, fees may apply for custom orders, data certifications, copies of analog materials, and data distribution on physical media.
Lineage information for: dataset
Processing Steps
  • 2018-09-11T19:24:15Z - NCEI Accession 0175572 v1.1 was published.
Output Datasets
Lineage information for: dataset
Processing Steps
  • Parameter or Variable: PHOTOSYNTHETIC ACTIVE RADIATION (PAR) (measured); Units: umol m-2 s-1; Observation Category: in situ; Sampling Instrument: sensor; Sampling and Analyzing Method: Photosynthetically active radiation (PAR, 400-700nm) was measured hourly using SeaBIRD EcoPAR.
  • Parameter or Variable: pH (measured); Units: pH; Observation Category: in situ; Sampling Instrument: sensor; Sampling and Analyzing Method: Seawater pH was measured hourly. Two instruments were used. (1) SAMI-pH (2) SeaFET-pH sensor.
  • Parameter or Variable: DNA (measured); Units: count; Observation Category: laboratory analysis; Sampling Instrument: PCR machine; Sampling and Analyzing Method: Quantitative PCR (qPCR) assays were used to understand patterns of algal symbiont community structure to detect the presence of background symbiont types. Zooxanthallae type and abundance data are described in this package to understand the relationship between O. faveolata and Symbiodinium to assess coral bleaching responses. Symbiont assemblages in the data set are described by clades A, B, C and D since O. faveolata has been shown to associate with members from all these clades..
Acquisition Information (collection)
Instrument
  • PAR Sensor
  • PCR machine
  • pH sensors
Last Modified: 2019-10-25T12:45:51Z
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